Determination of Sudan I by High Performance Liquid Chromatography with Immuno-Affinity Column Clean-Up

2013 ◽  
Vol 781-784 ◽  
pp. 1694-1699
Author(s):  
Li Xin Zhu ◽  
Wei Meng ◽  
Xue Mei Qiu ◽  
Ren Rong Liu ◽  
Ling Xu ◽  
...  
Keyword(s):  
High Performance ◽  
Aminocaproic Acid ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Affinity Column ◽  
Liquid Chromatographic Method ◽  
Sudan I ◽  
Carbodiimide Method ◽  
Chili Powder ◽  
Liquid Chromatographic

To prepare an immuno-affinity column (IAC) for Sudan I, Sepharose 4FF was activated by epichlorohydrin, and the density of epoxy is 80 μmol/g. Then it was coupled with 6-aminocaproic acid. The Sudan I immunoaffinity column was prepared by carbodiimide method in which modified Sepharose 4FF was used as carrier to couple with monoclonal antibody against Sudan I. A reversed-phase high performance liquid chromatographic method was used to determine the samples elution after the IAC cleaning up. Sudan I was determined by HPLC with a retention time of 5.82 min (flow rate of 1 ml/min). The column capacity is 1582.8 ng Sudan I when using 0.3 g Sepharose 4FF. Average recoveries of Sudan I from red chili powder spiked at levels of 0.25-3 mg/kg range from 44.52% to 77.40%. The coefficient of variation is 4.6% to 8.3%. The IAC method was found to be highly effective, sensitive and convenient for isolating the target analyte from sample.

DEVELOPMENT OF VALIDATED RP-HPLC METHOD FOR THE QUANTITATIVE ANALYSIS OF CHROMIUM PICOLINATE III IN A DIET VINEGAR FORMULATION

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (05) ◽  
pp. 48-52
Author(s):  
A Lodhi ◽  
◽  
A Jain ◽  
B. Biswal
Keyword(s):  
High Performance ◽  
Limit Of Detection ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Hplc Method ◽  
Chromium Picolinate ◽  
Limit Of Quantification ◽  
Pharmaceutical Dosage Forms ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

A validated high performance liquid chromatographic method was developed for the determination of chromium picolinate in pharmaceutical dosage forms. The analysis was performed at room temperature using a reversed-phase ODS, 5µm (250×4.6) mm column. The mobile phase consisted of acetonitrile: buffer (60:40 V/V) at a flow rate of 0.5 mL/min. The PDA-detector was set at 264 nm. The developed method showed a good linear relationship in the concentration range from 1.5 – 12.5 µg/mL with a correlation coefficient from 0.999. The limit of detection and limit of quantification were 0.0540513 and 0.1637919 µg/mL respectively.

DEVELOPMENT AND VALIDATION OF A NEW RP HPLC METHOD FOR ROUTINE DETERMINATION OF TEA TREE OIL IN BULK AND COSMECEUTICAL FORMULATIONS

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (07) ◽  
pp. 43-49
Author(s):  
B.P. Manjula ◽  
V. G Joshi ◽  
Siddamsetty Ramachandra Setty ◽  
M Geetha ◽  
Keyword(s):  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Hplc Method ◽  
Tea Tree Oil ◽  
Tea Tree ◽  
Liquid Chromatographic Method ◽  
Development And Validation ◽  
Liquid Chromatographic

Tea tree oil, an active ingredient of skin, hair and nail care cosmeceuticals, has claims for topical antimicrobial, analgesic and anti-inflammatory activity. Its complex composition is governed by ISO 4730:2017. Terpinene-4-ol is the principal constituent of the oil (35% - 48%) followed by γ-terpinene (14% -28%), α-terpinene (6%-12%) and 1,8-cineole (≤15%). A reversed-phase, isocratic high performance liquid chromatographic method has been developed and validated for routine determination of tea tree oil based on1,8-cineole content in bulk and commercially available cosmeceuticals using C18 column, methanol-water (70:30 v/v) as mobile phase and flow rate of 1mL/min. UV detection was done at 200 nm. Linearity of the method was established for 20-100μL/mL (R2 = 0.9992) with LOD, LOQ values of 0.5594 μL/mL and 5.5941μL/mL respectively. The % RSD values for robustness and precision were <1% and recovery ranged between 99.09-102.96%. The method was successfully applied for determination of 1,8-cineole content in cosmeceuticals.

Specific determination of 3-methoxy-4-hydroxyphenylethylene glycol in urine by liquid chromatography with post-column reaction.

1988 ◽  
Vol 34 (1) ◽  
pp. 87-90 ◽  
Author(s):  
K Abe ◽  
R Konaka
Keyword(s):  
High Performance ◽  
Signal To Noise Ratio ◽  
High Performance Liquid Chromatographic ◽  
Reference Intervals ◽  
Reversed Phase ◽  
Sodium Metaperiodate ◽  
Liquid Chromatographic Method ◽  
Analytical Recovery ◽  
Liquid Chromatographic

Abstract We describe a "high-performance" liquid-chromatographic method for determining 3-methoxy-4-hydroxyphenylethylene glycol (MHPG) in human urine. MHPG is separated on a reversed-phase column with isocratic elution, oxidized with sodium metaperiodate, and its absorbance measured at 365 nm. This method shows higher specificity, less interference for MHPG than methods involving electrochemical or fluorescence detection. Post-column derivatization of MHPG with periodate yields vanillin. The detection limit (twice the signal-to-noise ratio) in urine samples was 0.08 mg/L. Mean analytical recovery was 72%. Within-assay and day-to-day CVs were 2.9% and 6.5%, respectively. Reference intervals for MHPG in 24-h urine from apparently healthy subjects were 0.85-3.24 mg/day for men and 0.63-2.20 mg/day for women. In terms of creatinine excretion, the respective reference intervals were 0.55-1.99 and 0.70-1.96 mg per gram of creatinine.

Sign in / Sign up

Export Citation Format

Share Document