scholarly journals Mass production of the nematode Acrobeloides longiuterus using Tribolium castaneum and artificial solid media

2021 ◽  
Vol 12 (1) ◽  
pp. 14
Author(s):  
N. Thiruchchelvan ◽  
G. Thirukkumaran ◽  
G. Mikunthan
2019 ◽  
Vol 4 (01) ◽  
pp. 84-88
Author(s):  
Rajendra Singh ◽  
Vijay Pratap Singh ◽  
Ajay Kumar ◽  
Joginder Singh ◽  
Ankit Kumar ◽  
...  

The present investigation entitled “Mass Production of entomopathogenic fungai Beauveria bassiana (Metsch.) on liquid and solid media” was carried out at Bio Control Laboratory, Department of Entomology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut during 2018-19. In all there were eight treatments including control replicated thrice in a randomized block design. The results obtained in the study are summarized. The effect of different substrates for the mass production of Beauveria bassiana spore/ml was significantly higher recorded. Data recorded on 7, 14, 21, 28, 35 after the day after inoculation of various treatments in the, T10 Savoured dextrose broth (SDB), was the best treatment by bringing down the B. Bassiana production up to (49.48) spore/ml in liquid medium during the 2018-19 years. The other treatments in order of spore producing was with T11 Potato dextrose broth (PDB) (38.52), in liquid medium and solid medium in the treatment and T8 Cowpea Vigna unguiculata + Molasses + 1% YE + 1.0 g Dextrose (28.73), followed by with T7 Black gram Vigna mungo + Molasses + 1% YE + 1.0 g Dextrose (27.52 spore/ml, T5 Cowpea Vigna unguiculata + Molasses + 1% YE (17.53 spore/ml), T4 Black gram Vigna mungo + Molasses + 1% YE (14.87 spore/ml), T2 Cowpea Vigna unguiculata + Molasses (10.50 spore/ml), T1 Black gram Vigna mungo + Molasses (8.35 spore/ml), T9 Gram Cicer aeritinum + Molasses + 1% YE + 1.0 g Dextrose (3.92 spore/ml), T6 Gram Cicer aeritinum + Molasses + 1% YE (2.85 spore/ml) and T3 Gram Cicer aeritinum + Molasses respectively.


2019 ◽  
Vol 42 ◽  
Author(s):  
Joseph A. Tainter ◽  
Temis G. Taylor

Abstract We question Baumard's underlying assumption that humans have a propensity to innovate. Affordable transportation and energy underpinned the Industrial Revolution, making mass production/consumption possible. Although we cannot accept Baumard's thesis on the Industrial Revolution, it may help explain why complexity and innovation increase rapidly in the context of abundant energy.


1988 ◽  
Vol 102 ◽  
pp. 129-132
Author(s):  
K.L. Baluja ◽  
K. Butler ◽  
J. Le Bourlot ◽  
C.J. Zeippen

SummaryUsing sophisticated computer programs and elaborate physical models, accurate radiative and collisional atomic data of astrophysical interest have been or are being calculated. The cases treated include radiative transitions between bound states in the 2p4and 2s2p5configurations of many ions in the oxygen isoelectronic sequence, the photoionisation of the ground state of neutral iron, the electron impact excitation of the fine-structure forbidden transitions within the 3p3ground configuration of CℓIII, Ar IV and K V, and the mass-production of radiative data for ions in the oxygen and fluorine isoelectronic sequences, as part of the international Opacity Project.


Author(s):  
K. Yoshida ◽  
F. Murata ◽  
S. Ohno ◽  
T. Nagata

IntroductionSeveral methods of mounting emulsion for radioautography at the electron microscopic level have been reported. From the viewpoint of quantitative radioautography, however, there are many critical problems in the procedure to produce radioautographs. For example, it is necessary to apply and develop emulsions in several experimental groups under an identical condition. Moreover, it is necessary to treat a lot of grids at the same time in the dark room for statistical analysis. Since the complicated process and technical difficulties in these procedures are inadequate to conduct a quantitative analysis of many radioautographs at once, many factors may bring about unexpected results. In order to improve these complicated procedures, a simplified dropping method for mass production of radioautographs under an identical condition was previously reported. However, this procedure was not completely satisfactory from the viewpoint of emulsion homogeneity. This paper reports another improved procedure employing wire loops.


Author(s):  
Karen S. Howard ◽  
H. D. Braymer ◽  
M. D. Socolofsky ◽  
S. A. Milligan

The recently isolated cell wall mutant slime X of Neurospora crassa was prepared for ultrastructural and morphological comparison with the cell wall mutant slime. The purpose of this article is to discuss the methods of preparation for TEM and SEM observations, as well as to make a preliminary comparison of the two mutants.TEM: Cells of the slime mutant were prepared for thin sectioning by the method of Bigger, et al. Slime X cells were prepared in the same manner with the following two exceptions: the cells were embedded in 3% agar prior to fixation and the buffered solutions contained 5% sucrose throughout the procedure.SEM: Two methods were used to prepare mutant and wild type Neurospora for the SEM. First, single colonies of mutant cells and small areas of wild type hyphae were cut from solid media and fixed with OSO4 vapors similar to the procedure used by Harris, et al. with one alteration. The cell-containing agar blocks were dehydrated by immersion in 2,2-dimethoxypropane (DMP).


Author(s):  
Jane Payne ◽  
Philip Coudron

This transmission electron microscopy (TEM) procedure was designed to examine a gram positive spore-forming bacillus in colony on various solid agar media with minimal artifact. Cellular morphology and organization of colonies embedded in Poly/Bed 812 resin (P/B) were studied. It is a modification of procedures used for undecalcified rat bone and Stomatococcus mucilaginosus.Cultures were fixed and processed at room temperature (RT) under a fume hood. Solutions were added with a Pasteur pipet and removed by gentle vacuum aspiration. Other equipment used is shown in Figure 3. Cultures were fixed for 17-18 h in 10-20 ml of RT 2% phosphate buffered glutaraldehyde (422 mosm/KgH2O) within 5 m after removal from the incubator. After 3 (30 m) changes in 0.15 M phosphate buffer (PB = 209-213 mosm/KgH2O, pH 7.39-7.41), colony cut-outs (CCO) were made with a scalpel.


1935 ◽  
Vol 14 (8) ◽  
pp. 434
Author(s):  
Bailey ◽  
P.E. Biggar ◽  
Hood ◽  
Herbert Austin ◽  
T. Fraser ◽  
...  
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