Regulation of Chondrogenesis by Transforming Growth Factor-ß3 and Insulin-like Growth Factor-1 from Human Mesenchymal Umbilical Cord Blood Cells

2010 ◽  
Vol 37 (7) ◽  
pp. 1519-1526 ◽  
Author(s):  
CRISTIANE S. MARA ◽  
ADRIANA S.S. DUARTE ◽  
ANGELICA SARTORI ◽  
ANGELA C. LUZO ◽  
SARA T.O. SAAD ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Transforming Growth Factor ◽  
Type Ii Collagen ◽  
Micromass Culture ◽  
Insulin Like Growth Factor ◽  
Type Ii ◽  
Cord Blood Cells

Objective.Mature articular cartilage is vulnerable to injuries and disease processes that cause irreversible tissue damage because of its limited capacity for self-repair. Umbilical cord blood is a source of mesenchymal stem cells, which can give rise to cells of different lineages, including cartilage, bone, and fat. Cellular condensation is a required step in the initiation of mesenchymal chondrogenesis. We attempted to differentiate cells from umbilical cord blood into chondrocytes with insulin-like growth factor 1 (IGF-1) and transforming growth factor-ß3 (TGF-ß3).Methods.Cells were grown in high density micromass and monolayer culture systems and then evaluated for expression of type II collagen, aggrecan, and Sox9. Umbilical cord blood from 130 patients was harvested.Results.Expression of type II collagen, aggrecan, and Sox9 was detected after 14 days in TGF-ß3- and IGF-1-stimulated cells in both types of culture (monolayer and micromass). On Day 21 in the micromass culture, expression levels were greater than they were at 14 days for all genes. TGF-ß3 was found to be more efficient at promoting chondrogenesis than IGF-1. By western blot, we also found that after 3 weeks, the expression of type II collagen was greater in micromass culture with TGF-ß3.Conclusion.TGF-ß3 used in micromass culture is the best growth factor for promoting the proliferation and differentiation of mesenchymal cells from umbilical cord blood during chondrogenesis. This approach may provide an alternative to autologous grafting.

GLYCOSYLATED FIBRONECTIN AND INHIBIN ARE LOWER AND ANTI-MÜLLERIAN HORMONE IS HIGHER IN UMBILICAL CORD BLOOD WHEN MOTHERS HAVE PREECLAMPSIA

2020 ◽  
Vol 26 (3) ◽  
pp. 318-327
Author(s):  
Bridget DiPrisco ◽  
Ajay Kumar ◽  
Bhanu Kalra ◽  
Gopal V. Savjani ◽  
Zoe Michael ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Immunosorbent Assay ◽  
Plasma Protein ◽  
Umbilical Cord Blood ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blood Levels ◽  
Insulin Like Growth Factor ◽  
Tertiary Teaching

Objective: Preeclampsia is a common disorder of pregnancy, causing significant morbidity and mortality for mothers and infants. Several molecules, including glycosylated fibronectin (GlyFn), the inhibin-related proteins, anti-müllerian hormone (AMH), and the insulin-like growth factor axis, are altered in maternal plasma in the setting of preeclampsia; however, these molecules have not been previously measured in cord blood of infants born to mothers with preeclampsia, which may represent changes in fetal physiology. We evaluated potential biomarkers of preeclampsia in umbilical cord blood to fill the gap in knowledge. Methods: This is a case-control study of 196 neonates born at a tertiary teaching hospital in Boston from 2010–2017. Forty-nine neonates born to mothers with preeclampsia were matched 1:3 by gestational age, sex, and birth weight z-score with 147 controls. Eleven analytes were measured in cord blood by enzyme-linked immunosorbent assay. Binary logistic regression analyses were performed to evaluate associations between preeclampsia and analytes. Results: Mean cord blood levels of GlyFn and total inhibin were significantly lower in neonates born to mothers with preeclampsia compared to controls, and AMH levels were significantly higher in males born to mothers with preeclampsia than male controls. Associations remained significant after controlling for maternal and neonatal characteristics. Conclusion: Cord blood levels of GlyFn and inhibin are decreased and AMH (male) levels are increased in infants of preeclamptic mothers, which is opposite the pattern these biomarkers show in serum of mothers with preeclampsia. These molecules may be important in the pathophysiology and long-term effects of preeclampsia on the developing fetus. Abbreviations: AMH = anti-müllerian hormone; ELISA = enzyme-linked immunosorbent assay; GlyFn = glycosylated fibronectin; IGF = insulin-like growth factor; IGFBP5 = insulin-like growth factor binding protein 5; LOD = limit of detection; PAPP-A = pregnancy-associated plasma protein A; PAPP-A2 = pregnancy-associated plasma protein A2

