Effects of Genotype on Induction of Callus and Plant Regeneration Potential in vitro Anther Culture of Rice (Oryza sativa L.)

2004 ◽  
Vol 7 (2) ◽  
pp. 235-237 ◽  
Author(s):  
Sharmin Shahnewaz ◽  
M.A. Bari . ◽  
N.A. Siddique . ◽  
M.H. Rahman .
Keyword(s):  
Oryza Sativa ◽  
Plant Regeneration ◽  
Anther Culture ◽  
Oryza Sativa L ◽  
Regeneration Potential

In vitro Plant Regeneration Through Anther Culture of Some Iranian Local Rice (Oryza sativa L.) Cultivars

2007 ◽  
Vol 10 (12) ◽  
pp. 2056-2060 ◽  
Author(s):  
Reza Talebi . ◽  
Mohammad Reza Rahemi . ◽  
Habibolah Arefi . ◽  
Mohammad Nourozi . ◽  
Nadali Bagheri .
Keyword(s):  
Oryza Sativa ◽  
Plant Regeneration ◽  
Anther Culture ◽  
Oryza Sativa L ◽  
In Vitro Plant Regeneration ◽  
In Vitro Plant

Utilisation of anther culture in rice (Oryza sativa L.) breeding in Italy

1994 ◽  
Vol 34 (7) ◽  
pp. 911 ◽  
Author(s):  
AM Callegarin ◽  
K Perfanov ◽  
G Dorotea ◽  
G Baldi
Keyword(s):  
Oryza Sativa ◽  
Anther Culture ◽  
Grain Quality ◽  
Agronomic Traits ◽  
Oryza Sativa L ◽  
Market Demand ◽  
Breeding Methods ◽  
Rice Varieties ◽  
Hybrid Lines

Increasing market demand has led to efforts to select for non-sticky, long-grain rice varieties suited to the Italian environment. The variability of recombinant populations derived from crosses between Italian and USA genotypes was exploited. Together with the conventional breeding methods, in vitro anther culture was used to obtain homozygous lines from crosses more quickly. Moreover, hybrid lines were obtained from crosses otherwise impossible to exploit owing to sterility in the F, generation (namely those resulting from crosses involving semi-dwarf USA varieties). Plant regeneration was obtained from 6 F, hybrids whose parents were chosen for their grain quality or their suitability to the Italian environment. About 5500 plantlets were regenerated. Of these, 1000 were albino, and about 50% of the green ones died after transplanting into soil; 65% of the surviving plants were sterile, so about 1000 progeny were obtained (30-445 for each cross). Progeny with desirable characteristics were evaluated for agronomic traits. On the basis of these results, limits and possibilities of anther-culture breeding in rice are discussed.

scholarly journals Cyto-histological aspects of haploid androgenesis when obtaining haploids/doubled haploids in rice (Oryza Sativa l.) anther culture in vitro

2021 ◽  
Vol 285 ◽  
pp. 02033
Author(s):  
E. G. Savenko ◽  
Zh. M. Mukhina ◽  
V. A. Glazyrina ◽  
L. A. Shundrina
Keyword(s):  
Oryza Sativa ◽  
Anther Culture ◽  
Oryza Sativa L ◽  
Blast Resistance ◽  
Histological Study ◽  
Doubled Haploids ◽  
Abnormal Development ◽  
Characteristic Functions ◽  
Stage Of Development

The aim of the study was to study the development of rice microspores in anther culture in vitro, study the structure of androclinic callus to identify cyto-embryological features of the formation of morphogenic structures in anther culture, obtain doubled rice haploids (Oryza sativa L.) and accelerate the development of valuable breeding material with desired properties. Within the framework of this study, the results of a cyto-histological study of rice haploid androgenesis in vitro were obtained, which indicate that it is induced under the influence of phytohormones from rice anther microspores at the mononuclear or early binuclear stage of development. The abnormal development of microspores on nutrient media with phytohormone 2,4-D was traced, in which nuclei, having lost their characteristic functions, acquired the ability to unlimited division and growth with the formation of microcallus. The morphological structure of calli was assessed. The main morphotypes of callus tissues and the pathways of morphogenesis, leading to the formation of androgenic structures, up to full-fledged regenerant plants, were identified. Homozygous androgenic lines based on F1 and BC1 - rice generations obtained in the course of hybridization and backcrossing between Chinese samples carrying blast resistance genes and Russian accessiond were rapidly developed.

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