Gallium Mobilization in Soil by Bacterial Metallophores

2017 ◽  
Vol 262 ◽  
pp. 513-516 ◽  
Author(s):  
Rïngo Schwabe ◽  
Britta Obst ◽  
Marika Mehnert ◽  
Dirk Tischler ◽  
Oliver Wiche
Keyword(s):  
Solvent Extraction ◽  
Soil Bacteria ◽  
Paracoccus Denitrificans ◽  
Soil Matrix ◽  
Alternative Strategies ◽  
Sensor Development ◽  
Desferrioxamine B ◽  
Potential Source ◽  
Valuable Metals ◽  
Chelating Compounds

In the present study we explore the idea of biotechnologically produced metallophore mixtures as selective chelating compounds for economically valuable metals from various sources. A complex soil matrix with natural levels of metal mineralization was employed as a potential source of metals. We focused on gallium-chelating metallophore preparations of two soil bacteria (Gordonia rubripertincta CWB2 and Paracoccus denitrificans PD1222) which were compared to the commercially available desferrioxamine B (DFOB). As a reference, the binding of iron was analyzed. The herein described successful mobilization of metals such as gallium from soil provides first hints towards alternative strategies, such as phytomining, sensor development, or solvent extraction based on metallophores. The metallophore mixture produced by the strains showed best results at pH 8 and allowed to mobilize gallium about three times better as the pure commercially available DFOB.

Trace Metal Exposure of Soil Bacteria Depends on Their Position in the Soil Matrix

2005 ◽  
Vol 39 (16) ◽  
pp. 5927-5932 ◽  
Author(s):  
Åsgeir R. Almås ◽  
Jan Mulder ◽  
Lars R. Bakken
Keyword(s):  
Trace Metal ◽  
Soil Bacteria ◽  
Metal Exposure ◽  
Soil Matrix

scholarly journals Recovery of cobalt and molybdenum from consumed catalyst using hydrochloric acid

2021 ◽  
Vol 882 (1) ◽  
pp. 012004
Author(s):  
D K Sutama ◽  
A Prasetya ◽  
H T B M Petrus ◽  
W Astuti
Keyword(s):  
Particle Size ◽  
Hydrochloric Acid ◽  
Petroleum Refinery ◽  
Agitation Speed ◽  
Acid Solutions ◽  
Hydrometallurgical Process ◽  
Potential Source ◽  
Hydrochloric Acid Solutions ◽  
Valuable Metals ◽  
Leaching Experiment

Abstract Cobalt and molybdenum are valuable metals whose presence in nature is very limited. The consumed catalyst, which is abundantly available in the petroleum refinery industry, is a potential source of those metals. A hydrometallurgical process using acid as a leaching agent is usually used to extract and separate the metals more effectively. This method is considered capable of yielding recovery of a higher percentage of metal. In this study, hydrochloric acid solutions at various concentrations of 1.0, 1.5 and 2 M were used. The consumed catalyst was obtained from Pertamina Refinery Unit IV, Cilacap, Indonesia. Leaching experiment was carried out for 300 minutes and sampling was undertaken at 1, 3, 5, 15, 30, 90 and 300 minutes. The particle size and agitation speed were fixed at 200 mesh and 400 rpm. Samples of consumed catalyst were analyzed using EDXRF before the leaching process. Samples of solution were analyzed using ICP-EOS. Experimental results have shown that the recovery of cobalt and molybdenum increases with the increase of either concentration of hydrochloric acid or temperature. The highest recoveries in cobalt and molybdenum were 34.66% and 5.03%, respectively, obtained at a concentration of hydrochloric acid of 2 M and temperature 60°C.

