Evaluation of Natural Exudate Gum from Sterculia urens as Gelling Agent in Culture Media for In vitro Regeneration of Rough Lemon (Citrus jambhiri Lush.) Shoot Tips

The effect of Murashige and Skoog (MS) medium supplemented with various concentrations and combinations of growth hormones on direct regeneration from one month old epicotyl segments of in vitro grown rough lemon (Citrus jambhiri Lush.) seedlings was studied. The earliest bud induction in 7.5 days, highest bud induction frequency (98.50%), percent regeneration(90.53) were obtained on MS medium supplemented with 6-Benzylaminopurine (BAP) (1mglit-1) with an average number of 12.50 buds per explants. The epicotyls segments with proliferated buds were transferred to elongation media in order to improve the recovery of normal shoots. Maximum number of elongated shoots (8.50) was obtained on MS medium having BAP (0.5mglit-1) + Gibberellic Acid (GA3)(1.0 mglit-1).These elongated shoots were then rooted on MS medium containing Indole-3-butyric acid (IBA) (0.1mglit-1) + Indole-3-aceticacid(IAA)(0.5mglit-1) with highest rooting percentage(96%) and root number(5.0). Early (10.10 days) rooting was observed in MS medium supplemented with NAA1.0 mglit-1 + IBA0.5 mglit-1.The plantlet survival was 98.52%, when plantlets were transferred to plastic pots containing a mixture of garden soil and vermiculite (1:1). The hardened plants were successfully established in the soil. The present study developed protocol which can be reliably used for in vitro regeneration of rough lemon and for gene transfer studies in rough lemon, especially to induce salinity and Phytophthora tolerance.

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CLONAL PROPAGATION OF NEEM (Azadirachta indica A. Juss.) VIA DIRECT AND INDIRECT IN VITRO REGENERATION

Revista Árvore ◽

10.1590/0100-67622015000300004 ◽

2015 ◽

Vol 39 (3) ◽

pp. 439-445 ◽

Cited By ~ 4

Author(s):

Laureen Michelle Houllou ◽

Robson Antônio de Souza ◽

Elizabete Cristina Pacheco dos Santos ◽

José Jackson Pereira da Silva ◽

Marta Ribeiro Barbosa ◽

...

Keyword(s):

Azadirachta Indica ◽

Callus Formation ◽

In Vitro Regeneration ◽

Regenerative Process ◽

Shoot Tips ◽

Shoot Tip ◽

Stem Base ◽

Different Characteristics

ABSTRACTThe study was conducted with shoot tip explants of neem (Azadirachta indica A. Juss) to identify a viable regenerative process. Shoot tips were obtained from neem embryos cultured alternatingly in DKW medium supplemented with BAP and medium without hormones. Initial shoot development was influenced by cotyledon presence. Basal callus, excised from in vitro stem base, also presented organogenic potential. In some cases, plant lines, obtained from each seed, presented different characteristics. The most common characteristic observed in vitro was callus formation at the stem base. However, the rarest characteristics were stem callus formation and leaf senescence. The regenerated shoot tips were further subculture and rooted on a medium supplemented with IBA so that complete plants could be obtained. The rooted plants were transplanted to a greenhouse and successfully acclimatized. No significant differences in in vivo development were observed between neem plants from callus and from shoot tip propagation.

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Types and concentrations of cytokinins in the micropropagation of Anthurium maricense

Revista Agro mbiente On-line ◽

10.18227/1982-8470ragro.v12i2.4671 ◽

2018 ◽

Vol 12 (2) ◽

pp. 117

Author(s):

Cecília Moreira Serafim ◽

Arlene Santisteban Campos ◽

Priscila Bezerra Dos Santos Melo ◽

Ana Cecília Ribeiro de Castro ◽

Ana Cristina Portugal Pinto de Carvalho

Keyword(s):

