Free and hidden fumonisins in Argentinean raw maize samples

2020 ◽  
Vol 13 (1) ◽  
pp. 109-116
Author(s):  
P.S. Pok ◽  
V.A. García Londoño ◽  
S.P. Aransibia ◽  
S. Vicente ◽  
A.M. Pacín ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
European Commission ◽  
Alkaline Hydrolysis ◽  
High Performance ◽  
Fluorescence Detector ◽  
Analytical Methodology ◽  
Limits Of Detection

The concentrations of free fumonisins (FBs) and hydrolysed fumonisins (HFBs) were determined in 72 maize samples collected in 2017 from five Argentine provinces. The methodology for HFBs analysis consisted of alkaline hydrolysis followed by high performance liquid chromatography with a fluorescence detector (HPLC-FLD). The developed analytical methodology presented percentages of recovery greater than 74%. Limits of detection were 4.5, 13.0 and 12.9 μg/kg for HFB1, HFB2 and HFB3, respectively. Presence of FBs was found in 86% of the samples. In all cases, the concentration of total HFBs (after the hydrolysis treatment) was superior to the free FBs content (HFBs to FBs median ratio of 2.5), which indicates the presence of hidden fumonisins in Argentinean maize. 8% of the traditionally analysed samples exceeded the limit established by the European Commission for FB1 + FB2. When applying alkaline hydrolysis to the samples, 24% of them exceed this limit.

HPLC Analysis of Oxytetracycline Residues in Honey

1986 ◽  
Vol 49 (5) ◽  
pp. 383-388 ◽  
Author(s):  
PETER SPORNS ◽  
SUET KWAN ◽  
LAWRENCE A. ROTH
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Aqueous Solutions ◽  
High Performance ◽  
Hplc Analysis ◽  
Extraction Procedure ◽  
Hplc Method ◽  
Limits Of Detection ◽  
Ph Values ◽  
Double Extraction

Oxytetracycline (OTC), also known commercially as Terramycin, was determined to be more stable in honey than in buffered aqueous solutions at similar pH values and temperatures. A rapid high performance liquid chromatography (HPLC) method was developed to detect and quantitate OTC using a 1:1 dilution (wt/wt) of honey samples in water. Using 355 nm as the wavelength of detection, amounts as low as 0.5 μg/ml could be detected in the above solution. The limits of detection were lowered considerably by a double extraction procedure.

Salting-out induced liquid–liquid microextraction based on the system of acetonitrile/magnesium sulfate for trace-level quantitative analysis of fluoroquinolones in water, food and biological matrices by high-performance liquid chromatography with a fluorescence detector

2014 ◽  
Vol 6 (17) ◽  
pp. 6973-6980 ◽  
Author(s):  
Dongli Du ◽  
Guozhong Dong ◽  
Yuanyuan Wu ◽  
Jingjing Wang ◽  
Ming Gao ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
High Performance ◽  
Biological Matrices ◽  
Fluorescence Detector ◽  
Trace Level ◽  
Salting Out ◽  
Liquid Microextraction

A convenient, economical SILLME method coupled with HPLC/FLD for sample preparation, extraction and trace-level quantitative determination of fluoroquinolones is reported.

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