Degradation Microorganisms Filtration of Petroleum Hydrocarbon in Tropic Ocean

2014 ◽  
Vol 1010-1012 ◽  
pp. 737-741
Author(s):  
Zeng Jian Su ◽  
Min Li ◽  
Yu Xiu Zhang
Keyword(s):  
Petroleum Hydrocarbon ◽  
Sole Carbon Source ◽  
Degradation Rate ◽  
Oil Pollution ◽  
Diesel Oil ◽  
Hainan Province ◽  
Oil Degradation ◽  
Test Results ◽  
Dominant Bacteria ◽  
Physiological And Biochemical

Six oil degradation strains were obtained from the tropic ocean of Hainan province using diesel oil as the sole carbon source in the research, which aim is to prevent and restore the ocean oil pollution at present and in future. The degradation rate of diesel oil by these strains was tested and three stains were filtrated as the dominant bacteria from Soh7, Soh11, Soh23, Soh26, Soh38 and Soh53 which were Soh7, Soh11 and Soh53. The 15d DR were 40.6%,31.3%,37.1% and 25d DR were 51.2%, 40.3%, 47.8% (TDR were 63.1%, 54.9%, 60.6%) separately under 24°C. Based on the morphological, physiological and biochemical test results, the Soh7 was identified asSporolactobacillus sp.and Soh11 and Soh53 wereAcidothermus sp..

Isolation of Petroleum Hydrocarbon Degrading Bacteria from the Liaohe Estuary at Cold Climate

2015 ◽  
Vol 1092-1093 ◽  
pp. 878-881
Author(s):  
Ping Guo ◽  
Jian Guo Lin ◽  
Bin Xia Cao ◽  
Na Ta
Keyword(s):  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Degradation Rate ◽  
Diesel Oil ◽  
Cold Climate ◽  
Hydrocarbon Degradation ◽  
Oil Degradation ◽  
Degrading Bacteria ◽  
Cold Tolerant ◽  
Diesel Oil Degradation

Two cold-tolerant petroleum hydrocarbon degrading bacteria strain named CHD1 and CHD2 were isolated from oil-contaminated soil at cold climate. The isolated strains were able use diesel oil as sole carbon. The petroleum hydrocarbon degradation rate was analyzed using UV-spectrometry-based methods. The results showed that the diesel oil degradation rate of CHD1 and CHD2 were 22% and 25%, respectively.

EM Biodegrading Characteristics of Petroleum Hydrocarbon in Artificial Seawater

2011 ◽  
Vol 356-360 ◽  
pp. 1145-1151
Author(s):  
Zhe Zhang ◽  
Shi Qiang Ding ◽  
Yu Suo Yang ◽  
Xiu Jin Zuo ◽  
Yin Xu
Keyword(s):  
Petroleum Hydrocarbon ◽  
High Efficiency ◽  
Oil Pollution ◽  
Optimal Growth ◽  
Diesel Oil ◽  
Artificial Seawater ◽  
Oil Degradation ◽  
Nacl Concentration ◽  
Diesel Oil Degradation ◽  
Seawater Medium

In this study, the feasible condition for diesel oil degradation of EM was investigated in artificial seawater medium. Under the optimal growth and degradation condition of pH 8.0, 30°C, inoculation amount of 3%, substrate concentration of 0.5%, and NaCl concentration of 20 g/L, the n-alkanes of diesel oil was degraded in high efficiency with total degrading rate of 72.3%, and the average n-alkanes (C10- C21) removal could reach 74.8% within 15 days. The results indicated that EM could be used in the bioremediation of marine oil pollution.

The Stress Response and Remediation of Plant on Oil-Contaminated Soil

2010 ◽  
Vol 113-116 ◽  
pp. 1195-1198 ◽  
Author(s):  
Min Qu ◽  
Yan Ming Zhang
Keyword(s):  
Contaminated Soil ◽  
Oil Pollution ◽  
Germination Rate ◽  
Soluble Sugars ◽  
Oil Degradation ◽  
Comprehensive Comparison ◽  
Morphological Differences ◽  
Soil Activity ◽  
Self Protection ◽  
Physiological And Biochemical

