Seasonal changes in seed reduction in lodgepole pine cones caused by feeding of Leptoglossus occidentalis (Hemiptera: Coreidae)

2006 ◽  
Vol 138 (6) ◽  
pp. 888-896 ◽  
Author(s):  
Ward B. Strong
Keyword(s):  
Adult Female ◽  
Pinus Contorta ◽  
Seed Set ◽  
Lodgepole Pine ◽  
Seed Orchard ◽  
Damage Potential ◽  
Leptoglossus Occidentalis ◽  
Pine Cones ◽  
Insect Exclusion ◽  
Filled Seeds

AbstractManagement of Leptoglossus occidentalis Heidemann in lodgepole pine (Pinus contorta var. latifolia Engelm.) seed orchards of British Columbia, Canada, would be improved with knowledge of its damage potential at different times of the growing season. Mesh insect-exclusion bags were placed over cones, and adults or nymphs of L. occidentalis were enclosed in different bags for 9 periods of 10 to 38 days between 6 May and 17 September 2004. Feeding by adult females between 6 May and 28 May reduced total extractable seeds, a result of ovule damage before fertilization. Reduction in the number of filled seeds per cone was highest between 6 May and 29 June, with each adult female reducing yield by approximately 1.7 seeds per day. Between 29 June and 10 August, each adult female reduced the number of filled seeds per cone (seed set) by 1.0 to 1.25 per day. Seed set reduction declined to approximately 0.25 filled seeds per adult female per day after 10 August. Third to fifth instars caused seed set reduction between 0.6 and 1.2 filled seeds per cone per nymph feeding day from 29 June to 10 August. Utilizing these findings would improve management of L. occidentalis in a commercial seed orchard setting.

Feeding by Leptoglossus occidentalis (Hemiptera: Coreidae) reduces seed set in lodgepole pine (Pinaceae)

2001 ◽  
Vol 133 (6) ◽  
pp. 857-865 ◽  
Author(s):  
Ward B. Strong ◽  
Sarah L. Bates ◽  
Michael U. Stoehr
Keyword(s):  
British Columbia ◽  
Pinus Contorta ◽  
Seed Set ◽  
Lodgepole Pine ◽  
Seed Orchards ◽  
Leptoglossus Occidentalis ◽  
Insect Exclusion ◽  
Filled Seeds ◽  
Water Treated Control

AbstractLow seed set is a serious problem in seed orchards of lodgepole pine (Pinus contorta var. latifolia Engelmann) in the southern interior of British Columbia. We tested the hypothesis that Leptoglossus occidentalis Heidemann is responsible for the low seed set. Cones enclosed in insect exclusion bags as part of a pollination experiment produced significantly more filled seeds per cone than cones that were not bagged. In a separate bagging experiment, cones that were enclosed with a L. occidentalis female and her progeny produced only about one filled seed per cone, compared with about 28 seeds in bagged control cones. Changes in microclimate associated with the use of bags did not appear to be responsible for the observed increase in seed set in bagged cones. Leptoglossus occidentalis was also excluded from trees using the insecticide fenvalerate. Cones on fenvalerate-treated trees produced > 11 filled seeds per cone, whereas water-treated (control) cones produced < 1.7 filled seeds. These data suggest that L. occidentalis should be considered a serious pest in lodgepole pine seed orchards.

Biochemical assays for identifying seeds of lodgepole pine and other conifers fed on by Leptoglossus occidentalis Heidemann (Hemiptera: Coreidae)

2001 ◽  
Vol 79 (11) ◽  
pp. 1349-1357 ◽  
Author(s):  
Cameron G Lait ◽  
Sarah L Bates ◽  
Karen K Morrissette ◽  
John H Borden ◽  
Allison R Kermode
Keyword(s):  
Salivary Gland ◽  
Pinus Ponderosa ◽  
Pinus Contorta ◽  
Storage Proteins ◽  
Biochemical Marker ◽  
Lodgepole Pine ◽  
Picea Sitchensis ◽  
Salivary Proteins ◽  
Pinus Monticola ◽  
Leptoglossus Occidentalis