On the Immobilization of Desferrioxamine-Like Siderophores for Selective Metal Binding

2017 ◽  
Vol 262 ◽  
pp. 517-520 ◽  
Author(s):  
Marlene Kirstin Anke ◽  
Katarzyna Szymańska ◽  
Rïngo Schwabe ◽  
Oliver Wiche ◽  
Dirk Tischler
Keyword(s):  
Metal Binding ◽  
Chelating Agent ◽  
Reversed Phase ◽  
Culture Broth ◽  
High Yield ◽  
Metal Chelators ◽  
Binding Studies ◽  
Desferrioxamine B ◽  
Covalently Linked ◽  
Chelating Compounds

Gordonia rubripertincta CWB2 produces hydroxamate-type siderophores. Therefore it was cultivated under iron limitation. Analytical reversed-phase HPLC allowed determining a single peak of ferric iron chelating compounds from culture broth. The elution profile and its absorbance spectrum were similar to those of desferrioxamine B. The latter is a commercial available metal chelating agent which is of interest for industries. We successfully developed an HPLC protocol to separate metal-free and metal-loaded desferrioxamines. Further, we aimed to increase the re-usability of desferrioxamines as metal chelators by immobilization on silica based carriers. The siderophores of strain CWB2 have been covalently linked to the carrier with a high yield (up to 95%). Metal binding studies demonstrated that metals can be bound to non-immobilized as well as to the covalently linked desferrioxamines.

scholarly journals Recovery of valuable metals from argon oxygen decarburization (AOD) dusts by leaching, filtration and solvent extraction

2013 ◽  
Vol 140 ◽  
pp. 181-189 ◽  
Author(s):  
Sami Virolainen ◽  
Riina Salmimies ◽  
Mehdi Hasan ◽  
Antti Häkkinen ◽  
Tuomo Sainio
Keyword(s):  
Solvent Extraction ◽  
Valuable Metals ◽  
Argon Oxygen Decarburization

Solvent extraction of coals during analytical solvent swelling. A potential source of error

1991 ◽  
Vol 5 (1) ◽  
pp. 57-59 ◽  
Author(s):  
John W. Larsen ◽  
Jane C. Cheng ◽  
Cheng Sheng Pan
Keyword(s):  
Solvent Extraction ◽  
Potential Source ◽  
Source Of Error ◽  
Solvent Swelling

scholarly journals Pseudomonas fluorescens Pirates both Ferrioxamine and Ferricoelichelin Siderophores from Streptomyces ambofaciens

2015 ◽  
Vol 81 (9) ◽  
pp. 3132-3141 ◽  
Author(s):  
Justine Galet ◽  
Aurélie Deveau ◽  
Laurence Hôtel ◽  
Pascale Frey-Klett ◽  
Pierre Leblond ◽  
...  
Keyword(s):  
Pseudomonas Fluorescens ◽  
Soil Bacteria ◽  
Driving Forces ◽  
Agar Medium ◽  
Iron Chelation ◽  
Biological Processes ◽  
Microbial Competition ◽  
Content Type ◽  
Desferrioxamine B ◽  
Streptomyces Ambofaciens

ABSTRACTIron is essential in many biological processes. However, its bioavailability is reduced in aerobic environments, such as soil. To overcome this limitation, microorganisms have developed different strategies, such as iron chelation by siderophores. Some bacteria have even gained the ability to detect and utilize xenosiderophores, i.e., siderophores produced by other organisms. We illustrate an example of such an interaction between two soil bacteria,Pseudomonas fluorescensstrain BBc6R8 andStreptomyces ambofaciensATCC 23877, which produce the siderophores pyoverdine and enantiopyochelin and the siderophores desferrioxamines B and E and coelichelin, respectively. During pairwise cultures on iron-limiting agar medium, no induction of siderophore synthesis byP. fluorescensBBc6R8 was observed in the presence ofS. ambofaciensATCC 23877. Cocultures with aStreptomycesmutant strain that produced either coelichelin or desferrioxamines, as well as culture in a medium supplemented with desferrioxamine B, resulted in the absence of pyoverdine production; however, culture with a double mutant deficient in desferrioxamines and coelichelin production did not. This strongly suggests thatP. fluorescensBBbc6R8 utilizes the ferrioxamines and ferricoelichelin produced byS. ambofaciensas xenosiderophores and therefore no longer activates the production of its own siderophores. A screening of a library ofP. fluorescensBBc6R8 mutants highlighted the involvement of the TonB-dependent receptor FoxA in this process: the expression offoxAand genes involved in the regulation of its biosynthesis was induced in the presence ofS. ambofaciens. In a competitive environment, such as soil, siderophore piracy could well be one of the driving forces that determine the outcome of microbial competition.

Sign in / Sign up

Export Citation Format

Share Document