Biologisation of production and technological processes in nursery farming

We conducted a retrospective analysis of national scientific and technological advances by technological design to manifest the demand for organisation processes remodelling towards the methods and approaches of the sixth design, especially in biotechnology. The article defines terms such as nursery, biologisation, promising technology and resource conservation. We analyse the fruit crop seedling production and structure of nursery-specific processes. We determine the main agrocenotic components most susceptible to chemical and technogenic impacts. Studies of soil fertility and biota prioritised the challenge of declined soil activity and biogenicity. We establish that an increased chemical pressure on fruit nursery agrocenoses leads to disturbances in benign microflora, microbiotic, acaro- and entomosystems, alters plant infection pathways and immune status. We report destructive manifestations of microbiotic, entomo- and acarosystems in agrocenoses via the emergence of new pathogenic fungal species, root rotting agents, vascular system necroses (tracheomycoses), resistant typically dominant pathogen strains, higher pathogenicity, the expansion of species list and ranges of bacterial communities, phytoplasmas, viruses and viroids, a more aggressive invasion of new pests, including stem pathogens, emerging hazardous adaptations in economically impactive phytophages. Furthermore, we consider the scientific and practical issues in fruit crop reproduction: sweeping off forms (genotypes) from selection, changes in infection pathways in candidate parental plants, reduced “plant — external environment” adaptation, impaired plant immunity under climatic and anthropogenic stress, selection of candidates with a higher production value under environmental stress burden, reduction of best-quality planting stock, seedling root system retardation, massive crown invasion with fungal and bacterial agents, inadequacy of trait databasing for promising varieties and genotyping techniques. The priority role of agrocenotic biologisation in sustainable fruit nursery is substantiated through adopting modern approaches, especially in biotechnology, based on molecular biology, biochemistry and genetic engineering.

Effects of Onion Residue, Bovine Manure Compost and Compost Tea on Soils and on the Agroecological Production of Onions

Organic solid wastes are rarely considered when planning for rural production in Argentina. Onion production in the low valley of Río Colorado (Buenos Aires) generates between 12,000 and 20,000 Mg year−1 of vegetal wastes (i.e., leaves, stems, skins, roots) from harvesting, cleaning and classification of bulbs, causing many problems with their management. The aim of this work is to study the effect of different doses of onion residue-bovine manure compost and onion residue-bovine manure compost tea on the soil physicochemical properties, microbial activity and agroecological onion production in sandy soil. Results showed that the highest dose of compost caused the highest effects on soil pH, electrical conductivity and nutrient content. Soil enzymatic activities were already high in the soil before the compost was applied, which may have contributed to the small effect caused by any dose on soil activity. A significant positive effect on bulb weight and organic onion yield were found as a result of the amendment and growing season. In conclusion, agroecological production of onion with the addition of a 300 kg N ha−1 compost and compost tea guarantee yields comparable to those of conventional fertilization, as occurred during the two growing seasons of this study.

Organic Constituents and Biota in the Urban Atmospheric Solid Aerosol: Potential Effects on Urban Soils

The main components of solid atmospheric aerosol are soil and rock particles raised from the earth’s surface by wind erosion, and primary biological aerosol particles. In the composition of atmospheric aerosol, many pollutants, both mineral and organic, appear in areas with intensive human activity. Summer dust (solid atmospheric fallouts) that fell out of atmosphere was collected at two sites in Moscow (the territory of the Leo Tolstoy Museum-Estate in Khamovniki and the Botanical Garden of the Biological Faculty of Moscow State University). Morphological and microbiological studies were carried out in order to characterize the composition of the organic part of urban solid atmospheric fallouts and its possible impact on soils and the urban ecosystem as a whole. It has been found that the composition of the organic part of the samples was identical and included: the representatives of aeroplankton and other particles of biological origin, and also fragments of oil films, plastic fibers, carbon particles, etc., which indicated the hydrocarbon and microplastic pollution brought from the atmosphere. The composition of the studied groups of microorganisms in atmospheric fallouts and in urban soils was similar and indicated close ecological links between urban dust aerosol and soils. The biomass of the studied groups of microorganisms of atmospheric solids was dominated by fungi, many of which are potentially pathogenic and allergenic organisms. Apparently, atmospheric solid aerosols are carriers of microbiological pollution associated with animal feces in the city. The presence of such particles in the air indicates insufficient soil activity as a “bacterial filter”.

