scholarly journals Molecular Cloning and Functional Expression of Atlantic Salmon Peptide Transporter 1 in Xenopus Oocytes Reveals Efficient Intestinal Uptake of Lysine-Containing and Other Bioactive Di- and Tripeptides in Teleost Fish

2010 ◽  
Vol 140 (5) ◽  
pp. 893-900 ◽  
Author(s):  
Ivar Rønnestad ◽  
Koji Murashita ◽  
Gabor Kottra ◽  
Ann-Elise Jordal ◽  
Shailesh Narawane ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
Molecular Cloning ◽  
Teleost Fish ◽  
Xenopus Oocytes ◽  
Functional Expression ◽  
Peptide Transporter ◽  
Intestinal Uptake ◽  
Peptide Transporter 1

Molecular cloning and functional expression of cDNAs encoding a human Na+-nucleoside cotransporter (hCNT1)

1997 ◽  
Vol 272 (2) ◽  
pp. C707-C714 ◽  
Author(s):  
M. W. Ritzel ◽  
S. Y. Yao ◽  
M. Y. Huang ◽  
J. F. Elliott ◽  
C. E. Cass ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Xenopus Oocytes ◽  
Polymerase Chain Reaction Amplification ◽  
Human Kidney ◽  
Functional Expression ◽  
Nucleoside Transporter ◽  
Renal Secretion ◽  
Transporter Protein ◽  
Reaction Amplification ◽  
Nucleoside Drugs

We report identification of a new human nucleoside transporter protein by molecular cloning and functional expression of its cDNA. Previously, we used expression selection in Xenopus oocytes to isolate a cDNA from rat jejunal epithelium encoding the pyrimidine-selective Na+-dependent nucleoside transporter rCNT1 (Q.-Q. Huang, S. Y. M. Yao, M. W. L. Ritzel, A. R. P. Paterson, C. E. Cass, and J. D. Young. J. Biol. Chem. 269: 17757-17760, 1994). cDNAs for a human homologue of rCNT1, designated hCNT1, have been isolated from human kidney by hybridization cloning and reverse transcriptase polymerase chain reaction amplification strategies. hCNT1 was 83% identical to rCNT1 in amino acid sequence and exhibited the transport characteristics of an Na+-dependent nucleoside transporter with selectivity for pyrimidine nucleosides and adenosine when expressed in Xenopus oocytes. Deoxyadenosine, which undergoes net renal secretion, and guanosine were poor permeants. hCNT1 did, however, transport 3'-azido-3'-deoxythymidine. This is the first demonstration that members of the CNT family exist in human cells and provides evidence of their involvement in the renal transport of physiological nucleosides and nucleoside drugs. The hCNT1 gene was mapped to chromosome 15q25-26.

scholarly journals Diabetes downregulates peptide transporter 1 in the rat jejunum: possible involvement of cholate-induced FXR activation

2020 ◽  
Vol 41 (11) ◽  
pp. 1465-1475
Author(s):  
Li-min Liang ◽  
Jun-jie Zhou ◽  
Feng Xu ◽  
Pei-hua Liu ◽  
Lan Qin ◽  
...  
Keyword(s):  
Peptide Transporter ◽  
Rat Jejunum ◽  
Peptide Transporter 1

scholarly journals Purification, Molecular Cloning, and Functional Expression of the Human Nicodinamide-Adenine Dinucleotide Phosphate-Regulated Thyroid Hormone-Binding Protein

1997 ◽  
Vol 11 (11) ◽  
pp. 1728-1736 ◽  
Author(s):  
Marie-Pierre Vié ◽  
Claudine Evrard ◽  
Jeannine Osty ◽  
Aline Breton-Gilet ◽  
Pascal Blanchet ◽  
...  
Keyword(s):  
Thyroid Hormone ◽  
Molecular Cloning ◽  
Binding Protein ◽  
Functional Expression ◽  
Hormone Binding ◽  
Hormone Binding Protein

Oseltamivir (Tamiflu) Is a Substrate of Peptide Transporter 1

2009 ◽  
Vol 37 (8) ◽  
pp. 1676-1681 ◽  
Author(s):  
Takuo Ogihara ◽  
Takashi Kano ◽  
Tamae Wagatsuma ◽  
Sho Wada ◽  
Hikaru Yabuuchi ◽  
...  
Keyword(s):  
Peptide Transporter ◽  
Peptide Transporter 1

scholarly journals Molecular cloning, sequencing, and functional expression of a cDNA encoding human coproporphyrinogen oxidase.

1994 ◽  
Vol 91 (8) ◽  
pp. 3024-3028 ◽  
Author(s):  
P. Martasek ◽  
J. M. Camadro ◽  
M. H. Delfau-Larue ◽  
J. B. Dumas ◽  
J. J. Montagne ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Functional Expression ◽  
Coproporphyrinogen Oxidase
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