Effect of Four Materials Including Glycine, Arginine, Proline and Sodium Glutamate on Survival of Bifidobacterium bifidum during Freeze-Drying

2013 ◽  
Vol 676 ◽  
pp. 93-96
Author(s):  
Qi Ma ◽  
Tao Qin ◽  
He Chen ◽  
Guo Wei Shu
Keyword(s):  
Survival Rate ◽  
Freeze Drying ◽  
Bifidobacterium Bifidum ◽  
Sodium Glutamate ◽  
Viable Cells ◽  
Before And After

Effect of four materials including glycine, arginine, proline and sodium glutamate on survival of Bifidobacterium bifidum during freeze-drying was studied by measuring the viable counts of before and after freeze-drying. The addition of glycine, arginine, proline and sodium glutamate (%, w/v) were all 1.0%, 2.0%, 3.0%，4.0% and 5.0%. Results were as follows: addition of glycine, arginine, proline and sodium glutamate into B. bifidum paste could significantly improve the survival rate of B. bifidum and the number of viable cells of lyophilized powder. The optimal concentrations of glycine, arginine, proline and sodium glutamate were 4.0%, 4.0%, 3.0% and 2.0%, the survival of B. bifidum was 87.49%, 84.28%, 67.50% and 69.01%, respectively.

Effect of NaH2PO4, Na2HPO4, MgSO4, and Ascorbic Acid on Survival of Lactobacillus acidophilus during Freeze-Drying

2012 ◽  
Vol 568 ◽  
pp. 279-282
Author(s):  
He Chen ◽  
Zhen Xing Ma ◽  
Guo Wei Shu ◽  
Tao Qin
Keyword(s):  
Ascorbic Acid ◽  
Survival Rate ◽  
Lactobacillus Acidophilus ◽  
Freeze Drying ◽  
Protective Agent ◽  
Survival Ratio ◽  
Protective Agents ◽  
Viable Cells ◽  
Before And After

Effect of NaH2PO4, Na2HPO4, MgSO4, and Ascorbic acid on survival of Lactobacillus acidophilus was investigated before and after freeze-drying. Editing different concentrations of protective agents respectively during freeze-drying. After completion of the pilot, the survival ratio of Lactobacillus acidophilus and the number of viable cells were being measured. Results were as follows: the highest survival rate and the largest number of viable cells was Na2HPO4as the protective agent.

Effect of MgSO4, NaHCO3, KH2PO4, K2HPO4 and Ascorbic Acid on Survival of Bifidobacterium bifidum during Freeze-Drying

2013 ◽  
Vol 675 ◽  
pp. 346-349
Author(s):  
Tao Qin ◽  
Qi Ma ◽  
He Chen ◽  
Guo Wei Shu
Keyword(s):  
Ascorbic Acid ◽  
Survival Rate ◽  
Freeze Drying ◽  
Bifidobacterium Bifidum ◽  
Viable Cells

Effect of MgSO4, NaHCO3, KH2PO4, K2HPO4 and ascorbic acid on the survival of Bifidobacterium bifidum and the number of viable cells of lyophilized powder was studied. Results were as follows: addition of MgSO4, NaHCO3, KH2PO4, K2HPO4 and ascorbic acid into B. bifidum paste could significantly improve the survival rate of B. bifidum and the number of viable cells of lyophilized powder. The optimal concentrations of MgSO4, NaHCO3, KH2PO4 , K2HPO4 and ascorbic acid were 0.20%, 0.60%, 1.80%, 1.40% and 2.20, the survival of B. bifidum was 78.31%, 77.92%, 77.80% ,79.82% and 71.62, respectively.

scholarly journals Effects of Carbohydrates, Prebiotics and Salts on Survival of Saccharomyces boulardii During Freeze-Drying

