Comparative chromosome mapping of the rRNA genes and telomeric repeats in three Italian pine voles of the Microtus savii s.l. complex (Rodentia, Cricetidae)

Two lines of Medicago truncatula, R-108-1 and Jemalong J5, exhibit differences in their symbiotic properties, morphological markers, and capacity for regeneration and transformation. Using cytogenetic techniques, we detected differences in chromomycin A3 staining on the two sides of the secondary constriction of the nucleolar chromosome pair of J5 and R-108-1, while DAPI (4′, 6-diamidino-2-phenylindole) staining revealed no differences between the two lines. FISH (fluorescent in situ hybridization) analysis showed a difference between J5 and R-108-1 for the same 5S rRNA genes; three loci were found in J5 but only two loci in R-108-1. However, for the 18S-5.8S-26S rDNA genes only a single locus was found in both lines. Based on these analyses, a karyotype for the two lines was established.

Download Full-text

Differentiation Of Clarias Batrachus, C. Gariepinus And Heteropneustes Fossilis By Pcr-Sequencing Of Mitochondrial 16s Rrna Gene

Journal of the Asiatic Society of Bangladesh Science ◽

10.3329/jasbs.v41i1.46190 ◽

2015 ◽

Vol 41 (1) ◽

pp. 51-58

Author(s):

Mohammad Shamimul Alam ◽

Hawa Jahan ◽

Rowshan Ara Begum ◽

Reza M Shahjahan

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Fish Larva ◽

16S Rrna Genes ◽

Rrna Genes ◽

Rrna Gene ◽

Valuable Insight ◽

Multiple Sequence ◽

Pcr Rflp ◽

Alternative Means

Heteropneustesfossilis, Clariasbatrachus and C. gariepinus are three major catfishes ofecological and economic importance. Identification of these fish species becomes aproblem when the usual external morphological features of the fish are lost or removed,such as in canned fish. Also, newly hatched fish larva is often difficult to identify. PCRsequencingprovides accurate alternative means of identification of individuals at specieslevel. So, 16S rRNA genes of three locally collected catfishes were sequenced after PCRamplification and compared with the same gene sequences available from othergeographical regions. Multiple sequence alignment of the 16S rRNA gene fragments ofthe catfish species has revealed polymorphic sites which can be used to differentiate thesethree species from one another and will provide valuable insight in choosing appropriaterestriction enzymes for PCR-RFLP based identification in future. Asiat. Soc. Bangladesh, Sci. 41(1): 51-58, June 2015

Download Full-text

Faculty Opinions recommendation of Expression of rRNA genes and nucleolus formation at ectopic chromosomal sites in the yeast Saccharomyces cerevisiae.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1033834.388004 ◽

2006 ◽

Author(s):

Jonathan R Warner

Keyword(s):

Saccharomyces Cerevisiae ◽

Rrna Genes ◽

Yeast Saccharomyces Cerevisiae

Download Full-text

Faculty Opinions recommendation of Actively transcribed rRNA genes in S. cerevisiae are organized in a specialized chromatin associated with the high-mobility group protein Hmo1 and are largely devoid of histone molecules.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1144960.602064 ◽

2009 ◽

Author(s):

Jim Maher

Keyword(s):

High Mobility ◽

High Mobility Group ◽

Rrna Genes ◽

High Mobility Group Protein ◽

Group Protein

Download Full-text

Genome sequence of Novosphingobium malaysiense strain MUSC 273T, novel alpha-proteobacterium isolated from intertidal soil

Progress In Microbes & Molecular Biology ◽

10.36877/pmmb.a0000003 ◽

2018 ◽

Vol 1 (1) ◽

Author(s):

Hooi-Leng Ser ◽

Wai-Fong Yin ◽

Kok-Gan Chan ◽

Nurul-Syakima Ab Mutalib ◽

Learn-Han Lee

Keyword(s):

Secondary Metabolites ◽

Secondary Metabolite ◽

Genome Sequence ◽

Catalase Activity ◽

Gene Clusters ◽

Rrna Genes ◽

Gram Negative ◽

Genomic Features

Novosphingobium malaysiense strain MUSC 273T is a recently identified Gram-negative, aerobic alpha-proteobacterium. The strain was isolated from intertidal soil with strong catalase activity. The genome sequence comprises 5,027,021 bp, with 50 tRNA and 3 rRNA genes. Further analysis identified presence of secondary metabolite gene clusters within genome of MUSC 273T. Knowledge of the genomic features of the strain may allow further biotechnological exploitation, particularly for production of secondary metabolites as well as production of industrially important enzymes

Download Full-text