Effect of Minerals Salts in Fermentation Process using Mango Residues as Carbon Source for Bioethanol Production

2010 ◽  
Vol 3 (1) ◽  
pp. 29-38 ◽  
Author(s):  
Marius Kounbesiou ◽  
Aly Savadogo ◽  
Nicolas Barro ◽  
Philippe Thonart ◽  
Alfred Sabadenedy
Keyword(s):  
Carbon Source ◽  
Fermentation Process ◽  
Bioethanol Production

scholarly journals A microbubble-sparged yeast propagation–fermentation process for bioethanol production

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Vijayendran Raghavendran ◽  
Joseph P. Webb ◽  
Michaël L. Cartron ◽  
Vicki Springthorpe ◽  
Tony R. Larson ◽  
...  
Keyword(s):  
Fermentation Process ◽  
Bioethanol Production

scholarly journals N-Acetylglucosamine Utilization by Saccharomyces cerevisiae Based on Expression of Candida albicans NAG Genes

2009 ◽  
Vol 75 (18) ◽  
pp. 5840-5845 ◽  
Author(s):  
Jürgen Wendland ◽  
Yvonne Schaub ◽  
Andrea Walther
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Candida Albicans ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
De Novo ◽  
Biofuel Production ◽  
Bioethanol Production ◽  
Catabolic Pathway ◽  
Cellular Functions ◽  
Kinase A

ABSTRACT Synthesis of chitin de novo from glucose involves a linear pathway in Saccharomyces cerevisiae. Several of the pathway genes, including GNA1, are essential. Genes for chitin catabolism are absent in S. cerevisiae. Therefore, S. cerevisiae cannot use chitin as a carbon source. Chitin is the second most abundant polysaccharide after cellulose and consists of N-acetylglucosamine (GlcNAc) moieties. Here, we have generated S. cerevisiae strains that are able to use GlcNAc as a carbon source by expressing four Candida albicans genes (NAG3 or its NAG4 paralog, NAG5, NAG2, and NAG1) encoding a GlcNAc permease, a GlcNAc kinase, a GlcNAc-6-phosphate deacetylase, and a glucosamine-6-phosphate deaminase, respectively. Expression of NAG3 and NAG5 or NAG4 and NAG5 in S. cerevisiae resulted in strains in which the otherwise-essential ScGNA1 could be deleted. These strains required the presence of GlcNAc in the medium, indicating that uptake of GlcNAc and its phosphorylation were achieved. Expression of all four NAG genes produced strains that could use GlcNAc as the sole carbon source for growth. Utilization of a GlcNAc catabolic pathway for bioethanol production using these strains was tested. However, fermentation was slow and yielded only minor amounts of ethanol (approximately 3.0 g/liter), suggesting that fructose-6-phosphate produced from GlcNAc under these conditions is largely consumed to maintain cellular functions and promote growth. Our results present the first step toward tapping a novel, renewable carbon source for biofuel production.

scholarly journals Enhancing the Feasibility ofMicrocystis aeruginosaas a Feedstock for Bioethanol Production under the Influence of Various Factors

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Muhammad Imran Khan ◽  
Moon Geon Lee ◽  
Hyo Jin Seo ◽  
Jin Hyuk Shin ◽  
Tai Sun Shin ◽  
...  
Keyword(s):  
Growth Rate ◽  
Fermentation Process ◽  
Aminolevulinic Acid ◽  
Pichia Stipitis ◽  
Naphthalene Acetic Acid ◽  
Bioethanol Production ◽  
Algae Biomass ◽  
Pretreatment Method ◽  
Red Led ◽  
Different Types

Microcystis aeruginosa, a freshwater microalga, is capable of producing and accumulating different types of sugars in its biomass which make it a good feedstock for bioethanol production. Present study aims to investigate the effect of different factors increasing growth rate and carbohydrates productivity ofM. aeruginosa. MF media (modified BG11 media) and additional ingredients such as aminolevulinic acid (2 mM), lysine (2.28 mM), alanine (1 mM), and Naphthalene acetic acid (1 mM) as cytokine promotedM. aeruginosagrowth and sugar contents.Salmonellashowed growth-assisting effect onM. aeruginosa. Enhanced growth rate and carbohydrates contents were observed inM. aeruginosaculture grown at 25°C under red LED light of 90 μmolm−2s−1intensity. More greenish and carbohydrates richM. aeruginosabiomass was prepared (final OD660 nm= 2.21 and sugar contents 10.39 mM/mL) as compared to control (maximum OD660 nm= 1.4 and sugar contents 3 mM/mL). The final algae biomass was converted to algae juice through a specific pretreatment method. The resulted algae Juice was used as a substrate in fermentation process. Highest yield of bioethanol (50 mM/mL) was detected whenBrettanomyces custersainus,Saccharomyces cerevisiae, andPichia stipitiswere used in combinations for fermentation process as compared to their individual fermentation. The results indicated the influence of different factors on the growth rate and carbohydrates productivity ofM. aeruginosaand its feasibility as a feedstock for fermentative ethanol production.

scholarly journals Design and Optimization of a Process for Sugarcane Molasses Fermentation bySaccharomyces cerevisiaeUsing Response Surface Methodology

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Nour Sh. El-Gendy ◽  
Hekmat R. Madian ◽  
Salem S. Abu Amr
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Batch Fermentation ◽  
Fermentation Process ◽  
Incubation Temperature ◽  
Operating Conditions ◽  
Quadratic Model ◽  
Optimum Operating ◽  
Sugarcane Molasses ◽  
Bioethanol Production

A statistical model was developed in this study to describe bioethanol production through a batch fermentation process of sugarcane molasses by locally isolatedSaccharomyces cerevisiaeY-39. Response surface methodology RSM based on central composite face centered design CCFD was employed to statistically evaluate and optimize the conditions for maximum bioethanol production and study the significance and interaction of incubation period, initial pH, incubation temperature, and molasses concentration on bioethanol yield. With the use of the developed quadratic model equation, a maximum ethanol production of 255 g/L was obtained in a batch fermentation process at optimum operating conditions of approximately 71 h, pH 5.6, 38°C, molasses concentration 18% wt.%, and 100 rpm.

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