2018 ◽  
Vol 22 (2) ◽  
pp. 59-66
Author(s):  
Guowei Shu ◽  
Xin Yang ◽  
Zhangteng Lei ◽  
Dan Huang ◽  
Yaling ZHAI
Keyword(s):  
Survival Rate ◽  
Phosphate Buffer ◽  
Freeze Drying ◽  
Saccharomyces Boulardii ◽  
Sodium Ascorbate ◽  
Sodium Glutamate ◽  
Viable Cells ◽  
Freeze Dried ◽  
Probiotic Yeast ◽  
Addition Amount

Abstract Saccharomyces boulardii, as a probiotic yeast, had been commonly used in food, medicine and feed to treat diarrhea in humans or livestock. However, there are few researches focusing on the preparation of its freeze-drying S.boulardii powder. In this study, the effect of carbohydrates (glucose, sucrose, maltose, fructose, lactose, mannose and trehalose), prebiotics (isomalto-oligosaccharide, xylo-oligosaccharide, raffinose, stachyose, inulin, galacto-oligosaccharide and fructo-oligosaccharide) and salts (NaHCO3, MgSO4, sodium glutamate, sodium ascorbate, and phosphate buffer) on the freeze-dried survival of S. boulardii were investigated to screen the cryoprotectant by using single factor experiments. As the result, trehalose and XOS had better protective effect, the survival rate was 23.72% and 20.70% respectively, the number of viable cells reached 0.91×1010 CFU/g and 0.85×1010 CFU/g respectively; the addition amount of NaHCO3 was 0.3%, the freeze-dried survival rate reached the maximum value of 12.92%. The phosphate buffer additive amount and the bacterial sludge weight were 0.8:1, the freeze-dried survival rate reached a maximum of 14.14%, the freeze-dried survival rate of sodium glutamate, sodium ascorbate and MgSO4 groups was increasing, reaching a maximum of 20.26%, 16.47% and 6.29% when the addition amount was 2%, 10%, 0.5%.

scholarly journals Effect of Sodium Tripolyphosphate, Glycerol, Vitamin B2, Mannitol and Solid Malt Extract on Survival of Bifidobacterium bifidum During Freeze-Drying

2019 ◽  
Vol 23 (1) ◽  
pp. 71-74
Author(s):  
Guowei Shu ◽  
Chun Yin ◽  
Haipeng Qin ◽  
Haiyan Kou ◽  
Shuai He ◽  
...  
Keyword(s):  
Survival Rate ◽  
Freeze Drying ◽  
Sodium Tripolyphosphate ◽  
Bifidobacterium Bifidum ◽  
Vitamin B ◽  
Malt Extract ◽  
Vitamin B2 ◽  
Single Factor ◽  
Viable Cells ◽  
Freeze Dried

Abstract The effect of sodium tripolyphosphate, glycerol, vitamin B2, mannitol and solid malt extract on the freeze-dried powder survival rate and the viable cells of the B. bifidum BB01 were studied by single-factor test in this study. The optimal concentration of protectant for B. bifidum during freeze-drying were 1.5% (W/V) sodium tripolyphosphate, 12% (W/V) glycerol, 6% (W/V) vitamin B2, 6% (W/V) mannitol and 6% (W/V) solid malt extract, and the survival rate of bacteria was 18.63%, 22.98%, 24.13%, 24.19% and 39.77%, respectively.

Effect of K2HPO4, KH2PO4, NaHCO3, and Sodium Ascorbate on Survival of Lactobacillus acidophilus during Freeze-Drying

2012 ◽  
Vol 531 ◽  
pp. 284-287 ◽  
Author(s):  
Zhen Xing Ma ◽  
He Chen ◽  
Guo Wei Shu ◽  
Tao Qin
Keyword(s):  
Survival Rate ◽  
Lactobacillus Acidophilus ◽  
Freeze Drying ◽  
Sodium Ascorbate ◽  
Protective Agent ◽  
Survival Ratio ◽  
Protective Agents ◽  
Viable Cells ◽  
Key Factor

In the present study, the experiments were investigated with the effecting of NaHCO3, KH2PO4, K2HPO4 and Sodium ascorbate on survival of Lactobacillus acidophilus during freeze-drying. Designing different concentrations of protective agents, respectively. The survival ratio of Lactobacillus acidophilus and the number of viable cells will be measured after completing drying. In the process, there were major differences between the different concentrations of protective agents in survival during freeze-drying. As the result, the protective agent was playing a key factor affecting the survival of Lactobacillus acidophilus. At present in the study of the experiments, the highest survival rate was NaHCO3 as the protective agent, and when the protective agent as K2HPO4 the number of viable cells was largest.

