Determination of Picogram Levels of Levodopa in Pharmaceutical Preparations and Biofluids by Flow-Injection Chemiluminescence

2014 ◽  
Vol 884-885 ◽  
pp. 566-569 ◽  
Author(s):  
Yun Zhang ◽  
Su Ya Gao
Keyword(s):  
Flow Injection ◽  
Limit Of Detection ◽  
Pharmaceutical Preparations ◽  
Human Saliva ◽  
Quenching Effect ◽  
Dopa Concentration ◽  
Flow Injection Chemiluminescence ◽  
First Time ◽  
Serum And Urine

An ultrasensitive method for determination of L-dopa at picogram levels by flow-injection chemiluminescence (FI-CL) as presented for the first time, based on the quenching effect of levodopa (L-dopa) on the luminol-lysozyme reaction. It was found that the decrement of CL intensity was linearly proportional to the logarithm of L-dopa concentration ranging from 3.0 to 7.0 × 103pg mL-1(R= 0.9967), with the limit of detection (LOD) of 1.0 pg mL-1(3σ). The proposed procedure was successfully applied to the determination of L-dopa in pharmaceutical preparations, human saliva, serum, and urine samples with the recoveries ranging from 96.7% to 104.3% and RSDs less than 4.0% (n= 5).

scholarly journals Determination of Picogram Levels of Roxithromycin in Pharmaceutical, Human Serum, and Urine by Flow-Injection Chemiluminescence

2012 ◽  
Vol 2012 ◽  
pp. 1-5
Author(s):  
Jiangman Liu ◽  
Huan Yang ◽  
Yun Zhang ◽  
Min Wu ◽  
Haixiang Zhao ◽  
...  
Keyword(s):  
Human Serum ◽  
Flow Injection ◽  
Limit Of Detection ◽  
Inhibitory Effect ◽  
Injection System ◽  
Relative Standard ◽  
Flow Injection System ◽  
Flow Injection Chemiluminescence ◽  
Serum And Urine

A sensitive chemiluminescence (CL) method, based on the inhibitory effect of roxithromycin (ROX) on the CL reaction between luminol and dissolved oxygen in a flow-injection system, was first proposed for the determination of ROX at picogram levels. The decrement of CL intensity was linearly proportional to the logarithm of ROX concentrations ranging from 0.1 to 100 pg mL-1, giving the limit of detection (LOD) of 0.03 pg mL-1 (3σ). At a flow rate of 2.0 mL min-1, a complete analytical procedure including sampling and washing could be performed within 0.5 min, with relative standard deviations (RSDs) of less than 5.0% (n=5). The proposed procedure was applied successfully to the determination of ROX in pharmaceutical, human serum, and urine with the recoveries ranging from 90.0 to 110.0%.

Ultrasensitive Determination of Diphacinone by Flow Injection Chemiluminescence: Application to Quantification in Biofluids and Photodegradation Monitoring in Water Samples

2014 ◽  
Vol 97 (6) ◽  
pp. 1725-1729
Author(s):  
Xijuan Tan ◽  
Zhenghua Song ◽  
Donghua Chen ◽  
Hairu Lv
Keyword(s):  
Flow Injection ◽  
Water Samples ◽  
Continuous Monitoring ◽  
Simple Approach ◽  
Serum Samples ◽  
Experimental Conditions ◽  
Quenching Effect ◽  
Flow Injection Chemiluminescence ◽  
First Time

Abstract An ultrasensitive, quick, and simple approach for the determination of pg levels of diphacinone (DPN) by flow injection chemiluminescence (CL) analysis is proposed for the first time. It is based on the quenching effect of DPN on the CL intensity from a luminol–bovine serum albumin (BSA) CL system, for which the CL intensity decrease was linearly proportional to the logarithm of DPN concentration in the range of 5.0 to 5000 pg/mL. The LOD for DPN determination was as low as 2.0 pg/mL (3σ), and the RSD values were less than 5.0%. One determination cycle that included sampling and washing could be performed in 0.5 min with a sample throughput of 120/h under the optimum experimental conditions. This proposed method was successfully applied to determining DPN in human gastric juice and serum samples with recoveries from 91.8 to 114.3%, and to continuous monitoring of the degradation of DPN in water samples exposed to sunlight during 43 h with a variation ratio of 99.99%. The possible interaction behavior of BSA–DPN is briefly discussed.

scholarly journals Determination of Nanogram Quantities of Emodin in Pharmaceutical Preparations and Biofluids by Luminol-Myoglobin Chemiluminescence System

2012 ◽  
Vol 27 ◽  
pp. 73-81
Author(s):  
Yun Zhang ◽  
Xili He ◽  
Zhenghua Song
Keyword(s):  
Pharmaceutical Preparations ◽  
Serum Samples ◽  
Quenching Effect ◽  
Relative Standard ◽  
Complete Determination ◽  
Flow Injection Chemiluminescence ◽  
First Time ◽  
Nanogram Level ◽  
Sensitive Method