Oil pollution is seriously harmful to soil environment and human health. In order to verify the physiological and biochemical response of phytoremediation to oil-contaminated soil and test the relationship between the soil activity and the rate of oil degradation, in the present study, alfalfa, ryegrass, marigold and cosmos are used as the test plants. Through observing the morphological differences, such as germination rate, seedling height and root length, of four plant species under different concentrations of oil-contaminated soil, and measuring the physiological and biochemical indexes, which including MDA, soluble sugars, free proline, soluble protein and chlorophyll etc, and the change of oil degradation rate during the growth process of plant, results proved that plants could improve self-protection capabilities to resist the oil pollution by accumulating osmoregulation substances when they were subject to oil-contaminated stress. By analyzing the changes of polyphenol oxidase, it indicated that the plants can regulate enzyme activity in the oil-contaminated soil, and enhanced it. Comprehensive comparison of the above indexes, we draw a conclusion that alfalfa is an ideal species to repair the oil-contaminated soiland Marigold is the second candidate. In general, our work will lay the theoretic basis for phytoremediation technology research.

Isolation and Characterization of Petroleum Hydrocarbon Degrading Bacteria from the Bohai Sea, China

2014 ◽  
Vol 955-959 ◽  
pp. 728-731
Author(s):  
Ping Guo ◽  
Jian Guo Lin ◽  
Bin Xia Cao ◽  
Na Ta
Keyword(s):  
Petroleum Hydrocarbon ◽  
Bohai Sea ◽  
Diesel Oil ◽  
Contaminated Site ◽  
Oil Degradation ◽  
Isolation And Characterization ◽  
Degrading Bacteria ◽  
Luxuriant Growth ◽  
Diesel Oil Degradation

Fourteen petroleum hydrocarbon degrading bacteria strains were isolated from oil-contaminated site. Isolated strains were able use diesel oil as sole carbon and energy source. Bacterial strain HD1 was selected due to the luxuriant growth on oil agar. The oil degradation rate of strain HD1 was analyzed using UV-spectrometry-based methods. The result showed that the rate of diesel oil degradation of 75% was observed after 14days of cultivation.

Study on the Isolation, Identification and Degradation Characterizaion of a DDT-Degrading Bacteria

2012 ◽  
Vol 518-523 ◽  
pp. 2030-2033 ◽  
Author(s):  
Jia Niu ◽  
Ji Hua Wang ◽  
Di Cui ◽  
Xiang Liu ◽  
Hui Guang
Keyword(s):  
Sole Carbon Source ◽  
Degradation Rate ◽  
Enrichment Culture ◽  
Contaminated Site ◽  
Positive Bacterium ◽  
Degrading Bacteria ◽  
Pseudomonas Species ◽  
Biochemical Identification ◽  
Bacterium Strain ◽  
Physiological And Biochemical

A Gram positive bacterium strain 12-3 for degrading DDT effectively was isolated from the DDT contaminated site of the shipyard in Guangzhou by enrichment culture, which could utilize DDT as the sole carbon source for growth. This strain was identified as Pseudomonas species. Based on the phenotype, physiological and biochemical identification, and fatty acids identification. Testing theirs DDT degradation rate with HPLC, the results showed that in a shaky flask containing 20 mg/L DDT, this strain could degrade DDT with degradation efficiency of 51.6% in 8 days at 30°C, pH 8.0.

scholarly journals Isolation and characterization of a high-efficiency marine diesel oil-degrading bacterium

2021 ◽  
Author(s):  
Jin Gao ◽  
Jie Ming ◽  
Meng Xu ◽  
Xinge Fu ◽  
Liang-Feng Duan ◽  
...  
Keyword(s):  
Degradation Rate ◽  
High Efficiency ◽  
Jiaozhou Bay ◽  
Diesel Oil ◽  
Oil Degradation ◽  
Single Factor ◽  
Degrading Bacterium ◽  
Isolation And Characterization ◽  
Oil Concentration ◽  
Diesel Oil Degradation