Effect of Sucrose, Lactose, Glucose, Maltose on Survival of Lactobacillus acidophilus during Freeze-Drying

2011 ◽  
Vol 382 ◽  
pp. 396-399 ◽  
Author(s):  
He Chen ◽  
Zhen Xing Ma ◽  
Guo Wei Shu ◽  
Tao Qin ◽  
Ning Gang Cai
Keyword(s):  
Survival Rate ◽  
Lactobacillus Acidophilus ◽  
Freeze Drying ◽  
Critical Factor ◽  
Soluble Starch ◽  
Protective Agent ◽  
Survival Ratio ◽  
Protective Agents ◽  
Viable Cells

In the present study, the experiments were investigated with effecting of sucrose, lactose, glucose, maltose on survival of Lactobacillus acidophilus during freeze-drying. In order to reach sufficient numbers viable cells, so different concentrations of protective agents were designed in the experiment, which as 3%, 6%, 9%, 12% and 15%, respectively. Measuring the survival ratio of Lactobacillus acidophilus and number of viable cells after completed drying. There were significant differences in survival during freeze-drying. Results showed that the protective agent was a critical factor affecting the survival of Lactobacillus acidophilus. In the experiments, the highest survival rate was soluble starch as the protective agent, and when the protective agent as galactose the number of viable cells was largest.

Effect of Four Materials Including Trehalose, Soluble Starch, Raffinose and Galactose on Survival of Lactobacillus acidophilus during Freeze-Drying

2013 ◽  
Vol 700 ◽  
pp. 259-262
Author(s):  
Tao Qin ◽  
Qi Ma ◽  
He Chen ◽  
Guo Wei Shu
Keyword(s):  
Lactobacillus Acidophilus ◽  
Freeze Drying ◽  
Soluble Starch ◽  
Viable Cells ◽  
Before And After

Effect of four materials including trehalose, soluble starch, raffinose and galactose on survival of Lactobacillus acidophilus was studied by counting viable cells before and after freeze-drying. The concentrations of five carbohydrates were all 3%, 6%, 9%, 12% and 15%. Results showed as follows: the optimal concentrations of trehalose, soluble starch, raffinose and galactose were 12%, 9%, 3% and 15%, the survival of Lactobacillus acidophilus was 42.62%, 56.50%, 45.05% and 42.69% and the viable cells of lyophilized powder were 0.40×1011cfu/g,0.45×1011cfu/g, 0.52×1011 and 1.09×1011cfu/g, respectively.

Effect of Five Materials Including Sucrose, Lactose, Skim Milk, Yeast, Vitamin B2 on Survival of Lactobacillus bulgaricus during Freeze-Drying

2013 ◽  
Vol 700 ◽  
pp. 255-258 ◽  
Author(s):  
He Chen ◽  
Qiu Hong Zhang ◽  
Qian Luo ◽  
Guo Wei Shu
Keyword(s):  
Survival Rate ◽  
Freeze Drying ◽  
Skim Milk ◽  
Lactobacillus Bulgaricus ◽  
Vitamin B ◽  
Protective Agent ◽  
Survival Ratio ◽  
Minimal Impact ◽  
Viable Cells ◽  
Impact On Survival

effect of five materials including sucrose, lactose, skim milk, yeast, vitamin B2on survival rate ofLactobacillus bulgaricusduring freeze-drying was studied by measured survival and the number of viable cells. Designed different concentrations of these different protective agents, respectively, freeze-drying perfectly and then counted the survival ratio ofLactobacillus bulgaricusand the number of viable cells. Results were as follows: addition of skim milk and yeast had significant effects while addition of vitamin B2had minimal impact on survival rate ofLactobacillus bulgaricus. The optimum concentration of single protective agent ofLactobacillus bulgaricusduring freeze-drying was that 25% (W/V) sucrose, 20% (W/V) lactose, 25% (W/V) skim milk, 20% (W/V) yeast and 25% (W/V) vitamin B2, respectively, and survival rate was 24.5%, 35.6%, 64.4%, 62.2%, and 16.3%.