Radiography is a valuable tool for assessing quality of conifer seeds, but it cannot differentiate between aborted seeds and seeds that have been emptied by western conifer seed bug (Leptoglossus occidentalis Heidemann) feeding. We tested three biochemical marker-based assays that were developed to identify L. occidentalis damage to seeds of Douglas-fir, Pseudostuga menziesii (Mirb.) Franco, for their use in lodgepole pine, Pinus contorta var. latifolia Engelmann. The three assays included measurement of storage protein reserve depletion, immunodetection of fragments of insoluble (crystalloid) storage proteins, and immunodetection of L. occidentalis salivary proteins. Aborted seeds contained significantly less soluble and insoluble protein than seeds that were fed on by L. occidentalis. Polyclonal antibodies raised against 11S globulin crystalloid proteins or L. occidentalis salivary gland extracts only immunoreacted with proteins in seeds exposed to L. occidentalis feeding. In a single-blind test, antibody raised against salivary-gland extracts correctly distinguished between undamaged full seeds, unfilled aborted seeds, and seeds fed on by L. occidentalis. Immunodetection of L. occidentalis salivary proteins was also performed on seeds of Abies amabilis Dougl. ex J. Forbes, Tsuga heterophylla (Raf.) Sarg., Picea sitchensis Bong (Carr.), Pinus ponderosa Lawson, and Pinus monticola Dougl. ex D. Don. For all species, immunoreactive polypeptides were only detected in seeds fed on by L. occidentalis. These biochemical marker-based techniques could help researchers and seed orchard managers estimate seed losses caused by L. occidentalis in commercial seed orchards and natural forest stands.Key words: Leptoglossus occidentalis, saliva, biochemical markers, polyclonal antibody, immunodetection, Pinus contorta.

scholarly journals Lodgepole Pine and White Spruce Germination: Effects of Stratification and Simulated Aging

2005 ◽  
Vol 54 (1-6) ◽  
pp. 138-144 ◽  
Author(s):  
Jodie Krakowski ◽  
Y. A. El-Kassaby
Keyword(s):  
Picea Glauca ◽  
Pinus Contorta ◽  
Lodgepole Pine ◽  
White Spruce ◽  
Seed Orchard ◽  
Ex Situ ◽  
Conservation Strategies ◽  
Germination Capacity ◽  
Term Storage ◽  
Germination Parameters

Abstract The effects of seed pre-treatment (stratification/prechilling) and simulated aging on germination parameters (germination capacity, speed and value and peak value) were evaluated for several seedlots originating from seed orchard clones of lodgepole pine (Pinus contorta var. latifolia DOUGL. ex LOUD.) and white spruce (Picea glauca (MOENCH) VOSS). Region of origin and stratification had little effect on white spruce, while stratification enhanced germination speed and completeness of lodgepole pine. Broad-sense heritability for germination parameters ranged from 70 to 97% (unstratified) and from 81 to 96% (stratified) for pine, and from 95 to 97% (unstratified) and from 93 to 97% (stratified) for spruce. Simulated aging (short-term storage at high temperature and relative humidity, approximating the physiological effects of long-term storage) resulted in rapid deterioration of white spruce, with very little germination after six days of aging. Lodgepole pine germination increased during the first several aging treatments relative to the control, but germination capacity decreased following twelve days of aging, and was very low after 18 days. White spruce was nondormant and responded primarily to moisture conditions, whereas pine was strongly influenced by maternal effects. These results can be incorporated for more efficacious nursery production practices for commercial reforestation seedling production as well as ex-situ gene conservation strategies.

Evaluation of mating dynamics in a lodgepole pine seed orchard using chloroplast DNA markers

2002 ◽  
Vol 32 (3) ◽  
pp. 469-476 ◽  
Author(s):  
Michael U Stoehr ◽  
Craig H Newton
Keyword(s):  
Chloroplast Dna ◽  
Pinus Contorta ◽  
Gene Diversity ◽  
Lodgepole Pine ◽  
Variable Number Tandem Repeat ◽  
Seed Orchard ◽  
Variable Number ◽  
Male Reproductive Success ◽  
Pollen Dynamics ◽  
Chloroplast Dna Markers

Chloroplast DNA (cpDNA) markers were used to evaluate pollen dynamics in an operational lodgepole pine (Pinus contorta Dougl. ex Loud. var. latifolia Engelm.) seed orchard. High levels of cpDNA differentiation were obtained using six primer pairs specific to simple sequence repeat (SSR) and variable number tandem repeat (VNTR) loci dispersed around the lodgepole pine chloroplast genome. In 69 orchard parents genotypes, 46 multilocus haplotypes were detected with 31 of these being unique (i.e., having only one orchard parent). The number of variants (alleles) per locus ranged from two to seven with gene diversity levels of 0.44-0.72. Evaluation of wind-pollinated seedlots of 15 selected clones using these markers indicated low levels of selfing (2%) and pollen contamination (5%) but showed evidence of relatively high levels of differential male reproductive success.