Effects of Triazole Fungicides on Soil Microbiota and on the Activities of Enzymes Found in Soil: A Review

Triazole fungicides can manifest toxicity to a wide range of non-target organisms. Within this study we present a systematic review of the effects produced on the soil microbiota and activity of soil enzymes by the following triazole fungicides: cyproconazole, difenoconazole, epoxiconazole, flutriafol, hexaconazole, metconazole, myclobutanil, paclobutrazole, propiconazole, tebuconazole, tetraconazole, triadimenol, triadimefon, and triticonazole. Known effects of the triazole fungicides on the soil activity are dose dependent. High doses of triazole fungicides strongly affects the structure of the microbial communities in soil and usually decrease the soil microbial population and the activities of enzymes found in soil.

Study the influences of the radionuclide depth distributions on the FEPE for the measurements of the soil activity using in situ HPGe gamma spectrometry

In this work, the influences of the soil densities and the radionuclide depth distributions(RDD) on the Full Energy Peak Efficiency (FEPE) calculation of the in-situ gamma rayspectrometer using the In Situ Object Counting Systems (ISOCS) software were studied. The data of the RDDs at the sites were investigated by using laboratory HPGe gamma spectrometer. Six different RDDs of 40K, 226Ra and 232Th were found at four studied sites with radionuclide deposition moving from surface to deeper positions. The results show that FEPE values vary strongly for the different RDDs, especially for the low gamma ray energies. Use of the uniform model for calculating FEPEs can result in noticeable errors from 29% to 101% for the realistic RDD of the exponential form (surfaceradionuclide deposition), negative variations from 14% to 30% for the realistic RDD of having a radionuclide deposition at the 30 cm depth, and negligible variations of less than 5 % for the realistic RDD of quasi uniform form in the range of gamma ray energies of interest.

Effect of carbon-enriched digestate on the microbial soil activity

Objectives As a liquid organic fertilizer used in agriculture, digestate is rich in many nutrients (i.e. nitrogen, phosphorus, sulfur, calcium, potassium); their utilization may be however less efficient in soils poor in organic carbon (due to low carbon:nitrogen ratio). In order to solve the disadvantages, digestate enrichment with carbon-rich amendments biochar or humic acids (Humac) was tested. Methods Soil variants amended with enriched digestate: digestate + biochar, digestate + Humac, and digestate + combined biochar and humic acids—were compared to control with untreated digestate in their effect on total soil carbon and nitrogen, microbial biomass carbon, soil respiration and soil enzymatic activities in a pot experiment. Yield of the test crop lettuce was also determined for all variants. Results Soil respiration was the most significantly increased property, positively affected by digestate + Humac. Both digestate + biochar and digestate + Humac significantly increased microbial biomass carbon. Significant negative effect of digestate + biochar (compared to the control digestate) on particular enzyme activities was alleviated by the addition of humic acids. No significant differences among the tested variants were found in the above-ground and root plant biomass. Conclusions The tested organic supplements improved the digestate effect on some determined soil properties. We deduced from the results (carbon:nitrogen ratio, microbial biomass and activity) that the assimilation of nutrients by plants increased; however, the most desired positive effect on the yield of crop biomass was not demonstrated. We assume that the digestate enrichment with organic amendments may be more beneficial in a long time-scaled trial.

The Effects of Various Doses and Types of Effective Microorganism Applications on Microbial and Enzyme Activity of Medium and the Photosynthetic Activity of Scarlet Sage

The aim of this study was to examine the influence of various types and levels of effective microorganism (EM) applications on scarlet sage. For this purpose, EMs were applied at the following three concentrations: 1:10, 1:50, and 1:100. Moreover, two types of treatments (spraying and watering) and a combination of the two were also examined. Photosynthetic intensity was analyzed, including the net photosynthesis rate (PN), stomatal conductance (gS), and intercellular CO2 concentration (Ci). Additionally, chlorophyll a, b, and a + b, and the chlorophyll b/a ratio were analyzed. The microbial content in the medium and soil enzyme activity were also evaluated to examine the effect of EMs on soil biological properties. The investigations revealed a high positive effect of EMs on the photosynthetic activity of most EM combinations compared with the control. The greatest positive effect was noted for the highest EM concentration application for both types of treatments. There was no such influence on soil activity. An increase was noted only in the number of fungi and dehydrogenase activity, while the rest of the soil biological status parameters revealed significant variability, and mostly small or no effects were recorded.