scholarly journals Determination of Picogram Levels of Roxithromycin in Pharmaceutical, Human Serum, and Urine by Flow-Injection Chemiluminescence

2012 ◽  
Vol 2012 ◽  
pp. 1-5
Author(s):  
Jiangman Liu ◽  
Huan Yang ◽  
Yun Zhang ◽  
Min Wu ◽  
Haixiang Zhao ◽  
...  
Keyword(s):  
Human Serum ◽  
Flow Injection ◽  
Limit Of Detection ◽  
Inhibitory Effect ◽  
Injection System ◽  
Relative Standard ◽  
Flow Injection System ◽  
Flow Injection Chemiluminescence ◽  
Serum And Urine

A sensitive chemiluminescence (CL) method, based on the inhibitory effect of roxithromycin (ROX) on the CL reaction between luminol and dissolved oxygen in a flow-injection system, was first proposed for the determination of ROX at picogram levels. The decrement of CL intensity was linearly proportional to the logarithm of ROX concentrations ranging from 0.1 to 100 pg mL-1, giving the limit of detection (LOD) of 0.03 pg mL-1 (3σ). At a flow rate of 2.0 mL min-1, a complete analytical procedure including sampling and washing could be performed within 0.5 min, with relative standard deviations (RSDs) of less than 5.0% (n=5). The proposed procedure was applied successfully to the determination of ROX in pharmaceutical, human serum, and urine with the recoveries ranging from 90.0 to 110.0%.

scholarly journals Determination of Hesperetin in Pericarpium Citri Reticulatae and Human Serum Using Flow Injection Chemiluminescence

2012 ◽  
Vol 2012 ◽  
pp. 1-5
Author(s):  
Jiajia Wang ◽  
Xijuan Tan ◽  
Xin Li ◽  
Jiangman Liu ◽  
Zhenghua Song
Keyword(s):  
Dissolved Oxygen ◽  
Human Serum ◽  
Flow Injection ◽  
Inhibitory Effect ◽  
Injection System ◽  
Sampling Efficiency ◽  
Relative Standard ◽  
Flow Injection System ◽  
Flow Injection Chemiluminescence

A sensitive chemiluminescence (CL) method for the determination of hesperetin at nanogram levels was first presented. It was based on the inhibitory effect of hesperetin on luminol-dissolved oxygen CL reaction in a flow injection system. The decrements of CL intensity were logarithm over the concentrations of hesperetin in a range of 0.5 to 1000 ng mL−1, with a detection limit of 0.2 ng mL−1 (3σ) and the relative standard deviations lower than 2.1%. At a flow rate of 2.0 mL min−1, the whole determining performance including sampling and washing could be accomplished in 30 s, giving a sampling efficiency of 120 h−1. The proposed method was applied successfully to the direct hesperetin determination in human serum with recoveries from 97.0 to 106.6%, and indirect hesperidin quantification in 2.5 g pericarpium citri reticulatae showing that the content is 8.1±0.2%. The possible CL mechanism of luminol-dissolved oxygen-hesperetin reaction was also discussed in detail.

scholarly journals A novel online coupling of ion selective electrode with the flow injection system for the determination of vitamin B1

2016 ◽  
Vol 13 (2) ◽  
pp. 458-469
Author(s):  
Baghdad Science Journal
Keyword(s):  
Flow Injection ◽  
Dynamic Range ◽  
Limit Of Detection ◽  
Vitamin B1 ◽  
Pharmaceutical Formulations ◽  
Ion Selective Electrode ◽  
Injection System ◽  
Selective Electrode ◽  
Flow Injection System

A simple, fast, selective of a new flow injection analysis method coupled with potentiometric detection was used to determine vitamin B1 in pharmaceutical formulations via the prepared new selective membranes. Two electrodes were constructed for the determination of vitamin B1 based on the ion-pair vitamin B1-phosphotungestic acid (B1-PTA) in a poly (vinyl chloride) supported with a plasticized di-butyl phthalate (DBPH) and di-butyl phosphate (DBP). Applications of these ion selective electrodes for the determination of vitamin B1 in the pharmaceutical preparations for batch and flow injection systems were described. The ion selective membrane exhibited a near-Nernstian slope values 56.88 and 58.53 mV / decade, with the linear dynamic range of vitamin B1 was 5 x 10-5- 1 x 10-2 and 1 x 10-4-1 x 10-2 mol.L-1, in batch and FIA, respectively. The limit of detection was 3.5 x 10-5 and 9.5 x 10-5 mol.L-1, with the percentage linearity 98.85 and 95.22 in batch and FIA, respectively. The suggested ion selective electrode has been utilized perfection in the determination of vitamin B1 in pharmaceutical formulations using batch and flow injection system, respectively.

Determination of Picogram Levels of Levodopa in Pharmaceutical Preparations and Biofluids by Flow-Injection Chemiluminescence

2014 ◽  
Vol 884-885 ◽  
pp. 566-569 ◽  
Author(s):  
Yun Zhang ◽  
Su Ya Gao
Keyword(s):  
Flow Injection ◽  
Limit Of Detection ◽  
Pharmaceutical Preparations ◽  
Human Saliva ◽  
Quenching Effect ◽  
Dopa Concentration ◽  
Flow Injection Chemiluminescence ◽  
First Time ◽  
Serum And Urine

An ultrasensitive method for determination of L-dopa at picogram levels by flow-injection chemiluminescence (FI-CL) as presented for the first time, based on the quenching effect of levodopa (L-dopa) on the luminol-lysozyme reaction. It was found that the decrement of CL intensity was linearly proportional to the logarithm of L-dopa concentration ranging from 3.0 to 7.0 × 103pg mL-1(R= 0.9967), with the limit of detection (LOD) of 1.0 pg mL-1(3σ). The proposed procedure was successfully applied to the determination of L-dopa in pharmaceutical preparations, human saliva, serum, and urine samples with the recoveries ranging from 96.7% to 104.3% and RSDs less than 4.0% (n= 5).

