Meat Adulteration in Cooked Mutton Kebab with Cattle and Buffalo Meat and its Detection Using Mitochondrial DNA (mtDNA) Based Multiplex PCR

2016 ◽  
Vol 11 (8) ◽  
pp. 505-510
Author(s):  
M. Mansoor Bhat ◽  
Imtiyaz A. Mantoo ◽  
Mir Salahuddin ◽  
Sheikh Adil ◽  
M. Ashraf Pal
Keyword(s):  
Mitochondrial Dna ◽  
Multiplex Pcr ◽  
Buffalo Meat

scholarly journals Multiplex PCR-based genotyping of mitochondrial DNA from two species of the genus Batillaria (B. multiformis and B. flectosiphonata) from Amami-Oshima, Japan

2014 ◽  
Vol 9 (1) ◽  
pp. 67-70 ◽  
Author(s):  
Kimiko Hirose ◽  
Takefumi Yorisue ◽  
Hajime Itoh ◽  
Tomoko Yamamoto ◽  
Shigeaki Kojima
Keyword(s):  
Mitochondrial Dna ◽  
Multiplex Pcr

scholarly journals Direct detection of multiple point mutations in mitochondrial DNA

1997 ◽  
Vol 43 (10) ◽  
pp. 1857-1861 ◽  
Author(s):  
Lee-Jun C Wong ◽  
Dinithi Senadheera
Keyword(s):  
Mitochondrial Dna ◽  
Multiplex Pcr ◽  
Direct Detection ◽  
Point Mutations ◽  
Cost Effective ◽  
Large Deletion ◽  
Multiple Point ◽  
Mtdna Mutations ◽  
Allele Specific ◽  
Mitochondrial Defects

Abstract Mitochondrial defects can be caused by mutations in nuclear or mitochondrial DNA. Large deletion/duplication and point mutations are the two major types of mitochondrial DNA (mtDNA) mutations. Comprehensive molecular diagnosis requires the analysis of multiple point mutations. We developed an effective multiplex PCR/allele-specific oligonucleotide (ASO) method to simultaneously screen multiple point mutations in mtDNA. The system involved three pairs of primers to amplify mutation “hot spots” at tRNAleu(UUR), tRNAlys/ATPase, and ND4 regions, followed by detection of point mutations with ASO probes. Over 2000 specimens were analyzed and the results were compared with those from previous studies with the PCR/restriction fragment length polymorphism method. Our data demonstrate that the multiplex PCR/ASO method is much more sensitive in the detection of low mutant heteroplasmy. It is simple and cost effective, especially if a large number of samples are to be screened for multiple point mutations.

A new multiplex-PCR comprising autosomal and y-specific STRs and mitochondrial DNA to analyze highly degraded material

2009 ◽  
Vol 3 (2) ◽  
pp. 96-103 ◽  
Author(s):  
Nicole von Wurmb-Schwark ◽  
Andrea Preusse-Prange ◽  
Anke Heinrich ◽  
Eva Simeoni ◽  
Thomas Bosch ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Multiplex Pcr ◽  
Degraded Material

scholarly journals Harnessing multiplex PCR assay targeting specific mitochondrial DNA elements for simultaneous identification of antelope species in Cornu Saigae Tataricae

2019 ◽  
Vol 4 (2) ◽  
pp. 3222-3226
Author(s):  
Yufei Chen ◽  
Yaya Yang ◽  
Yinhuan Qian ◽  
Roselyn Tehzee Gblinwon ◽  
Zhaoqun Jiao ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Multiplex Pcr ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Dna Elements ◽  
Simultaneous Identification

scholarly journals Study of multiplex PCR and high-speed PCR of mitochondrial DNA analysis

2018 ◽  
Vol 23 (2) ◽  
pp. 103-112 ◽  
Author(s):  
Yusuke Mita ◽  
Tetsushi Kitayama ◽  
Takashi Fukagawa ◽  
Haruhiko Watahiki ◽  
Koji Fujii ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Multiplex Pcr ◽  
High Speed ◽  
Dna Analysis ◽  
Mitochondrial Dna Analysis

A single multiplex PCR and SNaPshot minisequencing reaction of 42 SNPs to classify admixture populations into mitochondrial DNA haplogroups

Mitochondrion ◽  
2011 ◽  
Vol 11 (2) ◽  
pp. 296-302 ◽  
Author(s):  
Greiciane G. Paneto ◽  
Stephan Köhnemann ◽  
Joyce A. Martins ◽  
Regina M.B. Cicarelli ◽  
Heidi Pfeiffer
Keyword(s):  
Mitochondrial Dna ◽  
Multiplex Pcr ◽  
Snapshot Minisequencing

Effect of Meat Processing and Cooking on DNA Extraction and Detection of Meat Adulteration in Mutton Rista (Kashmiri Meat Product)

2016 ◽  
Vol 53 (4) ◽  
pp. 459
Author(s):  
Mohammad Mansoor Bhat ◽  
Heena Jalal ◽  
Parveez Ahmad Para ◽  
Subha Ganguly
Keyword(s):  
Multiplex Pcr ◽  
Dna Extraction ◽  
Dna Isolation ◽  
Meat Product ◽  
Pcr Analysis ◽  
Meat Processing ◽  
Buffalo Meat ◽  
Study Material ◽  
Dna Quality ◽  
Meat Samples

The processing and cooking of meat, during meat product preparation, affects the DNA quality and its concentration during DNA isolation. In this study, the effect of processing and cooking, during Rista preparation, on meat speciation of beef and buffalo meat in mutton Rista was studied. The study material involved three types of meat i.e. unprocessed meat, Rista emulsion and the final cooked Rista product. In each type of meat, pure meat samples of mutton, beef and buffalo meat were studied along with the adulterated mutton sample having 10% beef and 10% buffalo meat adulteration level. The meat samples were subjected to mtDNA isolation and multiplex PCR analysis. The results of this study showed that processing and cooking decreases the concentration of extracted DNAs but does not affect the detection of beef and buffalo meat in adulterated mutton Rista (unprocessed, processed and cooked) at 10% level of adulteration.

Sign in / Sign up

Export Citation Format

Share Document