scholarly journals Optimization of compatible solutes for improving survival of freeze-dried Lactobacillus delbrueckii subsp. bulgaricus using Box-Behnken design

2021 ◽  
Vol 25 (2) ◽  
pp. 301-306
Author(s):  
Guowei Shu ◽  
Bohao Li ◽  
Meng Zhang ◽  
Jie Huang ◽  
Li Chen ◽  
...  
Keyword(s):  
Survival Rate ◽  
Industrial Development ◽  
Freeze Drying ◽  
Compatible Solutes ◽  
Lactobacillus Delbrueckii ◽  
Drying Process ◽  
Sodium Glutamate ◽  
Box Behnken Design ◽  
Freeze Dried ◽  
Lactobacillus Delbrueckii Subsp

Abstract Lactobacillus delbrueckii subsp. bulgaricus is widely used in yogurt as a starter. The freeze-drying process may cause bacteria death. In the present work, the effect of three solutes (NaCl, sorbitol, and sodium glutamate) in MRS on viability of L.bulgaricus during freeze-drying was investigated. The optimal combination of adequate solutes was chosen by Box-Behnken Design. The survival rate and viable counts in freeze-dried powder, as well as the viable counts in broth, were used as responses. The results revealed that the optimum combination of solutes in MRS broth were 0.50% NaCl, 0.19% sorbitol, and 0.06% sodium glutamate. Under these optimal conditions, the survival rate was 53.2±0.14%, the viable counts in freeze-dried powder was 8.51±0.23×1010 CFU/g, and the viable counts in broth was 6.05±0.19 ×108 CFU/mL, which were increased by 17.18%, 15.94%, and 17.31%, respectively, compared to the control. This research demonstrated the possibility of viability improvement of L.bulgaricus, which may provide a feasible reference for industrial development.

scholarly journals Response Surface Optimization of Lyoprotectant from Amino Acids and Salts for Bifidobacterium Bifidum During Vacuum Freeze-Drying

2017 ◽  
Vol 21 (2) ◽  
pp. 3-10
Author(s):  
Kangru Qi ◽  
He Chen ◽  
Hongchang Wan ◽  
Man Hu ◽  
Yuxi Wu
Keyword(s):  
Amino Acids ◽  
Ascorbic Acid ◽  
Survival Rate ◽  
Response Surface ◽  
Dairy Products ◽  
Freeze Drying ◽  
Bifidobacterium Bifidum ◽  
Control Group ◽  
Drying Method ◽  
Response Surface Optimization

Abstract High quality probiotic powder can lay the foundation for the commercial production of functional dairy products. The freeze-drying method was used for the preservation of microorganisms, having a deleterious effect on the microorganisms viability. In order to reduce the damage to probiotics and to improve the survival rate of probiotics during freeze-drying, the Response Surface Methodology (RSM) was adopted in this research to optimize lyoprotectant composed of amino acids (glycine, arginine) and salts (NaHCO3 and ascorbic acid). Probiotic used was Bifidobacterium bifidum BB01. The regression model (p<0.05) was obtained by Box–Behnken experiment design, indicating this model can evaluate the freeze-drying survival rate of B. bifidum BB01 under different lyoprotectants. The results indicated these concentrations as optimal (in W/V): glycine 4.5%, arginine 5.5%, NaHCO3 0.8% and ascorbic acid 2.3%, respectively. Under these optimal conditions, the survival rate of lyophilized powder of B. bifidum BB01 was significantly increased by 80.9% compared to the control group (6.9±0.62%), the results were agreement with the model prediction value (88.7%).

Sign in / Sign up

Export Citation Format

Share Document