Comparison of agar and gum karaya as gelling agent for in vitro regeneration of rough lemon (Citrus jambhiri Lush.) plantlets from nodal explants

The effect of Murashige and Skoog (MS) medium supplemented with various concentrations and combinations of growth hormones on direct regeneration from one month old epicotyl segments of in vitro grown rough lemon (Citrus jambhiri Lush.) seedlings was studied. The earliest bud induction in 7.5 days, highest bud induction frequency (98.50%), percent regeneration(90.53) were obtained on MS medium supplemented with 6-Benzylaminopurine (BAP) (1mglit-1) with an average number of 12.50 buds per explants. The epicotyls segments with proliferated buds were transferred to elongation media in order to improve the recovery of normal shoots. Maximum number of elongated shoots (8.50) was obtained on MS medium having BAP (0.5mglit-1) + Gibberellic Acid (GA3)(1.0 mglit-1).These elongated shoots were then rooted on MS medium containing Indole-3-butyric acid (IBA) (0.1mglit-1) + Indole-3-aceticacid(IAA)(0.5mglit-1) with highest rooting percentage(96%) and root number(5.0). Early (10.10 days) rooting was observed in MS medium supplemented with NAA1.0 mglit-1 + IBA0.5 mglit-1.The plantlet survival was 98.52%, when plantlets were transferred to plastic pots containing a mixture of garden soil and vermiculite (1:1). The hardened plants were successfully established in the soil. The present study developed protocol which can be reliably used for in vitro regeneration of rough lemon and for gene transfer studies in rough lemon, especially to induce salinity and Phytophthora tolerance.

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In vitro regeneration and induction of multiple shooting in Cicer arietinum L. using cotyledonary nodal explants

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb2013.13547 ◽

2015 ◽

Vol 14 (13) ◽

pp. 1129-1138 ◽

Cited By ~ 4

Author(s):

Prema Sunil Sruthi ◽

Philip Robinson J ◽

S KarthickBalan S ◽

Anandhaprabhakaran M ◽

Balakrishnan V

Keyword(s):

Cicer Arietinum ◽

In Vitro Regeneration ◽

Nodal Explants ◽

Multiple Shooting ◽

Cicer Arietinum L

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Assessment of In-vitro culture through Nodal explants of Dendrocalamus hamiltonii

International Journal of Agricultural and Applied Sciences ◽

10.52804/ijaas2021.2115 ◽

2021 ◽

Vol 2 (1) ◽

pp. 130-133

Author(s):

Abha Jha ◽

◽

Sunila Das ◽

Keyword(s):

Experimental Study ◽

Growth Regulators ◽

In Vitro Regeneration ◽

Shoot Multiplication ◽

Growth Hormones ◽

Nodal Explants ◽

Bud Break ◽

Multiplication Rate ◽

Dendrocalamus Hamiltonii

The present experimental study was aimed to overcome the traditional methods of propagation that limit the number of propagules by in-vitro regeneration through nodal explants of Dendrocalamus hamiltonii with a comparative study of growth regulators during the shooting and rooting process. Dendrocalamus hamiltonii is distributed from the Himalayas (Nepal) to the northern part of Burma. Collection of explants was done from different selected sites of CPTs. There was the use of HgCl2 and Ca (OCl)2 as sterilizing agents in different concentrations and its effect was visualized during the sprouting stage. Culm explants were cultured in a bottle containing White media (Wm) supplemented with BA and Kinetin for sprouting and IAA, IBA, NAA for rooting. There is also the use of IAA+IBA+NAA in combined form as a supplementary solution 0.1% HgCl2 treatment for 20-minute results into77.80% aseptic buds and 72% bud -break. Among the used growth-hormones, BA with concentration 0.25mg/l and 0.50mg/l respectively were appropriate for shoot-multiplication rate, 4.01±0.3 and 4.3±0.4 were ideal observation incorporation with BA (1.00mg/l) and BA (1.50mg/l) respectively. Maximum sprouting rate14.77±3.37with application of BA (2.00mg/l) and maximum shoot length4.3±0.4 is observed at BA (1.50mg/l). The applications of rooting hormone IAA+IBA+NAA in the concentration of 1.0 mg/l results in 72.5±0.3(rooting) and 11.1±0.3 (av. No. of the root).

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In vitro Regeneration of Plantlets from Leaf and Nodal explants of Aristolochia indica L.— An Important Threatened Medicinal Plant

Asian Pacific Journal of Tropical Biomedicine ◽

10.1016/s2221-1691(12)60259-7 ◽

2012 ◽

Vol 2 (2) ◽

pp. S488-S493 ◽

Cited By ~ 3

Author(s):

Pramod V. Pattar ◽

M. Jayaraj

Keyword(s):

Medicinal Plant ◽

In Vitro Regeneration ◽

Nodal Explants ◽

Threatened Medicinal Plant

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In vitro propagation of Citrullus lanatus Thumb. from nodal explants culture

Journal of the Bangladesh Agricultural University ◽

10.3329/jbau.v8i2.7926 ◽

1970 ◽

Vol 8 (2) ◽

pp. 203-206 ◽

Cited By ~ 1

Author(s):

MM Khatun ◽

MS Hossain ◽

MA Haque ◽

M Khalekuzzaman

Keyword(s):

Citrullus Lanatus ◽

In Vitro Regeneration ◽

Shoot Proliferation ◽

Multiple Shoot ◽

Nodal Explants ◽

Root Induction ◽

Ms Medium ◽

Nodal Explant ◽

Grown Plant

A standard protocol was established for rapid in vitro propagation of watermelon (Citrullus lanatus Thumb.) from nodal explants of field grown plant. Multiple shoot proliferation was achieved from nodal explants on MS medium supplemented with 1.0 mg/l BAP + 0.2 mg/l NAA within 30 days of inoculation. The elongation of shoots was obtained on the same medium. Highest percentage of root induction was achieved on MS medium supplement with 1.0 mg/l IBA within 25 days of culture. Well rooted plantlets were transferred to small pots and after proper acclimatization the plantlets were transplanted in the field condition, where 80% plantlets were survived and grew successfully. Keywords: In vitro regeneration; Nodal explant; Citrullus lanatus DOI: 10.3329/jbau.v8i2.7926 J. Bangladesh Agril. Univ. 8(2): 203-206, 2010

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