Monitoring Bacterial Population Dynamics Using Real-Time PCR During the Bioremediation of Crude-Oil-Contaminated Soil

2009 ◽  
Vol 19 (4) ◽  
pp. 339-345 ◽  
Author(s):  
Kyung-Hwa Baek
Keyword(s):  
Population Dynamics ◽  
Crude Oil ◽  
Real Time ◽  
Contaminated Soil ◽  
Real Time Pcr ◽  
Bacterial Population

scholarly journals Sunflower seed husk combined with poultry droppings to degrade petroleum hydrocarbons in crude oil-contaminated soil

2020 ◽  
Vol 26 (5) ◽  
pp. 200361-0
Author(s):  
Sinduja Boopathy ◽  
Merline Sheela Appavoo ◽  
Ilamathi Radhakrishnan
Keyword(s):  
Moisture Content ◽  
Crude Oil ◽  
Contaminated Soil ◽  
Sunflower Seed ◽  
Bacterial Population ◽  
Dry Weight ◽  
Germination Percentage ◽  
Lady’S Finger ◽  
Seed Husk ◽  
Poultry Droppings

To remediate crude oil-contaminated soil, poultry droppings and sunflower seed husk were incorporated at various concentrations. Initially, the pH, moisture content, total organic carbon (TOC), total nitrogen content, and bacterial population of soil, poultry droppings, and sunflower seed husk were determined. The initial bacterial population was 35 × 10<sup>8</sup> colony forming units/g soil (dry weight basis). The total petroleum hydrocarbon (TPH) content in crude oil-contaminated soil was 7,048.4 mg/kg, in which 857.9 mg/kg accounted for eicosane. Naphthalene and acenaphthalene were the two polyhydroxy aromatic hydrocarbons present in soil at low concentrations. After the amendment, the pH, moisture content, microbial population, and TPH content of soil were determined on days 20, 40, and 60. The TPH concentration was considerably decreased in the treatment T4 wherein 250 g of soil was mixed with 125 g of poultry droppings and 125 g of sunflower seed husk. The indigenous bacterial population was also increased tremendously. The dehydrogenase enzyme activity was increased in the amended soil (T4: 0.74 ± 0.06 μg TPF/g/h). After the treatment of soil, the germination percentage and vigour index of maize, lady’s finger and tomato seeds were enhanced.

scholarly journals Microarray analysis and real-time PCR assay developed to find biomarkers for mercury-contaminated soil

2016 ◽  
Vol 5 (6) ◽  
pp. 1539-1547 ◽  
Author(s):  
Jing Hou ◽  
Xinhui Liu ◽  
Baoshan Cui ◽  
Junhong Bai ◽  
Xiangke Wang
Keyword(s):  
Adverse Effects ◽  
Human Health ◽  
Real Time ◽  
Microarray Analysis ◽  
Food Chain ◽  
Contaminated Soil ◽  
Real Time Pcr ◽  
Agricultural Soil ◽  
Pcr Assay ◽  
Soil P

The evaluation of mercury (Hg) toxicity in agricultural soil is of great concern because its bioavailability and bioaccumulation in organisms through the food chain can have adverse effects on human health.

Using Real-time PCR to assess changes in the crude oil degrading microbial community in contaminated seawater mesocosms

2014 ◽  
Vol 93 ◽  
pp. 241-248 ◽  
Author(s):  
Mehdi Hassanshahian ◽  
Michail M. Yakimov ◽  
Renata Denaro ◽  
Maria Genovese ◽  
Simone Cappello
Keyword(s):  
Microbial Community ◽  
Crude Oil ◽  
Real Time ◽  
Real Time Pcr
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