A microbubble-sparged yeast propagation–fermentation process for bioethanol production

Microcystis aeruginosa, a freshwater microalga, is capable of producing and accumulating different types of sugars in its biomass which make it a good feedstock for bioethanol production. Present study aims to investigate the effect of different factors increasing growth rate and carbohydrates productivity ofM. aeruginosa. MF media (modified BG11 media) and additional ingredients such as aminolevulinic acid (2 mM), lysine (2.28 mM), alanine (1 mM), and Naphthalene acetic acid (1 mM) as cytokine promotedM. aeruginosagrowth and sugar contents.Salmonellashowed growth-assisting effect onM. aeruginosa. Enhanced growth rate and carbohydrates contents were observed inM. aeruginosaculture grown at 25°C under red LED light of 90 μmolm−2s−1intensity. More greenish and carbohydrates richM. aeruginosabiomass was prepared (final OD660 nm= 2.21 and sugar contents 10.39 mM/mL) as compared to control (maximum OD660 nm= 1.4 and sugar contents 3 mM/mL). The final algae biomass was converted to algae juice through a specific pretreatment method. The resulted algae Juice was used as a substrate in fermentation process. Highest yield of bioethanol (50 mM/mL) was detected whenBrettanomyces custersainus,Saccharomyces cerevisiae, andPichia stipitiswere used in combinations for fermentation process as compared to their individual fermentation. The results indicated the influence of different factors on the growth rate and carbohydrates productivity ofM. aeruginosaand its feasibility as a feedstock for fermentative ethanol production.

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Design and Optimization of a Process for Sugarcane Molasses Fermentation bySaccharomyces cerevisiaeUsing Response Surface Methodology

International Journal of Microbiology ◽

10.1155/2013/815631 ◽

2013 ◽

Vol 2013 ◽

pp. 1-9 ◽

Cited By ~ 24

Author(s):

Nour Sh. El-Gendy ◽

Hekmat R. Madian ◽

Salem S. Abu Amr

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Batch Fermentation ◽

Fermentation Process ◽

Incubation Temperature ◽

Operating Conditions ◽

Quadratic Model ◽

Optimum Operating ◽

Sugarcane Molasses ◽

Bioethanol Production

A statistical model was developed in this study to describe bioethanol production through a batch fermentation process of sugarcane molasses by locally isolatedSaccharomyces cerevisiaeY-39. Response surface methodology RSM based on central composite face centered design CCFD was employed to statistically evaluate and optimize the conditions for maximum bioethanol production and study the significance and interaction of incubation period, initial pH, incubation temperature, and molasses concentration on bioethanol yield. With the use of the developed quadratic model equation, a maximum ethanol production of 255 g/L was obtained in a batch fermentation process at optimum operating conditions of approximately 71 h, pH 5.6, 38°C, molasses concentration 18% wt.%, and 100 rpm.

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Optimization of Saccharification and Fermentation Process in Bioethanol Production from Oil Palm Fronds

Procedia Chemistry ◽

10.1016/j.proche.2015.12.002 ◽

2015 ◽

Vol 16 ◽

pp. 141-148 ◽

Cited By ~ 17

Author(s):

Eka Triwahyuni ◽

Sri Hariyanti ◽

Deliana Dahnum ◽

Muhammad Nurdin ◽

Haznan Abimanyu

Keyword(s):

Oil Palm ◽

Fermentation Process ◽

Bioethanol Production ◽

Oil Palm Fronds ◽

Palm Fronds

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Bioethanol Production from Enzymatic Hydrolyzates of Pretreated Flaxseed Meals by Baker’s Yeast

10.21203/rs.3.rs-599700/v1 ◽

2021 ◽

Author(s):

SAHELI GHOSAL ◽

JAYATI BHOWAL

Keyword(s):

Enzymatic Hydrolysis ◽

Fermentation Process ◽

Reducing Sugars ◽

Reducing Sugar ◽

Baker’S Yeast ◽

Baker's Yeast ◽

Bioethanol Production ◽

Dilute Acid ◽

Cellulose Conversion ◽

Flaxseed Meal

Abstract The present study investigated the usefulness of flaxseed meals as a novel feedstock for the production of bioethanol. The proximate composition of the flaxseed meal was carried out before the pretreatment of the flaxseed meal. In this study, flaxseed meal was pretreated with dilute acid, alkali, and aqueous for disruption of lignocellulosic compounds. The acid pretreated flaxseed meal was used for enzymatic hydrolysis by different enzymes (cellulase, α-amylase, and cellulase combined with α-amylase) for better release of reducing sugar. The cellulose conversion to reducing sugar was significantly higher for acid pretreated flaxseed meals. After enzymatic hydrolysis with cellulase, cellulose conversions to reducing sugars were found to be significantly higher than those of α-amylase and cellulase combined with α-amylase. The bioethanol production was also investigated. The fermentation process was carried out by using baker’s yeast (Saccharomyces cerevisiae) with the acid pretreated flaxseed meal enzymatic hydrolyzate. Maximum ethanol production (0.11 g/l) was achieved from the fermented medium obtained from the acid pretreated flaxseed meal followed by enzymatic hydrolysis by using cellulase enzyme. The structural analysis of bioethanol was also investigated by FTIR.

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Analisis Kualitas Bioetanol Dari Kulit Pisang

Jurnal Daur Lingkungan ◽

10.33087/daurling.v4i2.72 ◽

2021 ◽

Vol 4 (2) ◽

pp. 35

Author(s):

G M Saragih ◽

Hadrah Hadrah ◽

Dilla Tri Maharani

Keyword(s):

Fermentation Process ◽

Starch Content ◽

Soil Surface ◽

Raw Material ◽

Banana Peel ◽

Total Production ◽

Bioethanol Production ◽

Fermentation Time ◽

Banana Production

Indonesia is ranked sixth in banana production with total production in 2015 of 7.299.275. the more people who like bananas, the higher the volume of banana peel waste produced. Banana peels are usually thrown away immediately and can contaminate the soil surface because banana peels contain acid so that it can have an impact on evironmental problems. The starch content of banan peels has the potential to be used as a raw material for bioethanol production with the help of the fermentation process. Therefore this research aims to determine the quality of bioethanol which is produced from several types of banana peels. The types of banana peels used are Ambon banana peel, Barangan banana peel and horn banana peel. The method used to manufacture bioethanol form the types of banana peel of ambon, barangan and horn is fermentation using yeast tape or saccharomyses cereviciae. The variables observed in this study were the variety of banana peel types, fermentation time for 6 days and 10 days, and the use of yeast as much as 5 grams. The fermentation results in the form of bioethanol were analyzed using gas chromatography, the best results from this study were obtained on the type of banana peel of Ambon for 10 days, that is 4.451% bioethanol.

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