Effects of Copper Stress on Sucrose Metabolism in Seed of Two Rumex dentatus L. Populations

2013 ◽  
Vol 726-731 ◽  
pp. 102-105
Author(s):  
Wu Xing Huang ◽  
Cong Ren ◽  
Jing Qing Gao
Keyword(s):  
Cell Wall ◽  
Reducing Sugars ◽  
Control Cell ◽  
Invertase Activity ◽  
Sucrose Metabolism ◽  
The Other ◽  
Copper Stress ◽  
Cell Wall Invertase ◽  
Vacuolar Invertase ◽  
Cu Stress

Two Rumex dentatus populations, one from copper (Cu) mine and the other from uncontaminated site, were studied for sucrose metabolism of seed under Cu stress. Cu treatment significantly inhibited vacuolar invertase activity in non-metallicolous population (NMP), while it in metallicolous population (MP) was markedly higher than control. At control, cell wall invertase activity in MP showed little difference from NMP. However, after Cu treatment, cell wall invertase activity in MP was significantly higher than NMP. Activity of N/A-Inv was not affected by population or Cu treatment. Compared with control, sucrose and reducing sugars contents in MP were increased by Cu treatment, but those in NMP was decreased. Cu concentration in seed of NMP was higher than MP under Cu stress. These results suggested less Cu transported to seed and efficient sucrose metabolism in seed of MP might be partly reasons why R. dentatus can successful reproduction at Cu enriched soils.

Roles played by invertase and gene expression in the development of the horn-shaped gall on leaves of Rhus chinensis

2017 ◽  
Vol 44 (12) ◽  
pp. 1160 ◽  
Author(s):  
Zhen-Yuan Ruan ◽  
Xiao-Ming Chen ◽  
Pu Yang ◽  
Bing-Yi Wang
Keyword(s):  
Cell Wall ◽  
Invertase Activity ◽  
Activity Level ◽  
Rapid Expansion ◽  
Sink Strength ◽  
Cell Wall Invertase ◽  
Vacuolar Invertase ◽  
Growing Period ◽  
Rhus Chinensis ◽  
Invertase Gene

The present study deals with the growth and development of the horn-shaped gall, which is induced by Schlechtendalia chinensis Bell. on leaves of Rhus chinensis Mill. The relationship between gall formers and their host plants was investigated by means of the activities of various invertases, the expressions of the cell wall invertase gene (INV2), and vacuolar invertase gene (INV3) during gall development. Our results show that the increase in the sink strength of the galls required cell wall invertase and vacuolar invertase, and that vacuolar invertase had a particular impact during the early development. In addition, vacuolar invertase activity was always significantly higher in galls than in leaves. However, ionically bound cell wall invertase showed a slightly significant increased activity level when compared with the leaves after galls had entered the fast growing period. This result indicates that vacuolar invertase is related to the rapid expansion of the galls, but ionically bound cell wall invertase is involved in the rapid growth of tissues. The enhanced activity of cell wall invertase and the expression of INV2 may be a plant response to a gall-induced stress. Cytoplasmic invertase that acts as a maintenance enzyme, or takes part in the production of secondary metabolites, was elevated when intracellular acid invertase activity decreased.

Gibberellin (GA3) Enhances Cell Wall Invertase Activity and mRNA Levels in Elongating Dwarf Pea (Pisum sativum) Shoots

1993 ◽  
Vol 154 (2) ◽  
pp. 280-289 ◽  
Author(s):  
Liu-Lai Wu ◽  
John P. Mitchell ◽  
Norman S. Cohn ◽  
Peter B. Kaufman
Keyword(s):  
Cell Wall ◽  
Pisum Sativum ◽  
Invertase Activity ◽  
Mrna Levels ◽  
Cell Wall Invertase

Cell wall invertase activity in cultured tobacco tissues

1982 ◽  
Vol 2 (1) ◽  
pp. 3-9 ◽  
Author(s):  
Hamako Obata-Sasamoto ◽  
Trevor A. Thorpe
Keyword(s):  
Cell Wall ◽  
Invertase Activity ◽  
Cell Wall Invertase

An improved method for the measurement of cell wall invertase activity in sugarcane tissue

2001 ◽  
Vol 28 (4) ◽  
pp. 323 ◽  
Author(s):  
Peter L. Albertson ◽  
Kirrily F. Peters ◽  
Christopher P. L. Grof
Keyword(s):  
Cell Wall ◽  
Invertase Activity ◽  
Tissue Cell ◽  
Cell Wall Invertase ◽  
Tissue Extracts ◽  
Crude Plant Extract ◽  
Saccharum Sp ◽  
Spurious Effect ◽  
Assay Methods

Extraction and assay methods were developed for the determination of both soluble and cell wall invertase activity in sugarcane (Saccharum sp.) from minimal (0.5 g) tissue. Cell wall invertase (CWI) was measured using a pellet mix procedure and the pH optima ranged between pH 3.2 and 3.6. The pH optima for the soluble invertases were 4.5 and 7.3 for soluble acid (SAI) and neutral (NI) invertase, respectively. At low pH, acid hydrolysis of sucrose was observed and its spurious effect on measured enzyme activity was removed by the inclusion of additional controls run in parallel, which lacked crude plant extract. Invertase activity was examined in sugarcane tissues of varying ages. In leaves and stem, the SAI activity was greatly reduced in mature tissue extracts. Similarly, the CWI activity was reduced in older leaves. In contrast, a less marked drop in NI activity was observed in extracts from old leaves and activity from stem extracts remained constant irrespective of tissue age. The role of SAI has been linked to growth and differentiation and these observations suggest that CWI may also be intrinsically involved in these processes. NI appears to have a housekeeping role in maintaining hexose concentrations within the cytosol.

