Application of Scale-Up Criterion of Constant Oxygen Mass Transfer Coefficient (kLa) for Production of Itaconic Acid in a 50 L Pilot-Scale Fermentor by Fungal Cells of Aspergillus terreus

2013 ◽  
Vol 23 (10) ◽  
pp. 1445-1453 ◽  
Author(s):  
Woo-Shik Shin
Author(s):  
Haider Ali ◽  
Sofia Zhu ◽  
Jannike Solsvik

Abstract Scaling up stirred tanks is a significant challenge because of the research gaps between laboratory and industrial-scale setups. It is necessary to understand the effects of scale-up on the mass transfer in stirred tanks, and this requires meticulous experimental analysis. The present study investigates the effects of tank size and aspect ratio ( H L T ${H}_{L}}{T}$ ) on the volumetric mass transfer coefficients of shear-thinning fluids. The experiments were conducted in three stirred tanks of different sizes (laboratory and pilot scale) and geometries (standard and nonstandard). H L T ${H}_{L}}{T}$ was 1 for the standard tanks and 3.5 for the nonstandard stirred tanks. Three sizes of stirred tanks were used: 11 L with H L T ${H}_{L}}{T}$ of 1, 40 L with H L T ${H}_{L}}{T}$ of 3.5, and 47 L with H L T ${H}_{L}}{T}$ of 1. Impeller stirring speeds and gas flow rates were in the range of 800–900 rev min−1 and 8–10 L min−1, respectively. The volumetric mass transfer coefficient was estimated based on the dissolved oxygen concentration in the fluids, and the effects of rheology and operating conditions on the volumetric mass transfer coefficient were observed. The volumetric mass transfer coefficient decreased as tank size increased and increased with an increase in operating conditions, but these effects were also clearly influenced by fluid rheology. The impacts of scale-up and operating conditions on the volumetric mass transfer coefficient decreased as liquid viscosity increased.


Author(s):  
Williams Olughu ◽  
Duncan Galbraith ◽  
Cillian Paget ◽  
Steve Ruscoe ◽  
Josh Smith ◽  
...  

AbstractThe volumetric oxygen mass transfer coefficient ($$k_{l} a$$ k l a ) is an essential parameter in aerobic high-cell density fermentation where the availability of oxygen to growing microorganisms is a limiting factor. Bioprocess teams looking to scale-up/down between the Eppendorf BioBLU 0.3f single-use vessel and the BioFlo® 320 reusable vessel bioreactors may find it challenging using a matched $$k_{l} a$$ k l a . The maximum $$k_{l} a$$ k l a of the BioFlo® 320 reusable bioreactor was 109 h−1, which was approximately twice that of the BioBLU 0.3f single-use vessel. The results here show no overlap in $$k_{l} a$$ k l a values when both bioreactors were compared and thus conclude that scalability based on $$k_{l} a$$ k l a is not viable. The maximum $$k_{l} a$$ k l a of the Eppendorf BioBLU 0.3f single-use reported here was 47 h−1 compared to that of the manufacturer’s value of 2500 h−1, indicating a 53-fold difference. This discrepancy was attributed to the incompatible sulfite addition method used by the manufacturer for estimation.


2017 ◽  
Vol 40 (5) ◽  
pp. 878-888 ◽  
Author(s):  
Radim Petříček ◽  
Libor Labík ◽  
Tomáš Moucha ◽  
František Jonáš Rejl ◽  
Lukáš Valenz ◽  
...  

2014 ◽  
Vol 37 (3) ◽  
pp. 519-526 ◽  
Author(s):  
Ellen Cristina Souza ◽  
Dante Augusto Moraes ◽  
Thereza Christina Vessoni-Penna ◽  
Attilio Converti ◽  
Ricardo Pinheiro de Souza Oliveira

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