DNA barcodes reveal inconsistent species boundaries in Diplolepis rose gall wasps and their Periclistus inquilines (Hymenoptera: Cynipidae)

AbstractRose gall wasps, Diplolepis Geoffroy (Hymenoptera: Cynipidae), induce structurally distinct galls on wild roses (Rosa Linnaeus; Rosaceae), which provide gallers with food and shelter. These galls are attacked by a wide variety of micro-hymenopterans, including Periclistus Förster (Hymenoptera: Cynipidae), which act as inquilines. Both Diplolepis and Periclistus are difficult to distinguish based on adult morphology, instead the structural appearance of galls is often used to distinguish species. Using the mitochondrial gene cytochrome c oxidase subunit I, we tested the species boundaries and built phylogenies of both Diplolepis and Periclistus. The molecular results have largely supported the validity of species described in the literature, with notable exceptions in four species groups. Periclistus exhibits a divide between the Palaearctic and Nearctic clades, and ranges from specialists to generalists in terms of host specificity. While it is premature to enact any taxonomic changes without additional molecular markers, this incongruence between morphological and molecular data indicates these groups need taxonomic revision and gall morphology alone may be inadequate to delimit species.

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Phylogeny of Holarctic gall wasps of the genera Diplolepis and Periclistus (Hymenoptera: Cynipidae) based on DNA barcodes

10.1101/530949 ◽

2019 ◽

Author(s):

Y. Miles Zhang ◽

Zoltán László ◽

Chris Looney ◽

Avar-Lehel Dénes ◽

Robert H. Hanner ◽

...

Keyword(s):

Molecular Markers ◽

Mitochondrial Gene ◽

Taxonomic Revision ◽

Molecular Data ◽

Host Use ◽

Dna Barcodes ◽

Gall Wasps ◽

Taxonomic Changes ◽

Species Groups ◽

Morphological And Molecular Data

AbstractRose gall wasps Diplolepis induce structurally distinct galls on wild roses, which provide gallers with food and shelter. These galls are attacked by a wide variety of micro-hymenopterans including another cynipid Periclistus that act as inquilines. Both Diplolepis and Periclistus are difficult to distinguish based on adult morphology, instead the structural appearance of galls is often used to distinguish species. Using the mitochondrial gene COI, we built phylogenies of both Diplolepis and Periclistus, while also estimating the ancestral host use of the inducers. Our phylogeny recovered the monophyly of Diplolepis, which have likely diverged from single-or multi-chambered leaf gallers to other plant organs. Periclistus exhibits a divide between the Palearctic and Nearctic clades, and ranges from specialists to generalists in terms of host specificity. The molecular results have largely supported the validity of species described in the literature, with notable exceptions in four species groups. While it is premature to enact any taxonomic changes without additional molecular markers, this incongruence between morphological and molecular data indicates these groups need taxonomic revision and gall morphology alone may be inadequate to delimit species.

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Testing species limits of Eurytomidae (Hymenoptera) associated with galls induced by Diplolepis (Hymenoptera: Cynipidae) in Canada using an integrative approach

The Canadian Entomologist ◽

10.4039/tce.2013.70 ◽

2013 ◽

Vol 146 (3) ◽

pp. 321-334 ◽

Cited By ~ 8

Author(s):

Y. Miles Zhang ◽

Michael W. Gates ◽

Joseph D. Shorthouse

Keyword(s):

Mitochondrial Gene ◽

Molecular Data ◽

Integrative Taxonomy ◽

Integrative Approach ◽

Dna Barcodes ◽

Species Limits ◽

New Synonyms ◽

Biological Studies ◽

History Of ◽

Morphological And Molecular Data

AbstractStudies of insect host-parasitoid relationships are often confounded by the difficulties associated with species delimitation in taxonomically challenging groups. Eurytomidae (Hymenoptera) are common parasitoids associated with galls induced by Cynipidae (Hymenoptera) and are difficult to identify due to their small size, morphological conservatism, and unreliable published host records. This study tests the species limits of eurytomids associated with galls induced by Diplolepis Geoffroy (Hymenoptera: Cynipidae) in Canada using an integrative taxonomy approach including adult morphology, the mitochondrial gene cytochrome c oxidase I, host records, and geographical range. Incongruences between morphological and molecular data were found within the Eurytoma discordans Bugbee complex, as Eurytoma discordans, Eurytoma acuta Bugbee, and Eurytoma calcarea Bugbee were shown to be new synonyms. The results also revealed the presence of cryptic species within Eurytoma spongiosa Bugbee. Furthermore, issues that have impeded ecological and biological studies of eurytomids associated with rose galls such as host specificity and sex association were resolved using DNA barcodes, providing new insights into the evolutionary history of this difficult group.

