The sheep (Ovis aries) mammary gland mitochondrial complexes: establishment of a Blue-Native PAGE separation method as a model for other ruminant species

2013 ◽  
pp. 196-198
Author(s):  
Carlos Filipe ◽  
Vitor Vasconcelos ◽  
André M. Almeida ◽  
Alexandre Campos
PLoS ONE ◽  
2016 ◽  
Vol 11 (3) ◽  
pp. e0151599 ◽  
Author(s):  
Graziano Cugno ◽  
José R. Parreira ◽  
Enea Ferlizza ◽  
Lorenzo E. Hernández-Castellano ◽  
Mariana Carneiro ◽  
...  

2011 ◽  
Vol 166 (1) ◽  
pp. 47-62 ◽  
Author(s):  
Jiri Dresler ◽  
Jana Klimentova ◽  
Jiri Stulik

2006 ◽  
Vol 1 (1) ◽  
pp. 418-428 ◽  
Author(s):  
Ilka Wittig ◽  
Hans-Peter Braun ◽  
Hermann Schägger

2017 ◽  
Vol 112 (3) ◽  
pp. 477a
Author(s):  
Anke Dopychai ◽  
Ralf Hausmann ◽  
Linda Krüger ◽  
Stefan Gründer ◽  
Günther Schmalzing

2004 ◽  
Vol 121 (4) ◽  
pp. 546-555 ◽  
Author(s):  
Jonas Kjell ◽  
Allan G. Rasmusson ◽  
Hakan Larsson ◽  
Susanne Widell

2008 ◽  
pp. 557-569
Author(s):  
Holger Eubel ◽  
A. Harvey Millar

PROTEOMICS ◽  
2012 ◽  
Vol 12 (17) ◽  
pp. 2729-2738 ◽  
Author(s):  
Adelson Joel da Silva ◽  
Diana Paola Gómez-Mendoza ◽  
Magno Junqueira ◽  
Gilberto Barbosa Domont ◽  
Edivaldo Ximenes Ferreira Filho ◽  
...  

2012 ◽  
Vol 445 (2) ◽  
pp. 237-246 ◽  
Author(s):  
Yusuke Kakizaki ◽  
Anthony L. Moore ◽  
Kikukatsu Ito

Symplocarpus renifolius and Arum maculatum are known to produce significant heat during the course of their floral development, but they use different regulatory mechanisms, i.e. homoeothermic compared with transient thermogenesis. To further clarify the molecular basis of species-specific thermogenesis in plants, in the present study we have analysed the native structures and expression patterns of the mitochondrial respiratory components in S. renifolius and A. maculatum. Our comparative analysis using Blue native PAGE combined with nano LC (liquid chromatography)-MS/MS (tandem MS) has revealed that the constituents of the respiratory complexes in both plants were basically similar, but that several mitochondrial components appeared to be differently expressed in their thermogenic organs. Namely, complex II in S. renifolius was detected as a 340 kDa product, suggesting an oligomeric or supramolecular structure in vivo. Moreover, the expression of an external NAD(P)H dehydrogenase was found to be higher in A. maculatum than in S. renifolius, whereas an internal NAD(P)H dehydrogenase was expressed at a similar level in both species. Alternative oxidase was detected as smear-like signals that were elongated on the first dimension with a peak at around 200 kDa in both species. The significance and implication of these data are discussed in terms of thermoregulation in plants.


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