DISTRIBUTION AND IMPACT OF LEPTOGLOSSUS OCCIDENTALS HEIDEMANN (HEMIPTERA: COREIDAE) IN SEED ORCHARDS IN BRITISH COLUMBIA

1996 ◽  
Vol 128 (6) ◽  
pp. 1065-1076 ◽  
Author(s):  
Suzie E. Blatt ◽  
John H. Borden
Keyword(s):  
British Columbia ◽  
Seed Production ◽  
Pseudotsuga Menziesii ◽  
Pinus Contorta ◽  
Lodgepole Pine ◽  
Douglas Fir ◽  
Seed Orchards ◽  
Threshold Levels ◽  
Management Measures ◽  
Leptoglossus Occidentalis

AbstractLeptoglossus occidentalis Heidemann (Hemiptera: Coreidae) is considered to be an important cone and seed pest in seed orchards in British Columbia; however, no impact studies have been done in Canada. Surveys to determine the distribution of L. occidentalis were conducted over a 3-year period in three coastal seed orchards of Douglas-fir, Pseudotsuga menziesii (Mirb.) Franco, and over a 2-year period in two seed orchards of lodgepole pine, Pinus contorta var. latifolia Engelmann. Leptoglossus occidentalis was found to distribute itself in patches, coinciding with a clonal preference. Seed losses were assessed for 2 years in the coastal Douglas-fir orchards and for 1 year in one lodgepole pine orchard. The percentages of partially filled seed that could have been caused by feeding of L. occidentalis or by a number of other factors were < 5% in coastal Douglas-fir for both 1992 and 1993 and ~14% in lodgepole pine in 1993. Apparently because populations of L. occidentalis were low, there was no correlation between the percentage of partially filled seed and number of insects per tree. Except in unusual circumstances, management measures in Canada are judged not to be necessary as populations of L. occidentalis are consistently below the calculated threshold levels required to cause ≥5% loss in seed production.

Best of both worlds: hybrids of two commercially important pines (Pinus contorta × P. banksiana) combine increased growth potential and high drought tolerance

2021 ◽  
Author(s):  
Simon Bockstette ◽  
Raul de la Mata ◽  
Barb R. Thomas
Keyword(s):  
Pinus Contorta ◽  
Mountain Pine Beetle ◽  
Lodgepole Pine ◽  
Seed Orchard ◽  
Jack Pine ◽  
Snp Markers ◽  
Growth Potential ◽  
Pine Seedlings ◽  
Drought Resistant ◽  
Pine Hybrids

Over 18 million hectares of lodgepole pine forests have been affected by a massive mountain pine beetle outbreak in western Canada. The ensuing need for reforestation compels forest managers to deploy more drought-resistant stock in anticipation of a hotter and drier future climate. Recent studies suggest that naturally occurring lodgepole pine × jack pine hybrids may be more drought-resistant than pure lodgepole pine. We used single nucleotide polymorphism (SNP) markers to determine the level of jack pine introgression present in the region J lodgepole pine seed orchard, in northwest Alberta, and identified six hybrid genotypes with 10-18% jack pine introgression for further study. We compared the performance of open-pollinated seedlings of lodgepole, jack and hybrid pines under well-watered and drought conditions in a greenhouse experiment. Seedlings were subjected to repeated drought cycles, withholding water for either two or three weeks. We found that hybrid pines grew significantly faster than lodgepole pine under well-watered conditions while retaining the high tolerance to prolonged drought exhibited by lodgepole pine seedlings, a particularly beneficial combination of traits during the critical stage of establishment. We therefore suggest the deployment of hybrid lodgepole pine as a strategy to promote adaptation to hotter and drier future climates.

