Optimization of Capsular Polysaccharide Production by Streptococcus pneumoniae Type 3

2009 ◽  
Vol 19 ◽  
Author(s):  
Sheng-De Jin
Keyword(s):  
Streptococcus Pneumoniae ◽  
Capsular Polysaccharide ◽  
Polysaccharide Production ◽  
Type 3

ChemInform Abstract: Two Synthetic Antigens Related to Streptococcus pneumoniae Type 3 Capsular Polysaccharide.

1986 ◽  
Vol 17 (6) ◽  
Author(s):  
A. YA. CHERNYAK ◽  
K. V. ANTONOV ◽  
N. K. KOCHETKOV ◽  
L. N. PADYUKOV ◽  
N. V. TSVETKOVA
Keyword(s):  
Streptococcus Pneumoniae ◽  
Capsular Polysaccharide ◽  
Type 3 ◽  
Synthetic Antigens

Synthesis of a hexasaccharide fragment of the capsular polysaccharide of Streptococcus pneumoniae type 3

2002 ◽  
Vol 80 (1) ◽  
pp. 76-81 ◽  
Author(s):  
Dirk J Lefeber ◽  
Eneko Aldaba Arévalo ◽  
Johannis P Kamerling ◽  
Johannes FG Vliegenthart
Keyword(s):  
Streptococcus Pneumoniae ◽  
Key Words ◽  
Selective Oxidation ◽  
Capsular Polysaccharide ◽  
Building Blocks ◽  
Oligosaccharide Synthesis ◽  
Tempo Oxidation ◽  
Carboxylic Groups ◽  
New Generation ◽  
Type 3

In the framework of the development of a new generation of neoglycoconjugate vaccines against Streptococcus pneumoniae, the synthesis is described of a spacer-containing hexasaccharide fragment related to the capsular polysaccharide of S. pneumoniae type 3. Hexasaccharide β-D-GlcpA-(1[Formula: see text]4)-β-D-Glcp-(1[Formula: see text]3)-β-D-GlcpA-(1[Formula: see text]4)-β-D- Glcp-(1[Formula: see text]3)-β-D-GlcpA-(1[Formula: see text]4)-β-D-Glcp-(1[Formula: see text]O-(CH2)3NH2) (1), comprised of three repeating units, was synthesized via a blockwise strategy employing suitably protected disaccharide building blocks. Carboxylic groups were introduced by selective oxidation with TEMPO (2,2,6,6-tetramethyl-1-piperidinyloxy) in the last reaction steps. Deprotection afforded target hexasaccharide 1.Key words: oligosaccharide synthesis, Streptococcus pneumoniae type 3, TEMPO oxidation.

scholarly journals Role of Streptococcus pneumoniae OM001 operon in capsular polysaccharide production, virulence and survival in human saliva

PLoS ONE ◽  
2018 ◽  
Vol 13 (1) ◽  
pp. e0190402 ◽  
Author(s):  
Zuleeza Ahmad ◽  
Richard M. Harvey ◽  
James C. Paton ◽  
Alistair J. Standish ◽  
Renato Morona
Keyword(s):  
Streptococcus Pneumoniae ◽  
Capsular Polysaccharide ◽  
Human Saliva ◽  
Polysaccharide Production

Synthesis of oligosaccharides structurally related to fragments of Streptococcus pneumoniae type 3 capsular polysaccharide

2017 ◽  
Vol 66 (1) ◽  
pp. 111-122 ◽  
Author(s):  
Yu. E. Tsvetkov ◽  
D. V. Yashunsky ◽  
E. V. Sukhova ◽  
E. A. Kurbatova ◽  
N. E. Nifantiev
Keyword(s):  
Streptococcus Pneumoniae ◽  
Capsular Polysaccharide ◽  
Type 3

Measurement of the humoral immune response against Streptococcus pneumoniae type 3 capsular polysaccharide and oligosaccharide containing antigens by ELISA and ELISPOT techniques

1988 ◽  
Vol 106 (1) ◽  
pp. 101-107 ◽  
Author(s):  
Guy J.W.J. Zigterman ◽  
AndréF.M. Verheul ◽  
Erna B.H.W. Ernste ◽  
Ronald F.M. Rombouts ◽  
Marinus J. De Reuver ◽  
...  
Keyword(s):  
Immune Response ◽  
Streptococcus Pneumoniae ◽  
Humoral Immune Response ◽  
Capsular Polysaccharide ◽  
Humoral Immune ◽  
Type 3

scholarly journals Monoclonal antibodies against protease-sensitive pneumococcal antigens can protect mice from fatal infection with Streptococcus pneumoniae.

1984 ◽  
Vol 160 (2) ◽  
pp. 386-397 ◽  
Author(s):  
L S McDaniel ◽  
G Scott ◽  
J F Kearney ◽  
D E Briles
Keyword(s):  
Cell Wall ◽  
Monoclonal Antibodies ◽  
Streptococcus Pneumoniae ◽  
Capsular Polysaccharide ◽  
Minimum Amount ◽  
Binding Studies ◽  
Fatal Infection ◽  
Rough Strain ◽  
Type 3

Monoclonal antibodies were raised against surface determinants of Streptococcus pneumoniae by hyperimmunizing X-linked immunodeficient (xid) CBA/N mice with the heat-killed rough strain R36A. 17 hybridomas produced antibody that bound intact R36A and did not cross-react with phosphocholine, an antigen common in the cell wall of all S. pneumoniae. The antibody produced by at least two of these hybridomas, Xi64 (IgM) and Xi126 (IgG2b), could protect mice from a lethal intravenous challenge of type 3 S. pneumoniae strains WU2 and A66 and of the type 2 strain D39. The minimum amount of antibody required to protect xid mice from 100 WU2 was 4.5 micrograms/mouse for Xi64 and 2.6 micrograms/mouse for Xi126,. Free phosphocholine, C-polysaccharide, and type 3 capsular polysaccharide all failed to inhibit the binding of Xi64 or Xi126 to R36A. These antibodies appeared to bind surface polypeptides, since treatment of R36A with either pepsin or trypsin, or of R36A lysate with trypsin, effectively eliminated the ability of Xi64 and Xi126 to bind antigens in these preparations. Binding studies indicated that these two antibodies recognized different epitopes that were expressed on several but not all serotypes of pneumococci.

scholarly journals Characterization of the Type 8 Capsular Gene Cluster of Streptococcus pneumoniae

1999 ◽  
Vol 181 (19) ◽  
pp. 6214-6219 ◽  
Author(s):  
Rosario Muñoz ◽  
Marta Mollerach ◽  
Rubens López ◽  
Ernesto García
Keyword(s):  
Streptococcus Pneumoniae ◽  
Nucleotide Sequence ◽  
Gene Cluster ◽  
Complete Nucleotide Sequence ◽  
Capsular Polysaccharide ◽  
Open Reading Frames ◽  
Dna Fragment ◽  
Type 3 ◽  
Reading Frames

ABSTRACT The complete nucleotide sequence of the capsular gene cluster (cap8) responsible for the biosynthesis of the capsular polysaccharide of Streptococcus pneumoniae type 8 has been determined. The cap8 gene cluster, located between the genes dexB and aliA, is composed of 12 open reading frames. A 14.7-kb DNA fragment embracing the cap8genes was sufficient to transform an unencapsulated type 3 S. pneumoniae strain to a strain with the type 8 capsule. A possible scenario for the evolution of pneumococcal types 2 and 8 is outlined.

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