Development of Cryopreservation Procedure Using Vitrification Method of Cymbidium aloifolium (L.) Sw., Medicinal Orchid

2021 ◽  
Vol 21 (1) ◽  
pp. 21-30
Author(s):  
Attachai Trunjaruen ◽  
Pitakpong Maneeratta ◽  
Worasitikulya Taratima
Keyword(s):  
Cymbidium Aloifolium ◽  
Medicinal Orchid

scholarly journals Micropropagation of Cymbidium aloifolium (L.) SW., A Medicinal Orchid by Artificial Seeds Technology

2015 ◽  
Vol 28 ◽  
pp. 42-48 ◽  
Author(s):  
Shreeti Pradhan ◽  
Babu Lal Tiruwa ◽  
Bijay R Subedee ◽  
Bijaya Pant
Keyword(s):  
Basal Medium ◽  
Mass Scale ◽  
Scale Production ◽  
Ms Medium ◽  
Artificial Seeds ◽  
Seed Technology ◽  
Artificial Seed ◽  
Cymbidium Aloifolium ◽  
Medicinal Orchid

Artificial seed technology is a rapidly growing area of research in plant cell and tissue culture. Application of this technology opens an alternative route for mass scale production, efficient delivery of cloned plantlets and fulfils the increasing demand of local growers. An attempt was made to produce artificial seeds and their subsequent regeneration of a highly valuable medicinal orchid of Nepal i.e. Cymbidium aloifolium. Artificial seeds were obtained through encapsulation of protocorms in calcium alginate beads. Protocorms were encapsulated by using 3% sodium alginate and 0.2 M calcium chloride solution. Murashige and Skoog (MS) medium (1962) was used as the basal medium for in vitro germination and seedling development of artificial seed. In Cymbidium aloifolium, 20-25 days old in vitro grown protocorms were used for production of artificial seeds. Artificial seeds were inoculated on two different culture conditions of MS medium i.e. MS solid & MS liquid with four different treatments i.e. strength of 1.0, ½, ¼ and MS media supplemented with plant growth regulators viz. BAP (0.5 mg/l) and NAA (0.5 mg/l). Highest percentage of germination (100%) and plantlet conversion was found on hormone free full strength (1.0 MS) of MS liquid medium after 13-14 weeks of culture. Plantlets regenerated from artificial seeds with well developed shoot and root systems were successfully acclimatized in potting mixture of cocopeat, litter and sphagnum moss in a ratio 2:1:1.J. Nat. Hist. Mus. Vol. 28, 2014: 42-48

scholarly journals Dual Phase Regeneration System for Mass Propagation of Cymbidium aloifolium (L.) Sw.: A High Value Medicinal Orchid

2019 ◽  
Vol 29 (2) ◽  
pp. 257-266
Author(s):  
Mohammad Musharof Hossain ◽  
Madhu Sharma
Keyword(s):  
Root Systems ◽  
Dual Phase ◽  
Mass Propagation ◽  
Regeneration System ◽  
Prolonged Culture ◽  
Half Strength ◽  
Cymbidium Aloifolium ◽  
Medicinal Orchid ◽  
Stem Segments

Efficient micropropagation protocol was established in Cymbidium aloifolium through induction of secondary (2°) protocorms from primary (1°) protocorms, shoot buds (SBs) and protocorm-like bodies (PLBs) from pseudo-stem segments of in vitro raised seedlings. Neo-formation of 2° protocorms from 1° protocorms was obtained in liquid and agar-solidified Phytamax (PM) medium with NAA, 2,4-D, BAP and Kn at different concentrations and combinations. The highest number of 2° protocorms was induced in liquid PM medium (9.76 ± 0.88/1° protocorm) amended with 2.0 mg/l BAP + 1.0 mg/l 2,4- D. Although protocorms proliferated profusely in liquid medium, hyperhydricity was observed after prolonged culture. Both SBs and PLBs were induced from pseudo-stem segments on agar gelled PM medium and the mode of differentiation was dependent upon specific PGRs concentrations. The maximum number of SBs (5.80 ± 2.59/explant) was recorded in BAP and NAA at 1.0 mg/l each, while the maximum number of PLBs (7.20 ± 3.22/explant) were induced in 2.0 mg/l BAP and NAA each. Well developed root systems were induced SBs and PLBs on transferring to half-strength PM medium fortified with 0.5 mg/l IAA. The rooted plantlets were transferred to the greenhouse with 95% survival.

scholarly journals In vitro germination and propagation of a threatened medicinal orchid, Cymbidium aloifolium (L.) Sw. through artificial seed

2014 ◽  
Vol 4 (12) ◽  
pp. 971-976 ◽  
Author(s):  
Shreeti Pradhan ◽  
Babulal Tiruwa ◽  
Bijay Raj Subedee ◽  
Bijaya Pant
Keyword(s):  
In Vitro Germination ◽  
Artificial Seed ◽  
Cymbidium Aloifolium ◽  
Medicinal Orchid

scholarly journals Detection and Characterization of Antibacterial Siderophores Secreted by Endophytic Fungi from Cymbidium aloifolium