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A revision of the subgenus Dudaica Strand of the genus Drosophila Fallén, with descriptions of six new species (Diptera, Drosophilidae)

ZooKeys ◽

10.3897/zookeys.781.27354 ◽

2018 ◽

Vol 781 ◽

pp. 19-50

Author(s):

Takehiro K. Katoh ◽

Guang Zhang ◽

Masanori J. Toda ◽

Awit Suwito ◽

Jian-Jun Gao

Keyword(s):

New Species ◽

Molecular Data ◽

Dna Barcodes ◽

Key To Species ◽

Morphological And Molecular Data

The subgenus Dudaica Strand of the genus Drosophila Fallén has been known to comprise only two species: Drosophila (Dudaica) senilis Duda, 1926 (recorded from Indonesia, Philippines, Vietnam, Bhutan, and India) and D.malayana (Takada, 1976) (recorded from Malaysia). In the present study, this subgenus is revised, with D.malayana redescribed and six new species discovered and described from China, Malaysia, and Indonesia: gracilipalpis Katoh & Gao, sp. n., puberula Katoh & Gao, sp. n., albipalpis Katoh, Toda & Gao, sp. n., qiongzhouensis Katoh & Gao, sp. n., orthophallata Katoh, Toda & Gao, sp. n., and dissimilis Katoh & Gao, sp. n. Both morphological and molecular data (DNA barcodes) are used to distinguish the above species. A key to species of this subgenus is provided.

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Morphological and molecular study of the torrent catfishes (Sisoridae: Glyptosterninae) of Bhutan including the description of five new species

Zootaxa ◽

10.11646/zootaxa.4476.1.5 ◽

2018 ◽

Vol 4476 (1) ◽

pp. 40

Author(s):

RYAN J. THONI ◽

DHAN B. GURUNG

Keyword(s):

New Species ◽

Mitochondrial Gene ◽

Nuclear Gene ◽

Molecular Study ◽

Molecular Data ◽

Cyt B ◽

Dichotomous Key ◽

Morphological And Molecular Data

Torrent catfishes of the subfamily Glyptosterninae from Bhutan are examined based on morphological and molecular data. Five new species are described: Creteuchiloglanis bumdelingensis sp. nov., Exostoma mangdechhuensis sp. nov., Parachiloglanis benjii sp. nov., P. dangmechhuensis sp. nov., and P. drukyulensis sp. nov. Molecular data derived from the mitochondrial gene Cyt b and the nuclear gene RAG2 recovered relationships within Parachiloglanis and the Glyptosterninae. A dichotomous key to the Glyptosterninae of Bhutan is provided.

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Phylogenetic relationships and classification of the Malagasy pseudoxyrhophiine snake genera Geodipsas and Compsophis based on morphological and molecular data

Zootaxa ◽

10.11646/zootaxa.1517.1.2 ◽

2007 ◽

Vol 1517 (1) ◽

pp. 53-62 ◽

Cited By ~ 3

Author(s):

FRANK GLAW ◽

ZOLTÁN T. NAGY ◽

MIGUEL VENCES

Keyword(s):

Dna Sequences ◽

Phylogenetic Relationships ◽

Close Relationships ◽

Original Description ◽

Molecular Data ◽

Morphological Data ◽

Molecular Phylogenetic ◽

Species Groups ◽

Morphological And Molecular Data

Based on a specimen found at Montagne d'Ambre in northern Madagascar morphologically agreeing with Compsophis albiventris Mocquard, 1894, we report on the rediscovery of this enigmatic snake genus and species and its molecular phylogenetic relationships. Compsophis albiventris, considered to be the only representative of its genus and unreported since its original description, bears strong morphological similarities to species of Geodipsas Boulenger, 1896. A molecular phylogeny based on DNA sequences of three mitochondrial and nuclear genes (complete cytochrome b, fragments of 16S rRNA and c-mos) in Compsophis albiventris and three Geodipsas species corroborated close relationships between C. albiventris and Geodipsas boulengeri, and showed that the genera Compsophis and Geodipsas together form a monophyletic unit. Despite the general similarities, morphological data and chromatic features support the existence of two species groups, corresponding to Compsophis and Geodipsas. We consequently consider Geodipsas as a subgenus of Compsophis and transfer all species currently in Geodipsas into the genus Compsophis.

