scholarly journals Rediscovery and redescription of Niphargus enslini Karaman, 1932 (Amphipoda, Niphargidae) in southern Germany

2021 ◽  
Vol 40 ◽  
pp. 65-89
Author(s):  
Dieter Weber ◽  
Traian Brad ◽  
Fabio Stoch ◽  
Jean-François Flot
Keyword(s):  
Molecular Markers ◽  
Species Complex ◽  
Morphological Analysis ◽  
Rrna Gene ◽  
28S Rrna ◽  
Karstic Aquifer ◽  
Southern Germany ◽  
28S Rrna Gene ◽  
Putative Origin ◽  
Taxonomic Approach

Niphargus enslini Karaman, 1932 was collected only once in 1905 from the Falkensteiner Höhle (Baden-Württemberg, Germany). Two years after its description, the species was synonymized with Niphargus virei and not studied any more. During recent surveys on German niphargids, further samples collected in this cave did not yield N. enslini specimens, but this species was collected in the Blätterteighöhle and in the Schwarzer Brunnen, two caves located in Baden-Württemberg and intercepting the same karstic aquifer feeding Falkensteiner Höhle. In an integrative taxonomic approach, we carefully studied the morphology of the newly collected specimens and sequenced two molecular markers (fragments of the cytochrome c oxidase subunit I (COI) and of the nuclear 28S rRNA gene) to test for possible conspecificity of N. enslini with N. virei. Morphological analysis confirmed that N. enslini is distinct from the N. virei species complex. We provide a redescription of newly collected material, together with new drawings of a more than 100 years old topotypic female. We briefly discuss the putative origin of N. enslini and the age of its split from the N. virei species complex.

scholarly journals Intra-species sequence variability in 28s rRNA gene of Oesophagostomum venulosum isolated from goats of West Bengal, India

2010 ◽  
Vol 3 (7) ◽  
pp. 515-518 ◽  
Author(s):  
Subhasish Bandyopadhyay ◽  
Asit Kumar Bera ◽  
Sourav Sikdar ◽  
Sumanta De ◽  
Subhashree Ghosh ◽  
...  
Keyword(s):  
West Bengal ◽  
Rrna Gene ◽  
28S Rrna ◽  
Sequence Variability ◽  
28S Rrna Gene

scholarly journals Phylogenetic Analysis of 28S rRNA Gene of Indigenous Xiphinema pachydermum

2019 ◽  
Vol 8 (07) ◽  
pp. 1960-1968
Author(s):  
Anju Sharma ◽  
Satish K. Sharma ◽  
Kiran Rana ◽  
Anil Kumar Verma
Keyword(s):  
Phylogenetic Analysis ◽  
Rrna Gene ◽  
28S Rrna ◽  
28S Rrna Gene

scholarly journals Molecular and morphological characterisation of Xiphinema granatum n. sp. and Longidorus pisi Edward, Misra & Singh, 1964 (Dorylaimida: Longidoridae) from Iran

Nematology ◽  
2012 ◽  
Vol 14 (8) ◽  
pp. 949-960 ◽  
Author(s):  
Majid Pedram ◽  
Ebrahim Pourjam ◽  
Juan E. Palomares-Rius ◽  
Razieh Ghaemi ◽  
Carolina Cantalapiedra-Navarrete ◽  
...  
Keyword(s):  
New Species ◽  
Species Group ◽  
Rrna Gene ◽  
28S Rrna ◽  
Morphometric Data ◽  
Molecular Analyses ◽  
Morphological Characterisation ◽  
28S Rrna Gene ◽  
Close Relationship ◽  
Female Genital

Xiphinema granatum n. sp. from pomegranate in Saveh, Markazi province, central Iran, is described and illustrated. The new species belongs to species group 8 of the genus Xiphinema sensu Loof & Luc, 1990. The new species is characterised by a body 3.5-4.1 mm long, 37-49 μm diam., odontostyle and odontophore 118-132 and 65-74 μm long, respectively, presence of sperm in the female genital tracts, uterus devoid of any Z-differentiation or spines, and abundant males with spicules 45-58 μm long. The polytomous identification codes of the new species are: A4, B4, C5a, D5, E56, F4, G23, H2, I3, J4, K2, L2. Beside morphological and morphometric data, molecular analyses of D2-D3 fragments of 28S rRNA gene placed the new species in close relationship with X. vuittenezi and X. italiae and some species belonging to Xiphinema morphospecies group 6, but clearly separated from X. index. In addition, an Iranian population of Longidorus pisi from the same locality is morphologically and molecularly characterised.