Biomolecules ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1412
Author(s):  
Srinivas Chowdappa ◽  
Shubha Jagannath ◽  
Narasimhamurthy Konappa ◽  
Arakere C. Udayashankar ◽  
Sudisha Jogaiah
Keyword(s):  
Endophytic Fungi ◽  
Plant Pathogens ◽  
Charge Ratio ◽  
Total Protein Content ◽  
Exact Mass ◽  
Spectra Analysis ◽  
Development Of Resistance ◽  
Cymbidium Aloifolium ◽  
Medicinal Orchid ◽  
Blight Disease

Endophytic fungi from orchid plants are reported to secrete secondary metabolites which include bioactive antimicrobial siderophores. In this study endophytic fungi capable of secreting siderophores were isolated from Cymbidium aloifolium, a medicinal orchid plant. The isolated extracellular siderophores from orchidaceous fungi act as chelating agents forming soluble complexes with Fe3+. The 60% endophytic fungi of Cymbidium aloifolium produced hydroxamate siderophore on CAS agar. The highest siderophore percentage was 57% in Penicillium chrysogenum (CAL1), 49% in Aspergillus sydowii (CAR12), 46% in Aspergillus terreus (CAR14) by CAS liquid assay. The optimum culture parameters for siderophore production were 30 °C, pH 6.5, maltose and ammonium nitrate and the highest resulting siderophore content was 73% in P. chrysogenum. The total protein content of solvent-purified siderophore increased four-fold compared with crude filtrate. The percent Fe3+ scavenged was detected by atomic absorption spectra analysis and the highest scavenging value was 83% by P. chrysogenum. Thin layer chromatography of purified P. chrysogenum siderophore showed a wine-colored spot with Rf value of 0.54. HPLC peaks with Rts of 10.5 and 12.5 min were obtained for iron-free and iron-bound P. chrysogenum siderophore, respectively. The iron-free P. chrysogenum siderophore revealed an exact mass-to-charge ratio (m/z) of 400.46 and iron-bound P. chrysogenum siderophore revealed a m/z of 453.35. The solvent-extracted siderophores inhibited the virulent plant pathogens Ralstonia solanacearum, that causes bacterial wilt in groundnut and Xanthomonas oryzae pv. oryzae which causes bacterial blight disease in rice. Thus, bioactive siderophore-producing endophytic P. chrysogenum can be exploited in the form of formulations for development of resistance against other phytopathogens in crop plants.

scholarly journals In vitro seed germination of Cymbidium aloifolium (L.) Sw., a potential medicinal Orchid from Eastern Ghats of Tamil Nadu, India

2017 ◽  
Vol 44 (3) ◽  
pp. 343-348 ◽  
Author(s):  
Philip Robinson ◽  
J. ◽  
Jyoti Prasad Kakati ◽  
Sebastinraj ◽  
J. ◽  
...  
Keyword(s):  
Seed Germination ◽  
Tamil Nadu ◽  
Eastern Ghats ◽  
Cymbidium Aloifolium ◽  
Medicinal Orchid

Cost-Effective in vitro Multiplication and Phenolic Profile of an Important Medicinal Orchid, Satyrium nepalense D. Don

2021 ◽  
Vol 11 (2) ◽  
pp. 162-182
Author(s):  
Deepak Kumar Singh ◽  
Bilal Ahmad Mir ◽  
Sadhana Babbar ◽  
Shashi Bhushan Babbar
Keyword(s):  
Cost Effective ◽  
Phenolic Profile ◽  
In Vitro Multiplication ◽  
Medicinal Orchid

scholarly journals Ecological analysis and environmental niche modelling of Dactylorhiza hatagirea (D. Don) Soo: A conservation approach for critically endangered medicinal orchid

2021 ◽  
Author(s):  
Ishfaq Ahmad Wani ◽  
Susheel Verma ◽  
Shazia Mushtaq ◽  
Abdulaziz Abdullah Alsahli ◽  
Mohammed Nasser Alyemeni ◽  
...  
Keyword(s):  
Critically Endangered ◽  
Environmental Niche ◽  
Niche Modelling ◽  
Ecological Analysis ◽  
Medicinal Orchid

Assessment of clonal fidelity and phytomedicinal potential in micropropagated plants of Bulbophyllum odoratissimum - An endangered medicinal orchid of Indo Burma megabiodiversity hotspot

2021 ◽  
Vol 141 ◽  
pp. 487-497
Author(s):  
Gargi Prasad ◽  
Tapan Seal ◽  
Ashiho A. Mao ◽  
Deepu Vijayan ◽  
Adani Lokho
Keyword(s):  
Clonal Fidelity ◽  
Micropropagated Plants ◽  
Medicinal Orchid
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