scholarly journals Comparison of Uric Acid Quantity with Different Food in Human Urine by Flow Injection Chemiluminescence Analysis

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Jiajia Wang ◽  
Xijuan Tan ◽  
Zhenghua Song
Keyword(s):  
Uric Acid ◽  
Food Intake ◽  
Human Serum ◽  
Flow Injection ◽  
Human Urine ◽  
Inhibitory Effect ◽  
System A ◽  
Flow Injection Chemiluminescence ◽  
Sensitive Method

Based on the inhibitory effect of uric acid (UA) on luminol-Co2+chemiluminescence (CL) system, a sensitive method for the determination of UA at nanomolar level by flow injection (FI) CL was proposed. The proposed method was successfully applied to real-time monitoring of UA excretion in human 24 h urine with different food intake, showing that meats, vegetables, and porridge intake caused differential UA excretions of 879, 798, and 742 mg, respectively. It was also found that UA concentrations in urine under the three kinds of food intake simultaneously reached maximum at 2 h after meals with the values of 417, 318, and 288 μg mL−1, respectively. The UA concentration in human serum was also determined by this approach, and the possible mechanism of luminol-Co2+-UA CL reaction was discussed in detail.

scholarly journals Ultrasensitive determination for picomolar-level midecamycin in human serum by flow injection chemiluminescence using luminol–BSA system

2011 ◽  
Vol 26 (6) ◽  
pp. 349-355 ◽  
Author(s):  
Hairu Lv ◽  
Xijuan Tan ◽  
Yun Zhang ◽  
Zhenghua Song
Keyword(s):  
Standard Deviation ◽  
Detection Limit ◽  
Human Serum ◽  
Flow Rate ◽  
Flow Injection ◽  
Inhibitory Effect ◽  
Analysis Procedure ◽  
Serum Samples ◽  
Relative Standard ◽  
Flow Injection Chemiluminescence

An ultrasensitive method for determining picomolar midecamycin (MID) by flow injection (FI) chemiluminescence (CL) was first described based on the inhibitory effect of MID on luminol–BSA reaction. It was found that the CL intensity decrements were linear with the logarithm of MID concentrations in the range of 1.0–5000 pmol · l–1with a detection limit as low as 0.3 pmol · l–1(3σ). The relative standard deviation of seven repetitive measurements for 10 pmol · l–1MID was 3.0%. At a flow rate of 2.0 ml · min–1, the whole analysis procedure including sampling and washing could be finished in 30 s, offering the sample efficiency of 120 h–1. This proposed method was successfully applied to determine MID in human serum samples with the recoveries from 96.0 to 110.0%. The CL mechanism of luminol–BSA–MID reaction was also given.

scholarly journals Flow injection analysis of nitrogen dioxide using a galvanic detector

1998 ◽  
Vol 20 (1) ◽  
pp. 17-21
Author(s):  
Shan-jun Liu ◽  
Han-xi Shen ◽  
Jian-xing Feng ◽  
M. Tubino
Keyword(s):  
Nitrogen Dioxide ◽  
Flow Injection ◽  
Sample Volume ◽  
Injection System ◽  
Flow Monitoring ◽  
Relative Standard ◽  
Flow Injection System ◽  
Gaseous Sample ◽  
Monitoring Application

A flow injection configuration (FIA) based on a galvanic detector for the determination of nitrogen dioxide is described. The gaseous sample is directly injected into a gaseous carrier. The sample is transported toward the detector. The steady state measurements are not required to obtain the reproducible peak signals. The features of FIA are compared with that of continuous flow monitoring application. The flow injection system is simple, rapid and capable of detecting NO2in the range of 1-500 ppm (v/v). The measuring range and sensitivity of the galvanic detector in FIA depend on the sample volume. A relative standard deviation is 2.4%(n=10)for 200 ppm (v/v) of nitrogen dioxide. The sampling frequency is about 24 h-1

scholarly journals Automation of a flow injection system for the determination of dissolved silver at picomolar concentrations in seawater with inductively coupled plasma mass spectrometry

2003 ◽  
Vol 25 (4) ◽  
pp. 93-100 ◽  
Author(s):  
Jose L. Barriada ◽  
Jason B. Truscott ◽  
Eric P. Achterberg
Keyword(s):  
Mass Spectrometry ◽  
Flow Injection ◽  
Inductively Coupled Plasma ◽  
Limit Of Detection ◽  
Injection System ◽  
Inductively Coupled ◽  
Online Processing ◽  
Plasma Mass Spectrometry ◽  
Flow Injection System

An automated flow injection system for the determination of dissolved silver at ultratrace concentrations in seawater, and controlled under LabVIEW™, is described. The flow injection system allows online processing of seawater samples before their analysis using a magnetic sector inductively coupled plasma mass spectrometry (MS-ICP-MS) instrument. Samples were analysed with a minimum amount of manipulation, thereby reducing the risk of contamination. In addition, the flow injection approach with incorporation of an anion exchange minicolumn allowed ready removal of analytical interferences caused by the saline matrix. The software allowed full control of all flow injection components (valves and pumps) and removed manual time control and, therefore, operator errors. The optimized system was capable of five sample injections per h, including preconcentration and wash steps. The limit of detection was 0.5 pM for a 240-s sample load time, which allowed the determination of dissolved silver in open ocean waters, where picomolar concentration levels are typically encountered.

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