Placement of Cacoceria and phylogenetic relationships of the xylotine genera of the tribe Milesiini (Diptera, Syrphidae: Eristalinae) based on molecular characters

Zootaxa ◽  
2006 ◽  
Vol 1171 (1) ◽  
pp. 17 ◽  
Author(s):  
GUNILLA STÅHLS
Keyword(s):  
Intraspecific Variation ◽  
Phylogenetic Relationships ◽  
Rrna Gene ◽  
28S Rrna ◽  
Large Fragment ◽  
Mitochondrial Coi ◽  
Phylogenetic Placement ◽  
28S Rrna Gene ◽  
Molecular Characters

The phylogenetic relationships of traditional xylotine genera of tribe Milesiini (Diptera, Syrphidae: Eristalinae) were explored using molecular character of a large fragment of the mitochondrial COI and the D2-3 region of the nuclear ribosomal 28S rRNA gene. Of particular interest was the phylogenetic placement of the enigmatic Neotropical genus Cacoceria. The ingroup included 48 taxa, and Cheilosia illustrata (Rhingiini, Eristalinae) was used as outgroup. Multiple specimens of more common taxa were sequenced for surveying levels of intraspecific variation. The combined dataset was analysed using parsimony and optimisation alignment, using the program POY. Cacoceria was resolved within the Chalcosyrphus clade. Earlier hypotheses placed the taxon in the tribe Myoleptini or Chrysogasterini, or in Xylotini without stating a closer relationship to any particular xylotine taxon. The representatives of traditional xylotine taxa were resolved as ((Hadromyia + (Brachypalpoides + Blera + Lejota) + (Xylota sg. Hovaxylota + Xylota sg. Sterphoides) + (Xylota (including Sterphus)) while Neplas and Brachypalpus were resolved in the Myoleptini albeit with very low support.

scholarly journals Reconstruction of the phylogeny of the Opisthobranchia (Mollusca: Gastropoda) by means of 18s and 28s rRNA gene sequences

2005 ◽  
Vol 71 (2) ◽  
pp. 113-125 ◽  
Author(s):  
Verena Vonnemann ◽  
Michael Schrödl ◽  
Annette Klussmann-Kolb ◽  
Heike Wägele
Keyword(s):  
Rrna Gene ◽  
28S Rrna ◽  
Gene Sequences ◽  
28S Rrna Gene

Phylogenetic position of the genus Mesochytrium (Chytridiomycota) based on zoospore ultrastructure and sequences from the 18S and 28S rRNA gene

Nova Hedwigia ◽  
2010 ◽  
Vol 90 (1) ◽  
pp. 81-94 ◽  
Author(s):  
Sergey A. Karpov ◽  
Peter M. Letcher ◽  
Maria A. Mamkaeva ◽  
Kira A. Mamkaeva
Keyword(s):  
Phylogenetic Position ◽  
Rrna Gene ◽  
28S Rrna ◽  
28S Rrna Gene

Phylogenetic origin of the Monotropoideae inferred from partial 28S ribosomal RNA gene sequences

1992 ◽  
Vol 70 (8) ◽  
pp. 1703-1708 ◽  
Author(s):  
K. W. Cullings ◽  
T. D. Bruns
Keyword(s):  
Ribosomal Rna ◽  
Recent Common Ancestor ◽  
Gradual Transition ◽  
Rrna Gene ◽  
28S Rrna ◽  
28S Rrna Gene ◽  
Most Recent Common Ancestor ◽  
Alternative Hypotheses ◽  
Degree Of Confidence ◽  
Phylogenetic Origin

Despite more than 100 years of detailed analysis of morphology and macromolecules, the phylogenetic origin of the Monotropoideae remains unclear. In this study partial sequences from the 28S rRNA gene were used to test two alternative hypotheses: (i) that the Monotropoideae share a most recent common ancestor with the Arbutoideae in the Ericaceae or (ii) that the Monotropoideae is the end result of a gradual transition from autotrophism to mycotrophic parasitism in the Pyrolaceae. Our results support the hypothesis that the Monotropoideae and the Arbutoideae are a monophyletic group and that the Monotropoideae should be included in the Ericaceae rather than the Pyrolaceae. The Monotropoid species Pleuricospora fimbriolata could not be placed within either the Monotropoideae or the Arbutoideae with any degree of confidence, leaving open the possibility that the Monotropoideae is polyphyletic. Key words: Monotropoideae, Ericaceae, 28S rRNA gene, PCR, molecular systematics.

scholarly journals Identification of Scopulariopsis Species by Partial 28S rRNA Gene Sequence Analysis

2013 ◽  
Vol 62 (3) ◽  
pp. 303-306 ◽  
Author(s):  
TOMASZ JAGIELSKI ◽  
KINGA KOSIM ◽  
MAGDALENA SKÓRA ◽  
ANNA BARBARA MACURA ◽  
JACEK BIELECKI
Keyword(s):  
Sequence Analysis ◽  
Gene Sequence ◽  
Systemic Disease ◽  
Molecular Diagnostic ◽  
Rrna Gene ◽  
28S Rrna ◽  
Opportunistic Pathogens ◽  
Deep Tissue ◽  
28S Rrna Gene ◽  
Gene Sequence Analysis

The genus Scopulariopsis contains over 30 species of mitosporic moulds, which although usually saprophytic may also act as opportunistic pathogens in humans. They have mainly been associated with onychomycosis, and only sporadically reported as a cause of deep tissue infections or systemic disease. Identification of Scopulariopsis species still largely relies on phenotype-based methods. There is a need for a molecular diagnostic approach, that would allow to reliably discriminate between different Scopulariopsis species. The aim of this study was to apply sequence analysis of partial 28S rRNA gene for species identification of Scopulariopsis clinical isolates. Although the method employed did reveal some genetic polymorphism among Scopulariopsis isolates tested, it was not enough for species delineation. For this to be achieved, other genetic loci, within and beyond the rDNA operon, need to be investigated.

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