A Novel Living-Cell Imaging System Design with Ultra-Long Working Distance and High Numerical Aperture

2007 ◽  
Vol 364-366 ◽  
pp. 1077-1082
Author(s):  
Guo Liang Huang ◽  
Zhong Hua Dong ◽  
Cheng Deng ◽  
Shu Kuan Xu ◽  
Jiang Zhu ◽  
...  

Microscopy is an important tool in biology and medicine, but it is often limited to optical imaging structures with high numerical aperture(NA) with a short working distance(wd), for example NA = 0.6 and wd <1 mm are usual. The common microscope objective is inadequate for imaging of living cells in culture as an optical imaging structure with both high numerical aperture and long working distance is required. In this study, a novel optical design has been developed to meet the long working distance and high resolution power imaging of living cells in a vessel with a high culture solution thickness, where cells need to be developed in about 48 hours or a week. The developed optical design was characterized by an ultra-long working distance (wd >13.5 mm) and high numerical aperture (NA = 0.7). This optical imaging system is not only good for the subcellular imaging of free-floating cells in culture, but also for the imaging of cells attached at a surface of vessel.

2019 ◽  
Vol 28 (8) ◽  
pp. 084201
Author(s):  
Xin Liu ◽  
Jun Chang ◽  
Shuai Feng ◽  
Yu Mu ◽  
Xia Wang ◽  
...  

2016 ◽  
Vol 24 (4) ◽  
pp. 740-746
Author(s):  
徐明飞 XU Ming-fei ◽  
庞武斌 PANG Wu-bin ◽  
徐象如 XU Xiang-ru ◽  
王新华 WANG Xin-hua ◽  
黄玮 HUANG Wei

Photonics ◽  
2021 ◽  
Vol 8 (11) ◽  
pp. 476
Author(s):  
Shanshan Liang ◽  
Xinyu Li ◽  
Jiajing Kang ◽  
Mingming Wan ◽  
Jiahui Wang ◽  
...  

Peripheral retinal imaging is a unique approach for assessing and monitoring ocular diseases. In this paper, we proposed a design for an optical coherence tomography system to accomplish ultrawide field (>120°) retinal imaging without montages. Scanning of the sample arm was achieved via two ellipsoidal mirrors. The optical design software Zemax and an eye model were used to estimate the inherent aberrations in the system and the optical performance of retinal imaging. Simulation results of the aberrations in the designed system indicated that the designed system can achieve an unprecedented imaging field of view (FOV) while maintaining acceptable resolution without sacrificing the working distance. This work suggests that ultrawide field optical coherence tomography retinal imaging is achievable, which is highly important for the diagnosis and treatment of ocular—especially peripheral—retinopathy.


2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Lakhi Sharma ◽  
A. Roy ◽  
S. Panja ◽  
S. De

AbstractWe report an easy to construct imaging system that can resolve particles separated by $$\ge $$ ≥ 0.68 $$\upmu $$ μ m with minimum aberrations. Its first photon collecting lens is placed at a distance of 31.6 mm giving wide optical access. The microscope has a Numerical Aperture (NA) of 0.33, which is able to collect signal over 0.36 sr. The diffraction limited objective and magnifier recollects 77% photons into the central disc of the image with a transverse spherical aberration of 0.05 mm and magnification upto 238. The system has a depth of field of 142 $$\upmu $$ μ m and a field of view of 56 $$\upmu $$ μ m which images a large ensemble of atoms. The imaging system gives a diffraction limited performance over visible to near-infrared wavelengths on optimization of the working distance and the distance between the objective and magnifier.


2019 ◽  
Vol 48 (8) ◽  
pp. 814002
Author(s):  
毛姗姗 Mao Shanshan ◽  
李艳秋 Li Yanqiu ◽  
刘 克 Liu Ke ◽  
刘丽辉 Liu Lihui ◽  
郑 猛 Zheng Meng ◽  
...  

2007 ◽  
Vol 46 (6B) ◽  
pp. 3724-3728 ◽  
Author(s):  
Narak Choi ◽  
Seongbo Shim ◽  
Tom D. Milster ◽  
Jaisoon Kim

2009 ◽  
Vol 02 (01) ◽  
pp. 1-8 ◽  
Author(s):  
WATARU WATANABE ◽  
SACHIHIIRO MATSUNAGA ◽  
KIICHI FUKUI ◽  
KAZUYOSHI ITOH

Multiphoton absorption of femtosecond laser pulses focused through an objective with high numerical aperture (NA) can be used to image and manipulate cellular and intracellular objects. This review highlights recent advances in intracellular manipulation, including nanosurgery and labeling in living cells with femtosecond lasers.


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