AbstractIn the recent 50 years, marine oil spills had resulted in severe environmental pollution problems worldwide. In this study, 12 petroleum-degrading strains named MJ1 to MJ12, which can use diesel oil as the sole carbon source for growth, were isolated from the seawater in Jiaozhou Bay, China. Strain MJ4 has the highest diesel-degrading rate which is up to 26.54% in 5 days with the diesel oil concentration of 10 g/L. According to the BLAST research, 16SrRNA sequence of MJ4 showed 99% similarity to Bacillus megaterium strain. Single-factor experiments and response surface methodology were carried out to optimize the environmental factors and their reciprocal action for affecting the diesel oil degradation process of Bacillus sp. MJ4. Results of single-factor experiments revealed that the highest degradation rate was obtained with temperature of 28 °C, pH of 8.8, diesel oil concentration of 25 g/L, P/N ratio of 0.56, nitrogen and phosphorus dosage quantity of 0.35 g/L and 0.18 g/L, respectively. A nonlinear regression equation of diesel oil degradation rate and pH, temperature, P/N ratio was obtained. The model predicted the maximum degradation rate of 72.21% with temperature of 28 °C, pH of 8.88, P/N ratio of 0.31, respectively.

scholarly journals Characterization of microbial response to petroleum hydrocarbon contamination in a lacustrine ecosystem

2021 ◽  
Author(s):  
Emilio D’Ugo ◽  
Milena Bruno ◽  
Arghya Mukherjee ◽  
Dhrubajyoti Chattopadhyay ◽  
Roberto Giuseppetti ◽  
...  
Keyword(s):  
Oil Spill ◽  
Petroleum Hydrocarbon ◽  
Oil Spills ◽  
Oil Pollution ◽  
Primary Source ◽  
Hydrocarbon Contamination ◽  
Petroleum Hydrocarbon Contamination ◽  
Microbial Response ◽  
Pollution Event ◽  
Hydrocarbonoclastic Potential

AbstractMicrobiomes of freshwater basins intended for human use remain poorly studied, with very little known about the microbial response to in situ oil spills. Lake Pertusillo is an artificial freshwater reservoir in Basilicata, Italy, and serves as the primary source of drinking water for more than one and a half million people in the region. Notably, it is located in close proximity to one of the largest oil extraction plants in Europe. The lake suffered a major oil spill in 2017, where approximately 400 tons of crude oil spilled into the lake; importantly, the pollution event provided a rare opportunity to study how the lacustrine microbiome responds to petroleum hydrocarbon contamination. Water samples were collected from Lake Pertusillo 10 months prior to and 3 months after the accident. The presence of hydrocarbons was verified and the taxonomic and functional aspects of the lake microbiome were assessed. The analysis revealed specialized successional patterns of lake microbial communities that were potentially capable of degrading complex, recalcitrant hydrocarbons, including aromatic, chloroaromatic, nitroaromatic, and sulfur containing aromatic hydrocarbons. Our findings indicated that changes in the freshwater microbial community were associated with the oil pollution event, where microbial patterns identified in the lacustrine microbiome 3 months after the oil spill were representative of its hydrocarbonoclastic potential and may serve as effective proxies for lacustrine oil pollution.

Oil pollution studies of the Solbergstrand mesocosms

1987 ◽  
Vol 316 (1181) ◽  
pp. 641-654 ◽  
Keyword(s):  
Dominant Species ◽  
Oil Pollution ◽  
Benthic Communities ◽  
Ascophyllum Nodosum ◽  
Diesel Oil ◽  
Benthic Macrofauna ◽  
Sediment Chemistry ◽  
Littorina Littorea ◽  
Species Structure ◽  
Laminaria Digitata

Two medium-scale ecosystems (mesocosms) were built on the Oslofjord: one a hard-bottom intertidal system and the other a subtidal soft-sediment system. The hard-bottom mesocosm consists of four basins, two controls and two which were dosed with diesel-oil (129 μg 1 -1 a high oil (HO) dose and 29 μg 1 -1 a low oil (LO) dose). Both oil doses caused high mortality of Mytilus edulis and growth was reduced in the macroalgae Ascophyllum nodosum and Laminaria digitata . Recruitment of Littorina littorea was also affected by oil so that populations declined over time. Subtidal benthic communities have been established in the mesocosm and show variations in sediment chemistry within the range found in the field. Although recruitment of benthic macrofauna is reduced, dominant species and species structure remain closely similar to that in the field over six months. Bioturbation effects studied in the mesocosm have shown the important influence of large, rare species in structuring benthic communities, a finding which would not be possible in nature by diving or by the use ofsubmersibles. Preliminary results from a community taken from 200 m depth and established in the mesocosm suggest that it is now possible to do detailed manipulation experiments on communities simulating the whole continental shelf.

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