Effects of Copper Stress on Nitrogen Metabolism in Metallicolous and Non-Metallicolous Populations of Rumex dentatus L.

2013 ◽  
Vol 726-731 ◽  
pp. 98-101
Author(s):  
Wu Xing Huang ◽  
Yu Huang ◽  
Cong Ren
Keyword(s):  
Nitrogen Metabolism ◽  
Root Biomass ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
The Other ◽  
Copper Stress ◽  
Nitrate Content ◽  
Cu Stress ◽  
Leaves And Roots ◽  
Shoot And Root Biomass

Two Rumex dentatus populations, one from copper (Cu) mine and the other from uncontaminated site, were studied for plant growth, Cu accumulation and nitrogen metabolism under Cu stress. At control, nitrate content in leaves and roots of metallicolous population (MP) showed little difference from those of non-metallicolous population (NMP). At 10 μM Cu treatment, nitrate content in leaves and roots of MP were significantly higher than those of NMP. Cu treatments significantly reduced nitrate reductase activity in leaves and roots of NMP compared with the controls while not in MP. Shoot and root biomass of NMP were inhibited more significantly than those of MP. Cu concentration in shoot and root of NMP were higher than those of MP both at control and under Cu stress. These results suggested less Cu absorbed and efficient nitrogen metabolism in MP might be partly reasons why R. dentatus can colonize Cu enriched soils.

Regulation of invertase: a 'suite' of transcriptional and post-transcriptional mechanisms

2007 ◽  
Vol 34 (6) ◽  
pp. 499 ◽  
Author(s):  
Li-Fen Huang ◽  
Philip N. Bocock ◽  
John M. Davis ◽  
Karen E. Koch
Keyword(s):  
Cell Wall ◽  
Pectin Methylesterase ◽  
Invertase Activity ◽  
Kinase Domain ◽  
Sucrose Metabolism ◽  
Serine Threonine Kinase ◽  
Environmental Signals ◽  
Sensing Systems ◽  
C Terminus ◽  
Multiple Sensing

Recent evidence indicates that several mechanisms can alter invertase activity and, thus, affect sucrose metabolism and resource allocation in plants. One of these mechanisms is the compartmentalisation of at least some vacuolar invertases in precursor protease vesicles (PPV), where their retention could control timing of delivery to vacuoles and hence activity. PPV are small, ER-derived bodies that sequester a subset of vacuolar-bound proteins (such as invertases and protease precursors) releasing them to acid vacuoles in response to developmental or environmental signals. Another newly-identified effector of invertases is wall-associated kinase 2 (WAK2), which can regulate a specific vacuolar invertase in Arabidopsis (AtvacINV1) and alter root growth when osmolyte supplies are limiting. WAKs are ideally positioned to sense changes in the interface between the cell wall and plasma membrane (such as turgor), because the N-terminus of each WAK extends into the cell wall matrix (where a pectin association is hypothesised) and the C-terminus has a cytoplasmic serine/threonine kinase domain (signalling). Still other avenues of invertase control are provided by a diverse group of kinases and phosphatases, consistent with input from multiple sensing systems for sugars, pathogens, ABA and other hormones. Mechanisms of regulation may also vary for the contrasting sugar responses of different acid invertase transcripts. Some degree of hexokinase involvement and distinctive kinetics have been observed for the sugar-repressed invertases, but not for the more common, sugar-induced forms examined thus far. An additional means of regulation for invertase gene expression lies in the multiple DST (Down STream) elements of the 3′ untranslated region for the most rapidly repressed invertases. Similar sequences were initially identified in small auxin-up RNAs (SAUR) where they mediate rapid mRNA turnover. Finally, the invertase inhibitors, cell wall- and vacuolar inhibitors of fructosidase (CIF and VIF, respectively) are indistinguishable by sequence alone from pectin methylesterase inhibitors (PMEI); however, recent evidence suggests binding specificity may be determined by flexibility of a short, N-terminal region. These recently characterised processes increase the suite of regulatory mechanisms by which invertase – and, thus, sucrose metabolism and resource partitioning – can be altered in plants.

scholarly journals Transcriptomic and metabolomics responses to elevated cell wall invertase activity during tomato fruit set

2017 ◽  
Vol 68 (15) ◽  
pp. 4263-4279 ◽  
Author(s):  
Lei Ru ◽  
Sonia Osorio ◽  
Lu Wang ◽  
Alisdair R Fernie ◽  
John W Patrick ◽  
...  
Keyword(s):  
Cell Wall ◽  
Fruit Set ◽  
Tomato Fruit ◽  
Invertase Activity ◽  
Cell Wall Invertase
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