Light Intensity ◽

Culture Medium ◽

Culture Media ◽

In Vitro Regeneration ◽

Nodal Segments ◽

Nodal Segment ◽

Viable Option ◽

Growth Room ◽

In Vitro Induction

Faced with the demand for plants potted for their foliage, Anthurium maricense is seen as a viable option. However, most of the studies on obtaining micropropagated plantlets are for A. andraeanum, with nothing yet reported for A. maricense. The aim of this study therefore, was to evaluate the effect of four cytokinins in different concentrations, on the in vitro induction of shoots from nodal segments of A. maricense. The experimental design was completely randomised in a 4 x 4 factorial scheme, with four cytokinins (BAP, ZEA, CIN and 2iP) and 4 concentrations (0, 2.22, 4.44 and 6.66 μM), for a total of 16 treatments, with 6 replications of five test tubes, and using one nodal segment. Cultures were kept in a growth room at 25 ± 2°C, a photoperiod of 16 h and a light intensity of 30 μmolm-2 s-1 for 60 days. After this period, the number of shoots formed per node was evaluated. The addition of a cytokinin to the culture medium was determinant for the in vitro regeneration of shoots in A. maricense. The greatest estimated number of shoot formations in A. maricense were obtained in the culture media containing ZEA (3.87) and BAP (3.30), both at concentration of 6.66 μM.

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Evaluation of the Enhanced Regeneration System for in vitro regeneration in barley

Canadian Journal of Plant Science ◽

10.4141/p05-055 ◽

2006 ◽

Vol 86 (1) ◽

pp. 63-69

Author(s):

Seedhabadee Ganeshan ◽

Brian J Weir ◽

Monica Båga ◽

Brian G Rossnagel ◽

Ravindra N Chibbar

Keyword(s):

Hordeum Vulgare ◽

Embryogenic Callus ◽

Culture Media ◽

In Vitro Regeneration ◽

Cold Treatment ◽

Regeneration System ◽

Embryogenic Calli ◽

Hordeum Vulgare L ◽

Best Response

A simple two-step model for evaluation of in vitro regeneration protocols is proposed based on callus induction and regeneration from immature scutella of two Canadian barley (Hordeum vulgare L.) genotypes, AC Metcalfe and SB92559 using the Enhanced Regeneration System (ERS). The number of explants producing embryogenic callus, the number of plants per embryogenic callus and the number of plants per explant were considered. Tissue culture parameters included three combinations of growth regulators, two carbon sources in culture media, and three cold treatment regimes of spikes prior to scutella isolation. Culture medium containing 5 µM 2,4-dichlorophenoxy acetic acid (2,4-D) and 0.5 µM benzyl adenine (BA) induced the highest percent of embryogenic calli and the highest number of shoots per embryogenic callus from AC Metcalfe. Medium containing 3.75 µM 2,4-D and 0.75 µM BA gave the best response for SB92559. Both genotypes produced more shoots on maltose than on sucrose medium. A 2-d treatment of spikes at 4°C resulted in best response for SB92559. Regeneration response from AC Metcalfe scutella from spikes was unaffected by being subjected to 2, 4 or 6 d of cold. Conditions resulting in best responses from both genotypes were tested on four commercial barley varieties. However, these lines showed inferior regeneration compared to SB92559 and AC Metcalfe. Key words: Hordeum vulgare, scutella, embryogenic callus, shoot production

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Evaluation of rough lemon (Citrus jambhiri Lush.) as rootstock for salinity tolerance at seedling stage under in vitro conditions

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb2013.12994 ◽

2013 ◽

Vol 12 (44) ◽

pp. 6267-6275 ◽

Cited By ~ 4

Author(s):

K Sharma L ◽

Kaushal Manisha ◽

Kaur Bali Sukhwinder ◽

P Choudhary O

Keyword(s):

Salinity Tolerance ◽

Seedling Stage ◽

Citrus Jambhiri ◽

Rough Lemon

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In vitro Regeneration and Callogenesis of Libidibia ferrea

Journal of Experimental Agriculture International ◽

10.9734/jeai/2020/v42i430495 ◽

2020 ◽

pp. 14-24

Author(s):

Daniel da Silva ◽

Angela Maria Imakawa ◽

Kamylla Rosas Vieira Guedes ◽

Flávio Mauro Souza Bruno ◽

Paulo de Tarso Barbosa Sampaio

Keyword(s):

