Analysis of the Bacterial Community by 454 Pyrosequencing in Two Freshwater Lakes: Yangcheng Lake and Taihu Lake in China

2013 ◽  
Vol 788 ◽  
pp. 315-320
Author(s):  
Fei Bai ◽  
Si Yu Zeng ◽  
Dan Li ◽  
Yuan Li ◽  
Miao He
Keyword(s):  
Bacterial Community ◽  
Bacterial Community Structure ◽  
454 Pyrosequencing ◽  
Taihu Lake ◽  
Harmful Algae ◽  
Freshwater Lakes ◽  
Algae Bloom ◽  
Operational Taxonomic Units ◽  
Pyrosequencing Method

Have a good understanding of the bacterial community structure, especially the cyanobacteria composition, works for clarifying the overall process of harmful algae bloom in freshwater lakes. In this study, the 454 pyrosequencing method was used to investigate the bacterial community in two typical freshwater lakes of China: Yangcheng Lake and Taihu Lake. A total of 30,663 16S rRNA reads were generated from 9 samples and assigned to 3,547 operational taxonomic units (OTUs) which represented 18 recognized phyla. For Yangcheng Lake, the dominant species in both July and early September were Proteobacteria, Actinobacteria, Bacteroidetes and Cyanobacteria. Taihu Lake had the same top three dominant phyla, but with smaller proportion of cyanobacteria. With regard to genus level of cyanobacteria, there were 5 genera which contained toxin-producing ones were identified in Yangcheng Lake, which manifested higher risk for long-term harmful algae injury. The constant level of cyanobacteria proportion from pre-bloom period to forthcoming period indicated that the proliferation of cyanobacteria quantity is more a sudden process not a gradual growth pattern. Only 3 genera were found in Taihu Lake and higher background amount of Microcystis in May of Taihu Lake made it more liable to suffer harmful algae bloom.

Soil remediation approach and bacterial community structure in a long-term contaminated soil by a mining spill (Aznalcóllar, Spain)

2021 ◽  
Vol 777 ◽  
pp. 145128
Author(s):  
M. Paniagua-López ◽  
M. Vela-Cano ◽  
D. Correa-Galeote ◽  
F. Martín-Peinado ◽  
F.J. Martínez Garzón ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Soil Remediation ◽  
Contaminated Soil ◽  
Bacterial Community Structure

Bacterial Community Structure in a Mollisol Under Long-Term Natural Restoration, Cropping, and Bare Fallow History Estimated by PCR-DGGE

Pedosphere ◽  
2009 ◽  
Vol 19 (2) ◽  
pp. 156-165 ◽  
Author(s):  
Guang-Hua WANG ◽  
Jian JIN ◽  
Jun-Jie LIU ◽  
Xue-Li CHEN ◽  
Ju-Dong LIU ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
Natural Restoration ◽  
Bare Fallow

Molecular Analysis of Bacterial Community Structure and Diversity in Unimproved and Improved Upland Grass Pastures

1999 ◽  
Vol 65 (4) ◽  
pp. 1721-1730 ◽  
Author(s):  
Allison E. McCaig ◽  
L. Anne Glover ◽  
James I. Prosser
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
Sequence Similarity ◽  
Soil Samples ◽  
Operational Taxonomic Units ◽  
16S Ribosomal Dna ◽  
Pcr Products ◽  
Evenness Indices ◽  
Low Coverage

ABSTRACT Bacterial community structure and diversity in rhizospheres in two types of grassland, distinguished by both plant species and fertilization regimen, were assessed by performing a 16S ribosomal DNA (rDNA) sequence analysis of DNAs extracted from triplicate soil plots. PCR products were cloned, and 45 to 48 clones from each of the six libraries were partially sequenced. Phylogenetic analysis of the resultant 275 clone sequences indicated that there was considerable variation in abundance in replicate unfertilized, unimproved soil samples and fertilized, improved soil samples but that there were no significant differences in the abundance of any phylogenetic group. Several clone sequences were identical in the 16S rDNA region analyzed, and the clones comprised eight pairs of duplicate clones and two sets of triplicate clones. Many clones were found to be most closely related to environmental clones obtained in other studies, although three clones were found to be identical to culturable species in databases. The clones were clustered into operational taxonomic units at a level of sequence similarity of >97% in order to quantify diversity. In all, 34 clusters containing two or more sequences were identified, and the largest group contained nine clones. A number of diversity, dominance, and evenness indices were calculated, and they all indicated that diversity was high, reflecting the low coverage of rDNA libraries achieved. Differences in diversity between sample types were not observed. Collector’s curves, however, indicated that there were differences in the underlying community structures; in particular, there was reduced diversity of organisms of the α subdivision of the class Proteobacteria (α-proteobacteria) in improved soils.

