Callus Induction and in vitro Plant Regeneration of Rice (Oryza sativa L.) Under Various Conditions

2008 ◽  
Vol 11 (2) ◽  
pp. 255-259 ◽  
Author(s):  
Muhammad Tariq ◽  
Gowher Ali ◽  
Fazal Hadi ◽  
Shakeel Ahmad ◽  
Nasir Ali ◽  
...  
2007 ◽  
Vol 10 (12) ◽  
pp. 2056-2060 ◽  
Author(s):  
Reza Talebi . ◽  
Mohammad Reza Rahemi . ◽  
Habibolah Arefi . ◽  
Mohammad Nourozi . ◽  
Nadali Bagheri .

2019 ◽  
Vol 20 (9) ◽  
pp. 2079 ◽  
Author(s):  
Brenda A. López-Ruiz ◽  
Vasti T. Juárez-González ◽  
Estela Sandoval-Zapotitla ◽  
Tzvetanka D. Dinkova

In vitro plant regeneration addresses basic questions of molecular reprogramming in the absence of embryonic positional cues. The process is highly dependent on the genotype and explant characteristics. However, the regulatory mechanisms operating during organ differentiation from in vitro cultures remain largely unknown. Recently, miRNAs have emerged as key regulators during embryogenic callus induction, plant differentiation, auxin responses and totipotency. Here, we explored how development-related miRNA switches the impact on their target regulation depending on physiological and molecular events taking place during maize Tuxpeño VS-535 in vitro plant regeneration. Three callus types with distinctive regeneration potential were characterized by microscopy and histological preparations. The embryogenic calli (EC) showed higher miRNA levels than non-embryogenic tissues (NEC). An inverse correlation for miR160 and miR166 targets was found during EC callus induction, whereas miR156, miR164 and miR394 displayed similar to their targets RNA accumulation levels. Most miRNA accumulation switches took place early at regenerative spots coincident with shoot apical meristem (SAM) establishment, whereas miR156, miR160 and miR166 increased at further differentiation stages. Our data uncover particular miRNA-mediated regulation operating for maize embryogenic tissues, supporting their regulatory role in early SAM establishment and basipetala growth during the in vitro regeneration process.


2018 ◽  
Vol 45 (3) ◽  
pp. 266-272 ◽  
Author(s):  
Min Wan Park ◽  
Shi Hyun Ryu ◽  
Yoon Young Lee ◽  
Jae-Mo Song ◽  
Jin Ho Kim ◽  
...  

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