Culture Media ◽

In Vitro Regeneration ◽

Shoot Multiplication ◽

Light Sources ◽

Multiplication Rate ◽

Medicinal Species ◽

Efficient System ◽

Blue Led ◽

Friable Callus

Libidibia ferrea (Fabaceae) is a valuable medicinal species in the Amazon, but as it is a protected plant, collection from natural populations is forbidden. Therefore, establishing an efficient system for in vitro regeneration and to improve callogenesis of this species is desirable. To determine the optimal nutritional factors needed for shoot multiplication and callus induction, different culture media, plant growth regulators and LED light sources were tested. The data were subjected to analysis of variance (ANOVA) and means compared by Tukey’s test at p < 0.05. We observe that explants inoculated in the Murashige and Skoog (MS) media with 0.05 mg L-1 of 6-benzilaminopurine (BAP) and cultivated under red-blue LED induced the highest number of shoots (3.67), number of buds (3.13), multiplication rate (15.67) and shoots length (22.03 mm) when compared with other treatments. MS and B5 media supplemented with 2.21 and 4.42 mg L-1 of 2,4-D induced 100% formation of friable callus cultivated under red-blue LED, demonstrating that the light quality significantly influenced callogenesis. Obtained results confirmed that in vitro regeneration and callogenesis is a useful strategy in the protection of endangered species. In this way, a new renewable source of biomass with high quality plant material is presented aiming at the bioprospecting of seedling extracts and friable callus to obtain secondary metabolites of this medicinal plant.

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Influence of Additional Nutrients and Gelling Agents on in Vitro Response of Selected Indica Rice Varieties

International Journal of Biology ◽

10.5539/ijb.v11n4p26 ◽

2019 ◽

Vol 11 (4) ◽

pp. 26

Author(s):

Sai Krishna Repalli ◽

Chaitanya Kumar Geda ◽

N. S. N. Pradhan ◽

G. J. N. Rao

Keyword(s):

Culture Media ◽

Indica Rice ◽

Casein Hydrolysate ◽

Gelling Agent ◽

Regeneration Potential ◽

Rice Varieties ◽

Gelling Agents ◽

Profound Influence ◽

In Vitro Response

Indica rice varieties are recalcitrant to culture and hence the culture media should be supplemented with additional nutrients to provide energy and osmotic potential for best in vitro response. Combinations of plant growth regulators have profound influence on callus induction and regeneration potential of the selected genotypes. In addition, concentration and choice of gelling agents also have their effect on regeneration of indica rice varieties. Impact of L-Proline, and Casein Hydrolysate on tissue culture response of selected indica rice varieties is discussed and the best choice of gelling agent and their in vitro response is elucidated.

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Long-term in Vitro Regeneration of Hamelia patens

HortScience ◽

10.21273/hortsci.30.4.873g ◽

1995 ◽

Vol 30 (4) ◽

pp. 873G-874

Author(s):

D. Sankhla ◽

T.D. Davis ◽

N. Sankhla ◽

A. Upadhyaya

Keyword(s):

Culture Medium ◽

In Vitro Regeneration ◽

Shoot Formation ◽

Shoot Tips ◽

Shoot Cultures ◽

Ms Medium ◽

Prolific Shoot ◽

Ornamental Shrub

This report describes an efficient in vitro regeneration protocol for H. patens (firebush), a heat-tolerant ornamental shrub native to tropical and subtropical America. Shoot cultures were initially established using shoot tips placed on MS-revised medium containing 2.3 μM 2,4-D, 2.3 μM kinetin, and 0.25% polyvinylpyrrolidone. Other types of explants (nodal and internodal segments, leaf pieces, floral buds) did not regenerate shoots when placed on this medium. Two-month-old plantlets derived from the shoot tips were subcultured on MS medium supplemented with 0.5 μM thidiazuron (TDZ), and within 3 to 4 weeks, some callus was produced at the root–shoot junction. When this callus, with a small portion of the root and shoots, was placed on MS medium with 0.05 μM TDZ and 0.01 μM ABA, prolific shoot formation occurred within 3 to 4 weeks followed by root formation. By regular subculturing every 5 to 6 weeks, hundreds of plantlets have been obtained over the past 3 years with no apparent decline in regeneration potential. Addition of activated charcoal (0.5%) to the culture medium has greatly improved growth of the plantlets.

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