Based on the quenching effect of emodin on the luminol-myoglobin (Mb) reaction, a sensitive method for the determination of nanogram level emodin by flow injection chemiluminescence (FI-CL) is presented for the first time. It was found that the CL intensity from luminol-Mb system could be inhibited in the presence of emodin, and the decrement of CL intensity was linearly proportional to the logarithm of emodin concentration in the range of 0.5– (R= 0.9956) with the detection limit of (3σ). At a flow rate of , a complete determination of emodin, including sampling and washing, could be accomplished in 0.5 min with the relative standard deviations (RSDs) of less than 3.5% (n= 5). The proposed method was successfully applied to the determination of emodin in pharmaceutical preparations and human serum samples. The possible CL mechanism of luminol-Mb-emodin reaction was explained.

Determination of formaldehyde by flow injection analysis with spectrophotometric detection exploiting brilliant green–sulphite reaction

2015 ◽  
Vol 69 (6) ◽  
Author(s):  
Lúcio Bolognesi ◽  
Eder J. dos Santos ◽  
Gilberto Abate
Keyword(s):  
Flow Injection ◽  
Flow Injection Analysis ◽  
Brilliant Green ◽  
Limit Of Detection ◽  
Spectrophotometric Detection ◽  
Sample Throughput ◽  
First Time

AbstractA method for the determination of formaldehyde by flow injection analysis with spectrophotometric detection is proposed, based on retarding the reaction between brilliant green and sulphite by the addition of formaldehyde; this was investigated for formaldehyde quantification in extracts from wood-based panels. For the first time, a heating step was explored, providing a sample throughput of 50 analyses per hour, with a limit of detection of 0.02 mg L

Flow injection spectrofluorimetric determination of iron(III) in water using salicylic acid

2010 ◽  
Vol 64 (4) ◽  
Author(s):  
Adem Asan ◽  
Muberra Andac ◽  
Ibrahim Isildak
Keyword(s):  
Salicylic Acid ◽  
Flow Injection ◽  
Limit Of Detection ◽  
Sampling Rate ◽  
Linear Calibration ◽  
Fluorimetric Determination ◽  
Buffer Solution ◽  
Quenching Effect ◽  
Relative Standard

AbstractA simple and fast flow injection fluorescence quenching method for the determination of iron in water has been developed. Fluorimetric determination is based on the measurement of the quenching effect of iron on salicylic acid fluorescence. An emission peak of salicylic acid in aqueous solution occurs at 409 nm with excitation at 299 nm. The carrier solution used was 2 × 10−6 mol L−1 salicylic acid in 0.1 mol L−1 NH4+/NH3 buffer solution at pH 8.5. Linear calibration was obtained for 5–100 μg L−1 iron(III) and the relative standard deviation was 1.25 % (n = 5) for a 20 μL injection volume iron(III). The limit of detection was 0.3 μg L−1 and the sampling rate was 60 h−1. The effect of interferences from various metals and anions commonly present in water was also studied. The method was successfully applied to the determination of low levels of iron in real samples (river, sea, and spring waters).

scholarly journals Subnanogram Determination of Aniracetam in Pharmaceutical Preparations and Biofluids by Flow Injection Analysis with Chemiluminescence Detection Based on Its Enhancement of the Myoglobin-Luminol Reaction

2011 ◽  
Vol 94 (5) ◽  
pp. 1461-1466
Author(s):  
Shao Xiaodong ◽  
Li Ying ◽  
Li Fagen ◽  
Liu Yangqin ◽  
Song Zhenghua
Keyword(s):  
Flow Injection ◽  
Pharmaceutical Preparations ◽  
Serum Samples ◽  
Reaction Conditions ◽  
Whole Process ◽  
Optimum Reaction ◽  
Human Urine And Serum ◽  
Flow Injection Chemiluminescence ◽  
Urine And Serum

Abstract A novel flow injection chemiluminescence method with a myoglobin-luminol system is described for determining aniracetam. Myoglobin-bound aniracetam produced a complex that catalyzed the chemiluminescence reaction between luminol and myoglobin, leading to fast chemiluminescence. The chemiluminescence intensity in the presence of aniracetam was remarkably enhanced compared with that in the absence of aniracetam. Under the optimum reaction conditions the chemiluminescence increment produced was proportional to the concentration of aniracetam in the range of 0.1–1000.0 ng/mL (R2 = 0.9992), with a detection limit of 0.03 ng/mL (3δ). At a flow rate of 2.0 mL/min, the whole process, including sampling and washing, could be completed in 0.5 min, offering a sampling efficiency of 120/h; the RSD was less than 3.0% (n = 5). The method was satisfactory for determination of aniracetam in pharmaceutical preparations and human urine and serum samples. A possible mechanism of the reaction is also discussed.

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