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The Philodromus pulchellus-group in the Mediterranean: taxonomic revision, phylogenetic analysis and biogeography (Araneae:Philodromidae)

Invertebrate Systematics ◽

10.1071/is06014 ◽

2007 ◽

Vol 21 (1) ◽

pp. 39 ◽

Cited By ~ 9

Author(s):

Christoph Muster ◽

Robert Bosmans ◽

Konrad Thaler

Keyword(s):

Taxonomic Revision ◽

Molecular Data ◽

Species Group ◽

Western Mediterranean ◽

Mediterranean Origin ◽

Character Variation ◽

Nomina Dubia ◽

Morphological And Molecular Data ◽

Continuous Character

The Philodromus pulchellus species-group is defined and diagnosed. Eleven species are included, described or redescribed, keyed and illustrated: P. afroglaucinus, sp. nov. from Algeria; P. bistigma Simon, 1870, P. glaucinus Simon, 1870, P. lamellipalpis, sp. nov. from Algeria; P. medius O. P.-Cambridge, 1872; P. pardalis, sp. nov. from northern Africa and the Iberian peninsula; P. pulchellus Lucas, 1846, P. punctigerus O. P.-Cambridge, 1908, P. ruficapillus Simon, 1885, P. simoni de Mello-Leitão, 1929, and P. wunderlichi, sp. nov. from the western Canary Islands. The validity of P. bistigma and P. medius is re-established (formerly in synonymy with P. pulchellus); neotype and lectotype, respectively are newly designated. The following new synonymies are proposed: P. torquatus O. P.- Cambridge, 1908 = P. pulchellus; P. salinarum Denis, 1939 = P. glaucinus; P. glaucinoides Wunderlich, 1987 = P. punctigerus; P. marionschmidti (Schmidt, 1990) = P. pulchellus. Philodromus albopictus Simon, 1875 and P. rubidus Simon, 1870 are considered nomina dubia. Determination of phylogenetic relationships within the group is difficult owing to continuous character variation, resulting in partially incongruent reconstructions using morphological and molecular data (partial mitochondrial cytochrome c oxidase I gene). Dispersal vicariance analysis provides support for a western Mediterranean origin of the group.

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Morphological and molecular diversity in the freshwater crab Potamon ruttneri-P. gedrosianum species complex (Decapoda, Brachyura) indicate the need for taxonomic revision

Crustaceana ◽

10.1163/15685403-00003512 ◽

2016 ◽

Vol 89 (2) ◽

pp. 129-139 ◽

Cited By ~ 2

Author(s):

Alireza Keikhosravi ◽

Reza Naderloo ◽

Christoph D. Schubart

Keyword(s):

New Record ◽

Molecular Diversity ◽

Taxonomic Status ◽

Taxonomic Revision ◽

Molecular Data ◽

Valid Species ◽

Indus River ◽

Mitochondrial 16S Rdna ◽

Morphological And Molecular Data ◽

Incomplete Sampling

Many species and subspecies of Potamon have been described from the easternmost distribution of the genus in the western tributaries of the Indus River. Most of them were synonymised subsequently under the two names of currently valid species known from the region: Potamon gedrosianum Alcock, 1909 and Potamon ruttneri Pretzmann, 1962. Genetic and morphological information, based on mitochondrial 16S rDNA and the first male gonopod (G1), were gathered in the course of the present study. The corresponding results suggest the occurrence of four groups and question the taxonomic status of both species. We also revise the distribution range of both species, in particular that of P. gedrosianum, with a new record from Iran. Overall, the study reveals the need for a major revision using further morphological and molecular data. Because of the complexity of this necessary revision and the incomplete sampling, we here refrain from proposing any taxonomic conclusions.

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Cytogenetic analysis of Hypomasticus copelandii and H. steindachneri: relevance of cytotaxonomic markers in the Anostomidae family (Characiformes)

Comparative Cytogenetics ◽

10.3897/compcytogen.v15.i1.61957 ◽

2021 ◽

Vol 15 (1) ◽

pp. 65-76

Author(s):

Filipe Schitini Salgado ◽

Marina Souza Cunha ◽

Silvana Melo ◽

Jorge Abdala Dergam

Keyword(s):

