The sheep (Ovis aries) mammary gland mitochondrial complexes: establishment of a Blue-Native PAGE separation method as a model for other ruminant species

2013 ◽  
pp. 196-198
Author(s):  
Carlos Filipe ◽  
Vitor Vasconcelos ◽  
André M. Almeida ◽  
Alexandre Campos
Keyword(s):  
Mammary Gland ◽  
Ovis Aries ◽  
Separation Method ◽  
Native Page ◽  
Ruminant Species ◽  
Blue Native Page ◽  
Mitochondrial Complexes ◽  
Blue Native

scholarly journals The Goat (Capra hircus) Mammary Gland Mitochondrial Proteome: A Study on the Effect of Weight Loss Using Blue-Native PAGE and Two-Dimensional Gel Electrophoresis

PLoS ONE ◽  
2016 ◽  
Vol 11 (3) ◽  
pp. e0151599 ◽  
Author(s):  
Graziano Cugno ◽  
José R. Parreira ◽  
Enea Ferlizza ◽  
Lorenzo E. Hernández-Castellano ◽  
Mariana Carneiro ◽  
...  
Keyword(s):  
Weight Loss ◽  
Mammary Gland ◽  
Gel Electrophoresis ◽  
Capra Hircus ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Native Page ◽  
Mitochondrial Proteome ◽  
Blue Native Page ◽  
Blue Native

scholarly journals Bacterial protein complexes investigation using blue native PAGE

2011 ◽  
Vol 166 (1) ◽  
pp. 47-62 ◽  
Author(s):  
Jiri Dresler ◽  
Jana Klimentova ◽  
Jiri Stulik
Keyword(s):  
Protein Complexes ◽  
Bacterial Protein ◽  
Native Page ◽  
Blue Native Page ◽  
Blue Native

Blue native PAGE

2006 ◽  
Vol 1 (1) ◽  
pp. 418-428 ◽  
Author(s):  
Ilka Wittig ◽  
Hans-Peter Braun ◽  
Hermann Schägger
Keyword(s):  
Native Page ◽  
Blue Native Page ◽  
Blue Native

scholarly journals Blue Native Page Evidence that Plasma Membrane-Bound Human ENaC Channels Share a Trimeric Architecture with Full-Length Human ASIC1 and ASIC2

2017 ◽  
Vol 112 (3) ◽  
pp. 477a
Author(s):  
Anke Dopychai ◽  
Ralf Hausmann ◽  
Linda Krüger ◽  
Stefan Gründer ◽  
Günther Schmalzing
Keyword(s):  
Plasma Membrane ◽  
Full Length ◽  
Native Page ◽  
Blue Native Page ◽  
Membrane Bound ◽  
Blue Native

Protein complexes of the plant plasma membrane resolved by Blue Native PAGE

2004 ◽  
Vol 121 (4) ◽  
pp. 546-555 ◽  
Author(s):  
Jonas Kjell ◽  
Allan G. Rasmusson ◽  
Hakan Larsson ◽  
Susanne Widell
Keyword(s):  
Plasma Membrane ◽  
Protein Complexes ◽  
Native Page ◽  
Blue Native Page ◽  
Blue Native ◽  
Plant Plasma Membrane

Blue-Native Page in Studying Protein Complexes

2008 ◽  
pp. 557-569
Author(s):  
Holger Eubel ◽  
A. Harvey Millar
Keyword(s):  
Protein Complexes ◽  
Native Page ◽  
Blue Native Page ◽  
Blue Native

Blue native-PAGE analysis of Trichoderma harzianum secretome reveals cellulases and hemicellulases working as multienzymatic complexes

PROTEOMICS ◽  
2012 ◽  
Vol 12 (17) ◽  
pp. 2729-2738 ◽  
Author(s):  
Adelson Joel da Silva ◽  
Diana Paola Gómez-Mendoza ◽  
Magno Junqueira ◽  
Gilberto Barbosa Domont ◽  
Edivaldo Ximenes Ferreira Filho ◽  
...  
Keyword(s):  
Trichoderma Harzianum ◽  
Native Page ◽  
Blue Native Page ◽  
Blue Native

scholarly journals Different molecular bases underlie the mitochondrial respiratory activity in the homoeothermic spadices of Symplocarpus renifolius and the transiently thermogenic appendices of Arum maculatum

2012 ◽  
Vol 445 (2) ◽  
pp. 237-246 ◽  
Author(s):  
Yusuke Kakizaki ◽  
Anthony L. Moore ◽  
Kikukatsu Ito
Keyword(s):  
Expression Patterns ◽  
Native Page ◽  
Blue Native Page ◽  
Arum Maculatum ◽  
Species Specific ◽  
Molecular Bases ◽  
Blue Native ◽  
Mitochondrial Respiratory Activity ◽  
Mitochondrial Respiratory

Symplocarpus renifolius and Arum maculatum are known to produce significant heat during the course of their floral development, but they use different regulatory mechanisms, i.e. homoeothermic compared with transient thermogenesis. To further clarify the molecular basis of species-specific thermogenesis in plants, in the present study we have analysed the native structures and expression patterns of the mitochondrial respiratory components in S. renifolius and A. maculatum. Our comparative analysis using Blue native PAGE combined with nano LC (liquid chromatography)-MS/MS (tandem MS) has revealed that the constituents of the respiratory complexes in both plants were basically similar, but that several mitochondrial components appeared to be differently expressed in their thermogenic organs. Namely, complex II in S. renifolius was detected as a 340 kDa product, suggesting an oligomeric or supramolecular structure in vivo. Moreover, the expression of an external NAD(P)H dehydrogenase was found to be higher in A. maculatum than in S. renifolius, whereas an internal NAD(P)H dehydrogenase was expressed at a similar level in both species. Alternative oxidase was detected as smear-like signals that were elongated on the first dimension with a peak at around 200 kDa in both species. The significance and implication of these data are discussed in terms of thermoregulation in plants.

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