Changes in Bacterial Community Structure of Agricultural Land Due to Long-Term Organic and Chemical Amendments

2012 ◽  
Vol 64 (2) ◽  
pp. 450-460 ◽  
Author(s):  
Vasvi Chaudhry ◽  
Ateequr Rehman ◽  
Aradhana Mishra ◽  
Puneet Singh Chauhan ◽  
Chandra Shekhar Nautiyal
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
Agricultural Land

scholarly journals Effect of long-term herbicide applications on the bacterial community structure and function in an agricultural soil

2003 ◽  
Vol 46 (2) ◽  
pp. 139-146 ◽  
Author(s):  
Dave Seghers ◽  
Kristof Verthé ◽  
Dirk Reheul ◽  
Robert Bulcke ◽  
Steven D Siciliano ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
Agricultural Soil ◽  
Structure And Function ◽  
And Function

Effects of Long-Term Elevated CO2 on Rhizosphere and Bulk Soil Bacterial Community Structure in Pinus sylvestriformis Seedlings Fields

2011 ◽  
Vol 343-344 ◽  
pp. 351-356
Author(s):  
Xia Jia ◽  
Chun Juan Zhou
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Communities ◽  
Bacterial Community Structure ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Pinus Sylvestriformis ◽  
Soil Bacterial Community Structure ◽  
Gradient Gel Electrophoresis ◽  
Polymerase Chain

The effect of long-term elevated CO2(as open top chambers) on rhizosphere and bulk bacterial community structure in Pinus sylvestriformis seedlings field was investigated in July, August, and September. The bacterial communities were processed by Denaturing Gradient Gel Electrophoresis (DGGE) analysis of bacterial 16S rDNA fragments amplified by PCR (Polymerase Chain Reaction) from DNA extracted directly from soil. DGGE profiles from rhizosphere samples showed large changes in rhizosphere bacterial community under elevated CO2compared to ambient except for that in September. For bulk samples, bacterial community structure changed when exposed to elevated CO2in three months. With the exception of bulk samples in August, a similitude of bacterial communities structures existed between different elevated CO2concentrations by analyzing UPGMA dendrogram based on Jaccard’s coefficient.

scholarly journals Effect of formaldehyde exposure on bacterial communities in simulating indoor environments

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jianguo Guo ◽  
Yi Xiong ◽  
Taisheng Kang ◽  
Hua Zhu ◽  
Qiwen Yang ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Human Health ◽  
Bacterial Communities ◽  
Shannon Index ◽  
Chemical Degradation ◽  
Indoor Environments ◽  
Operational Taxonomic Units ◽  
Formaldehyde Exposure ◽  
Recommended Level

AbstractIndoor formaldehyde (CH2O) exceeding the recommended level is a severe threat to human health. Few studies have investigated its effect on indoor surface bacterial communities, affecting habitants' health. This study used 20-L glass containers to mimic the indoor environment with bacterial inputs from human oral respiration. The behavior of bacterial communities responding to CH2O varied among the different CH2O levels. The bacterial community structure significantly changed over time in the 0.054 mg·m−3 CH2O group, which varied from the 0.1 mg·m−3 and 0.25 mg·m−3 CH2O groups. The Chao1 and Shannon index significantly increased in the 0.054 mg·m−3 CH2O group at 6 week, while they remained unchanged in the 0.25 mg·m−3 CH2O group. At 12 week, the Chao1 significantly increased in the 0.25 mg·m−3 CH2O group, while it remained unchanged in the 0.054 mg·m−3 CH2O group. Only a few Operational Taxonomic Units (OTUs) significantly correlated with the CH2O concentration. CH2O-induced OTUs mainly belong to the Proteobacteria and Firmicutes. Furthermore, bacterial communities formed at 6 or 12 weeks differed significantly among different CH2O levels. Functional analysis of bacterial communities showed that inferred genes related to chemical degradation and diseases were the highest in the 0.25 mg·m−3 CH2O group at 12 weeks. The development of nematodes fed with bacteria collected at 12 weeks was applied to evaluate the bacterial community's hazards. This showed significantly impaired growth in the 0.1 mg·m−3 and 0.25 mg·m−3 CH2O groups. These findings confirmed that CH2O concentration and exposure time could affect the indoor bacterial community and formed bacterial communities with a possibly more significant hazard to human health after long-term exposure to high CH2O levels.

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