An Investigation of the Solubilization of Primary Sewage Sludge using Lactic Acid Bacteria Cultured in a Glucose and Yeast Extract Medium

2012 ◽  
Vol 40 (4) ◽  
pp. 424-429 ◽  
Author(s):  
Sang Min Lee
Keyword(s):  
Lactic Acid ◽  
Sewage Sludge ◽  
Lactic Acid Bacteria ◽  
Yeast Extract ◽  
Yeast Extract Medium ◽  
Extract Medium

Isolation and identification of N2-fixing Pseudomonas associated with wetland rice

1983 ◽  
Vol 29 (8) ◽  
pp. 867-873 ◽  
Author(s):  
W. L. Barraquio ◽  
J. K. Ladha ◽  
I. Watanabe
Keyword(s):  
New Species ◽  
Yeast Extract ◽  
Heterotrophic Bacteria ◽  
Fluorescent Antibody ◽  
Wetland Rice ◽  
Isolation And Identification ◽  
Biochemical Characteristics ◽  
Yeast Extract Medium ◽  
Extract Medium ◽  
A New Species

Semisolid yeast extract medium amended with glucose and tryptic soy agar were used to isolate aerobically N2-fixing (C2H2-reducing) heterotrophic bacteria from the root of wetland rice. The isolates were identified as Pseudomonas by gel immunodiffusion and fluorescent antibody techniques in combination with their morphological, cultural, and biochemical characteristics. The N2-fixing H2-utilizing Pseudomonas described in this paper is a new species.

STRAIN IMPROVEMENT OF A FUNGUS PRODUCING CHITINASE BY A CHEMICAL MUTAGEN

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (11) ◽  
pp. 25-28
Author(s):  
K Narayanan ◽  
◽  
N.D. Chopade ◽  
V.M Subrahmanyam ◽  
J. Venkata Rao
Keyword(s):  
Protein Content ◽  
Ethidium Bromide ◽  
Yeast Extract ◽  
Wild Strain ◽  
Strain Improvement ◽  
Cost Effective ◽  
Specific Protein ◽  
Chemical Mutagen ◽  
Yeast Extract Medium ◽  
Extract Medium

Microbial chitinases are commercially exploited for their biocontrol properties and generation of useful products from chitinous waste. Availability of highly active chitinolytic enzymes is a major problem. The present study was carried out to improve chitinase production by Aspergillus terreus using a chemical mutagen, ethidium bromide. The organism was cultivated on lactose- yeast extract medium. The production medium consisting of chitin- yeast extract medium was seeded at 10% level. The wild strains were exposed to ethidium bromide in the concentration range 1.5- 6.0 µg/mL. Generally, all the mutated strains showed an improved chitinase yield compared to the control. Highest yield was observed with the strain exposed to 6 µg/mL of ethidium bromide. The yield was 25.03 % higher compared to the wild strain. The mutated strain was slimy in nature. Protein content of the mutated strain decreased by 11%. Ethidium bromide at a concentration of 1.5 µg/mL was considered optional, at which the strain was stable with increase of 21.80 % in enzyme activity and 4.41% increase in protein content. Increased enzyme yield with decreased non-specific protein could be useful in producing cost effective enzyme.

scholarly journals Effects of Epiphytic Enterobacteriaceae and Pseudomonads on the Growth of Listeria monocytogenes in Model Media

2001 ◽  
Vol 64 (5) ◽  
pp. 721-724 ◽  
Author(s):  
J. DEL CAMPO ◽  
F. CARLIN ◽  
C. NGUYEN-THE
Keyword(s):  
Amino Acids ◽  
Listeria Monocytogenes ◽  
Yeast Extract ◽  
Inhibitory Activity ◽  
Minimal Medium ◽  
Pseudomonas Chlororaphis ◽  
Enterobacter Agglomerans ◽  
Minimally Processed ◽  
Yeast Extract Medium ◽  
Extract Medium

Four Enterobacteriaceae (Enterobacter agglomerans and Rhanella aquatilis) and six pseudomonads (Pseudomonas fluorescens, Pseudomonas chlororaphis, Pseudomonas putida) isolated from minimally processed green endive were coinoculated at 10°C with Listeria monocytogenes in a minimal medium. Pseudomonads did not modify the growth of L. monocytogenes, whereas Enterobacteriaceae reduced its maximal population by 2 to 3 log CFU/ml. The same effect was observed in a diluted yeast extract medium supplemented with amino acids and glucose, in which L. monocytogenes grown alone reached 109 to 1010 CFU/ml. In the same diluted yeast extract medium, not supplemented with glucose and amino acids, the maximal population of L. monocytogenes in the presence of both Enterobacteriaceae and pseudomonads was only slightly reduced (less than 0.5 log CFU/ml). Culture filtrates of the Enterobacteriaceae had no inhibitory activity on L. monocytogenes. The effect of the Enterobacteriaceae on L. monocytogenes growth was presumably due to a competition for glucose and/or amino acids.

Media for Confirming Clostridium perfringens from Food and Feces

1978 ◽  
Vol 41 (8) ◽  
pp. 626-630 ◽  
Author(s):  
S. M. HARMON ◽  
D. A. KAUTTER
Keyword(s):  
Clostridium Perfringens ◽  
Yeast Extract ◽  
Nitrate Medium ◽  
Yeast Extract Medium ◽  
Selective Plating ◽  
Extract Medium

Several media recommended for confirming isolates of Clostridium perfringens from selective plating media were evaluated. Media for testing motility, reduction of nitrate to nitrite, fermentation of lactose, and liquefaction of gelatin were found to be the most useful. A modified motility-nitrate medium, developed during the study, and lactose-gelatin medium were the most satisfactory for doing these tests. Fermentation of salicin and raffinose in peptone-yeast extract medium was also useful for differentiating atypically reacting strains of C. perfringens from a variety of culturally similar clostridia.

Factors affecting the production of hydrogenase by Desulfovibrio desulfuricans

1980 ◽  
Vol 26 (10) ◽  
pp. 1209-1213 ◽  
Author(s):  
S. M. Martin ◽  
B. R. Glick ◽  
W. G. Martin
Keyword(s):  
Ammonium Sulfate ◽  
Yeast Extract ◽  
Desulfovibrio Desulfuricans ◽  
The Other ◽  
Hydrogenase Activity ◽  
Factors Affecting ◽  
Yeast Extract Medium ◽  
Extract Medium ◽  
Culture Development

An examination of conditions for the growth of Desulfovibrio desulfuricans, with the aim of optimizing hydrogenase production, is reported. An ammonium sulfate – lactate – yeast extract medium gave 5 to 10 times as much hydrogenase activity as a peptone – yeast extract medium. It made little if any difference whether the gas used for sparging was nitrogen, hydrogen, or a mixture thereof but increasing the rate of sparging and agitation did result in a slight decrease in activity. Control of pH during culture development was of little benefit to hydrogenase production. At least two hydrogenases were present in D. desulfuricans: one periplasmic, the other membrane bound.Desulfovibrio desulfuricans produced more hydrogenase than did either D. gigas and D. vulgaris.

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