Post-pollination mechanisms promoting outcrossing in a self-fertile conifer, Thuja plicata (Cupressaceae)

2005 ◽  
Vol 83 (3) ◽  
pp. 335-342 ◽  
Author(s):  
Lisa M O'Connell ◽  
Kermit Ritland
Keyword(s):  
Mating System ◽  
Seed Set ◽  
Seed Orchard ◽  
Thuja Plicata ◽  
Enzyme Electrophoresis ◽  
Embryo Abortion ◽  
Western Redcedar ◽  
Pollination Mechanisms ◽  
Number Of Seeds ◽  
Filled Seeds

In conifers, polyembryony (multiple embryos within an ovule) may decrease the number of seeds lost to random embryo abortion, and (or) increase the proportion of outcrossed seeds if outcrossed embryos out-compete selfed ones. Western redcedar (Thuja plicata Don ex D. Donn, Cupressaceae) is a conifer with a mixed (selfed and outcrossed) mating system and high self-fertility with simple (archegonial) polyembryony. To test whether polyembryony can decrease seed abortion or the proportion of selfed seedlings, we conducted controlled pollinations in a seed orchard in southwestern British Columbia. Four trees received a total of 48 pollination treatments consisting of self, outcross, or mixtures of self and outcross pollen. Enzyme electrophoresis identified selfed seeds in the mixed pollen treatments. Reduction in the proportion of filled seeds (seed set) owing to selfing was approximately 30% for three of the trees and 93% for the fourth. Following mixed pollinations we did not observe an increase in seed set attributable to polyembryony. However, when trees received high ratios of self-pollen they produced fewer selfed seedlings than expected, suggesting embryo competition. The consequences of these results on the mating system of western redcedar are discussed.Key words: conifer, Cupressaceae, embryonic lethals, inbreeding depression, polyembryony, Thuja plicata.

Variation in seed set and proportions of outcrossed progeny with clones, crown position, and top pruning in a Douglas-fir seed orchard

1986 ◽  
Vol 16 (3) ◽  
pp. 502-507 ◽  
Author(s):  
S. K. Omi ◽  
W. T. Adams
Keyword(s):  
Maximum Likelihood ◽  
Seed Set ◽  
Seed Orchard ◽  
Douglas Fir ◽  
Crown Position ◽  
Maximum Likelihood Procedure ◽  
Clonal Variability ◽  
Allozyme Loci ◽  
Filled Seeds

The effects of clonal variability, crown position of cones, and top pruning on the proportions of viable outcrossed progeny [Formula: see text] and filled seeds (PF) were investigated in a 20-year-old Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) seed orchard. Estimates of [Formula: see text] were derived by means of a maximum likelihood procedure from data from 10 allozyme loci. Values for [Formula: see text] and PF were significantly heterogeneous (P < 0.05) among six selected clones for all pruning treatment x clone combinations. On the average, estimated values for [Formula: see text] and [Formula: see text] were greater in seeds collected from the upper crown [Formula: see text] than from the lower crown [Formula: see text]. Combined over crown positions, pruning appeared to have little effect on [Formula: see text], although the proportion of filled seeds was slightly lower in pruned ramets [Formula: see text] than in unpruned ramets [Formula: see text]. The overall frequency of viable selfs [Formula: see text] in the progeny of the six clones was 0.08.

Forest structure altered by mountain pine beetle outbreaks affects subsequent attack in a Wyoming lodgepole pine forest, USA

2011 ◽  
Vol 41 (12) ◽  
pp. 2403-2412 ◽  
Author(s):  
Daniel M. Kashian ◽  
Rebecca M. Jackson ◽  
Heather D. Lyons
Keyword(s):  
Pinus Contorta ◽  
Mountain Pine Beetle ◽  
Stand Structure ◽  
Lodgepole Pine ◽  
Climate Change Scenarios ◽  
Cone Production ◽  
Mountain Pine ◽  
Large Trees ◽  
Pine Beetle ◽  
The 1960S

Extensive outbreaks of the mountain pine beetle ( Dendroctonus ponderosae Hopkins) will alter the structure of many stands that will likely be attacked again before experiencing a stand-replacing fire. We examined a stand of lodgepole pine ( Pinus contorta var. latifolia Engelm. ex S. Watson) in Grand Teton National Park currently experiencing a moderate-level outbreak and previously attacked by mountain pine beetle in the 1960s. Consistent with published studies, tree diameter was the main predictor of beetle attack on a given tree, large trees were preferentially attacked, and tree vigor, age, and cone production were unimportant variables for beetle attack at epidemic levels. Small trees killed in the stand were killed based mainly on their proximity to large trees and were likely spatially aggregated with large trees as a result of the previous outbreak. We concluded that the driving factors of beetle attack and their spatial patterns are consistent across outbreak severities but that stand structure altered by the previous outbreak had implications for the current outbreaks in the same location. This study should catalyze additional research that examines how beetle-altered stand structure affects future outbreaks — an important priority for predicting their impacts under climate change scenarios that project increases in outbreak frequency and extent.

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