Genome-wide transcriptomic analysis of n-caproic acid production in Ruminococcaceae bacterium CPB6 with lactate supplementation

The proteolytic activity and volatile fatty acid production of 10 isolates of Clostridium botulinum type F from diverse geographical locations were determined. Two of the 10 strains were non-proteolytic, 3 were slightly proteolytic, and 5 were strongly proteolytic. The non-proteolytic cultures and the slightly proteolytic cultures produced acetic and butyric acid. The strongly proteolytic cultures produced mainly acetic, butyric, isobutyric, and isovaleric acid, and small to trace amounts of propionic, isocaproic, and caproic acid. The relative amounts of the various acids produced were markedly influenced by the growth medium. The addition of glucose to the growth medium caused an increase in the relative amount of butyric acid and a decrease in isobutyric and isovaleric acid.

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Genome-wide transcriptomic analysis of a flocculent strain of Zymomonas mobilis

Applied Microbiology and Biotechnology ◽

10.1007/s00253-012-3948-9 ◽

2012 ◽

Vol 93 (6) ◽

pp. 2513-2518 ◽

Cited By ~ 15

Author(s):

Young Jae Jeon ◽

Zhao Xun ◽

Ping Su ◽

Peter L. Rogers

Keyword(s):

Zymomonas Mobilis ◽

Transcriptomic Analysis ◽

Genome Wide

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Genetic factors underlying boron toxicity tolerance in rice: genome-wide association study and transcriptomic analysis

Journal of Experimental Botany ◽

10.1093/jxb/erw423 ◽

2016 ◽

pp. erw423 ◽

Cited By ~ 2

Author(s):

Joao Braga de Abreu Neto ◽

María Clara Hurtado-Perez ◽

Monika A. Wimmer ◽

Michael Frei

Keyword(s):

Association Study ◽

Genetic Factors ◽

Genome Wide Association Study ◽

Rice Genome ◽

Transcriptomic Analysis ◽

Genome Wide Association ◽

Boron Toxicity ◽

Genome Wide

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Development of a highly specific and productive process for n-caproic acid production: applying lessons from methanogenic microbiomes

Water Science & Technology ◽

10.2166/wst.2013.549 ◽

2013 ◽

Vol 69 (1) ◽

pp. 62-68 ◽

Cited By ~ 46

Author(s):

M. T. Agler ◽

C. M. Spirito ◽

J. G. Usack ◽

J. J. Werner ◽

L. T. Angenent

Keyword(s):

Carboxylic Acids ◽

Acid Production ◽

Waste Treatment ◽

Liquid Product ◽

Ph Control ◽

Caproic Acid ◽

Carbon Chain ◽

Chain Elongation ◽

Acid Extraction ◽

High Productivity

High productivity and specificity in anaerobic digesters arise because complex microbiomes organize into a metabolic cascade to maximize energy recovery and to utilize the advantage that the gaseous end product methane freely bubbles out of the system. These lessons were applied to ascertain whether a reactor microbiome could be shaped to produce a different end product. The liquid product n-caproic acid was chosen, which is a 6-carbon-chain carboxylic acid that is valuable and that has a relatively low maximum solubility concentration for product recovery. Acetoclastic methanogenesis was inhibited by pH control and a route was provided for n-caproic acid extraction by implementing selective, in-line recovery. Next, ethanol was supplemented to promote chain elongation, which is a pathway in which short-chain carboxylic acids are elongated sequentially into medium-chain carboxylic acids with two-carbon units derived from ethanol. The reactor microbiome developed accordingly with the terminal process catalyzed by chain-elongating bacteria. As a result, n-caproic acid production rates increased to levels comparable to anaerobic digestion systems for solid waste treatment.

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