Sex Chromosome ◽

5S Rdna ◽

Molecular Data ◽

Banding Patterns ◽

As Species ◽

Heteromorphic Sex Chromosomes ◽

Species Groups ◽

Phylogenetic Hypotheses ◽

Morphological And Molecular Data ◽

Species Specific

Recent phylogenetic hypotheses within Anostomidae, based on morphological and molecular data, resulted in the description of new genera (Megaleporinus Ramirez, Birindelli et Galetti, 2017) and the synonymization of others, such as the reallocation of Leporinus copelandii Steindachner, 1875 and Leporinus steindachneri Eigenmann, 1907 to Hypomasticus Borodin, 1929. Despite high levels of conservatism of the chromosomal macrostructure in this family, species groups have been corroborated using banding patterns and the presence of different sex chromosome systems. Due to the absence of cytogenetic studies in H. copelandii (Steindachner, 1875) and H. steindachneri (Eigenmann, 1907), the goal of this study was to characterize their karyotypes and investigate the presence/absence of sex chromosome systems using different repetitive DNA probes. Cytogenetic techniques included: Giemsa staining, Ag-NOR banding and FISH using 18S and 5S rDNA probes, as well as microsatellite probes (CA)15 and (GA)15. Both species had 2n = 54, absence of heteromorphic sex chromosomes, one chromosome pair bearing Ag-NOR, 18S and 5S rDNA regions. The (CA)15 and (GA)15 probes marked mainly the subtelomeric regions of all chromosomes and were useful as species-specific chromosomal markers. Our results underline that chromosomal macrostructure is congruent with higher systematic arrangements in Anostomidae, while microsatellite probes are informative about autapomorphic differences between species.

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Two new species of snail-eating snakes of the genus Tropidodipsas (Serpentes, Dipsadidae) from southern Mexico, with notes on related species

Herpetozoa ◽

10.3897/herpetozoa.34.e69176 ◽

2021 ◽

Vol 34 ◽

pp. 233-257

Author(s):

Christoph I. Grünwald ◽

Sarahi Toribio-Jiménez ◽

Carlos Montaño-Ruvalcaba ◽

Hector Franz-Chávez ◽

Miguel A. Peñaloza-Montaño ◽

...

Keyword(s):

New Species ◽

Molecular Phylogeny ◽

Molecular Data ◽

Species Group ◽

Conservation Priorities ◽

Southern Mexico ◽

Two New Species ◽

Taxonomic Changes ◽

Morphological And Molecular Data ◽

Mexican Species

We describe two new species of Tropidodipsas related to the T. fasciata species group as defined by Kofron (1987), and provide morphological and molecular data to support the novelty of both species. A partial molecular phylogeny of the Mexican species of snail-eating snakes (Serpentes, Dipsadidae) is presented, and we discuss evolutionary relationships as supported by our molecular results. We analyze specific relationships of the new species described herein with their closest relatives. We present a distribution map for all species of Tropidodipsas and include photographs of living individuals of each species. Finally, we discuss other taxonomic changes based on our molecular phylogeny as well as conservation priorities of the new species.

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Revision of the Afrotropical species of the hover fly genus Mesembrius Rondani (Diptera, Syrphidae) using morphological and molecular data

ZooKeys ◽

10.3897/zookeys.1046.57052 ◽

2021 ◽

Vol 1046 ◽

pp. 1-141

Author(s):

Kurt Jordaens ◽

Georg Goergen ◽

Jeffrey H. Skevington ◽

Scott Kelso ◽

Marc De Meyer

Keyword(s):

Dna Barcode ◽

Molecular Data ◽

Junior Synonym ◽

Dna Barcodes ◽

Identification Keys ◽

Males And Females ◽

Morphological And Molecular Data ◽

First Time

The Afrotropical representatives of the hover fly genus Mesembrius Rondani, 1857 (Diptera) are divided into two subgenera, namely Mesembrius s.s. and Vadonimyia Séguy, 1951 and, in this present work, the subgenus Mesembrius s.s. is revised. A total of 23 Mesembrius s.s. species are recognised for the Afrotropics. Known species are re-described and six species new to science are described: Mesembrius arcuatussp. nov., M. copelandisp. nov., M. longipilosussp. nov., M. sulcussp. nov., M. tibialissp. nov. and M. vockerothisp. nov. Mesembrius africanus (Verrall, 1898) is considered a junior synonym of M. senegalensis (Macquart, 1842), M. ctenifer Hull, 1941 a junior synonym of M. caffer (Loew, 1858), M. lagopus (Loew, 1869) a junior synonym of M. capensis (Macquart, 1842) and M. platytarsis Curran, 1929 a junior synonym of M. simplicipes Curran, 1929. The females of Mesembrius chapini Curran, 1939, M. rex Curran, 1927 and M. regulus (Hull, 1937) are described for the first time. Lectotypes are designated for Mesembrius caffer, M. capensis, M. cyanipennis (Bezzi, 1915), M. minor (Bezzi, 1915), M. senegalensis, M. strigilatus (Bezzi, 1912) and M. tarsatus (Bigot, 1883). Separate identification keys for males and females are presented. We obtained 236 DNA barcodes for 18 species. The relationships amongst the different Mesembrius species are briefly discussed, based on morphological and DNA barcode data.

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