Growth Conditions of a Thermophilic Geobacillus sp. as an Enhanced Oil Recovery Material

The growth conditions of a Geobacillus sp. were investigated by single-factor experiments. The strain was strictly aerobic bacterium, and could grow on hydrocarbons as the sole carbon source. The optimum carbon and nitrogen sources were 3.0% sucrose and 0.20% KNO3, respectively. The range of temperature, salinity and pH for the bacterial growth was 35-70 °C, 0-10% NaCl and 5.5-9.5, and good growth was obtained at 35-65 °C, 0.5-8% NaCl and 6.0-9.0, respectively. Particularly, the optimum temperature for the bacterial growth was between 50 °C and 60 °C. The strain had wide adaptability to the extreme conditions, and may be potentially applied to microbial enhanced oil recovery and oil-waste bioremediation technology.

Antagonistic and synergistic effects of fungal and bacterial growth in soil after adding different carbon and nitrogen sources

Soil Biology and Biochemistry ◽

10.1016/j.soilbio.2008.05.011 ◽

2008 ◽

Vol 40 (9) ◽

pp. 2334-2343 ◽

Cited By ~ 117

Author(s):

Sandra Meidute ◽

Fredrik Demoling ◽

Erland Bååth

Keyword(s):

Bacterial Growth ◽

Synergistic Effects ◽

Nitrogen Sources ◽

Role of Carbon and Nitrogen Sources in Bacterial Growth and Sporulation

Applied Microbiology ◽

10.1128/am.22.4.740-740c.1971 ◽

1971 ◽

Vol 22 (4) ◽

pp. 740-740

Keyword(s):

Bacterial Growth ◽

Nitrogen Sources ◽

The preliminary optimization of culture components for improving antifungal production by a wild-type soil bacteria Bacillus subtilis

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11ispl4.4229 ◽

2020 ◽

Vol 11 (SPL4) ◽

pp. 955-961

Author(s):

Ramya Ramachandran ◽

Kanika Bhatnagar ◽

Ramlal Thakur ◽

Utpal Roy

Keyword(s):

Nitrogen Sources ◽

Growth Conditions ◽

Indicator Strain ◽

Coefficient Of Determination ◽

Bacillus Species ◽

Antifungal Compound ◽

Malt Extract ◽

Antifungal Compounds ◽

Antifungal compounds are produced by Bacillus species under various growth conditions. While optimizing the antifungal production by using the one-factor-at-a-time (OFAT) and response surface methodology (RSM) approaches mannose and malt extract were identified as the most suitable carbon and nitrogen sources respectively. The RSM was applied to determine the optimum conditions of the three parameters such as pH, carbon and nitrogen sources for improved production. Optimum concentrations of carbon and nitrogen sources were 0.3% and 0.6% respectively with optimum media pH of 7.0 which showed optimum value of 40 AU/ml of antifungal compound against the Candida albicans SC5314 used as an indicator strain. In the present study, the F-value was determined as 0.0034 to imply that the model is significant. The goodness of the fit of the model was tested using coefficient of determination, R2 value, that tantamounts to 0.8562. The identification of antifungal compounds with their molecular masses was accomplished by MALDI-TOF mass spectrometry after n- butanol extraction. The present study thus has provided a platform to upgrade the yield of antifungal compounds which have got immense clinical potential to tread Candidosis.

Role of Carbon and Nitrogen Sources in Bacterial Growth and Sporulation

Applied Microbiology ◽

10.1128/am.22.1.131-132.1971 ◽

1971 ◽

Vol 22 (1) ◽

pp. 131-132

Author(s):

Raizada M. M. Singh

Keyword(s):

Bacterial Growth ◽

Nitrogen Sources ◽

Nutritional requirements for growth of Cryptoporus volvatus

Canadian Journal of Botany ◽

10.1139/b89-326 ◽

1989 ◽

Vol 67 (8) ◽

pp. 2532-2534

Author(s):

Thomas M. Pettey

Keyword(s):

Carbon Source ◽

Glutamic Acid ◽

Nitrogen Source ◽

Agar Medium ◽

Nitrogen Sources ◽

Nutritional Requirements ◽

Good Growth ◽

Carbon And Nitrogen ◽

Partial Deficiency

Carbon and nitrogen sources were examined in a defined agar medium to determine the nutritional requirements of Cryptoporus volvatus, a Hymenomycete. Good growth was obtained with D-glucose, D-fructose, D-mannose, D-xylose, or dextrin as the carbon source. Good growth was obtained with ammonium sulfate, casein, peptone, glutamic acid, glycine, lysine, serine, or tyrosine as the nitrogen source. In a defined agar medium, C. volvatus exhibited a deficiency for thiamine, and a partial deficiency for biotin, inositol, and pyridoxine.

Microbial Cellulose Production from Bacteria Isolated from Rotten Fruit

International Journal of Polymer Science ◽

10.1155/2015/280784 ◽

2015 ◽

Vol 2015 ◽

pp. 1-8 ◽

Cited By ~ 23

Author(s):

B. E. Rangaswamy ◽

K. P. Vanitha ◽

Basavaraj S. Hungund

Keyword(s):

Biochemical Analysis ◽

Nitrogen Sources ◽

Growth Conditions ◽

Inoculum Density ◽

Bacterial Isolates ◽

Cellulose Production ◽

Microbial Cellulose ◽

Microbial cellulose, an exopolysaccharide produced by bacteria, has unique structural and mechanical properties and is highly pure compared to plant cellulose. Present study represents isolation, identification, and screening of cellulose producing bacteria and further process optimization. Isolation of thirty cellulose producers was carried out from natural sources like rotten fruits and rotten vegetables. The bacterial isolates obtained from rotten pomegranate, rotten sweet potato, and rotten potato were identified asGluconacetobactersp. RV28,Enterobactersp. RV11, andPseudomonassp. RV14 through morphological and biochemical analysis. Optimization studies were conducted for process parameters like inoculum density, temperature, pH, agitation, and carbon and nitrogen sources usingGluconacetobactersp. RV28. The strain produced 4.7 g/L of cellulose at optimum growth conditions of temperature (30°C), pH (6.0), sucrose (2%), peptone (0.5%), and inoculum density (5%). Characterization of microbial cellulose was done by scanning electron microscopy (SEM).