scholarly journals Human Umbilical Cord Blood Cells can be Induced to Express Markers for Neurons and Glia

2002 ◽  
Vol 11 (3) ◽  
pp. 261-264 ◽  
Author(s):  
Alma R. Bicknese ◽  
Holly S. Goodwin ◽  
Cheryl O. Quinn ◽  
Verneake C. D. Henderson ◽  
Shin-Nan Chien ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Multipotent Stem Cells ◽  
Cord Blood Cells ◽  
Umbilical Cord Blood Cells

Rare cells are present in human umbilical cord blood that do not express the hematopoietic marker CD45 and in culture do not produce cells of hematopoietic lineage. These umbilical cord multipotent stem cells (UC-MC) behave as multilineage progenitor cells (stem cells) and can be expanded in tissue culture. Exposure to basic fibroblast growth factor (bFGF) and human epidermal growth factor (hEGF) for a minimum of 7 days in culture induces expression of neural and glial markers. Western immunoblots demonstrate expression of both β-tubulin III and glial fibrillary acidic protein (GFAP). Immunocytochemistry of the cells showed intense labeling to both compounds on the intracellular cytoskeleton. The oligodendrocyte cell surface marker galactocerebroside (Gal-C) was present on most cells. Many cells show dual labeling, expressing both neuronal and glial markers.

STUDIES OF INSULIN-LIKE GROWTH FACTOR -I AND -II BY SPECIFIC RADIOLIGAND ASSAYS IN UMBILICAL CORD BLOOD

1983 ◽  
Vol 19 (3) ◽  
pp. 405-413 ◽  
Author(s):  
P. D. GLUCKMAN ◽  
J. J. JOHNSON-BARRETT ◽  
J. H. BUTLER ◽  
B. W. EDGAR ◽  
T. R. GUNN
Keyword(s):  
Growth Factor ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Growth Factor I

scholarly journals Racial variation in umbilical cord blood sex steroid hormones and the insulin-like growth factor axis in African-American and white female neonates

2012 ◽  
Vol 23 (3) ◽  
pp. 445-454 ◽  
Author(s):  
Tanya Agurs-Collins ◽  
Sabine Rohrmann ◽  
Catherine Sutcliffe ◽  
Jessica L. Bienstock ◽  
Deborah Monsegue ◽  
...  
Keyword(s):  
African American ◽  
Growth Factor ◽  
Cord Blood ◽  
Steroid Hormones ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Sex Steroid Hormones ◽  
Sex Steroid ◽  
White Female ◽  
Insulin Like Growth Factor

scholarly journals Racial Variation in Sex Steroid Hormones and the Insulin-Like Growth Factor Axis in Umbilical Cord Blood of Male Neonates

2009 ◽  
Vol 18 (5) ◽  
pp. 1484-1491 ◽  
Author(s):  
Sabine Rohrmann ◽  
Catherine G. Sutcliffe ◽  
Jessica L. Bienstock ◽  
Deborah Monsegue ◽  
Folasade Akereyeni ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cord Blood ◽  
Steroid Hormones ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Sex Steroid Hormones ◽  
Sex Steroid ◽  
Insulin Like Growth Factor ◽  
Racial Variation

Reproducibility of assays for steroid hormones, prolactin and insulin-like growth factor-1 in umbilical cord blood

2006 ◽  
Vol 20 (1) ◽  
pp. 79-86 ◽  
Author(s):  
Inkyung Baik ◽  
Qin Liu ◽  
Susan Sturgeon ◽  
Edward J. Stanek ◽  
William Okulicz ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cord Blood ◽  
Steroid Hormones ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Insulin Like Growth Factor
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