Regulation of IFN-λ1 Promoter Activity (IFN-λ1/IL-29) in Human Airway Epithelial Cells

Exposure to zinc-laden particulate matter in ambient and occupational settings has been associated with proinflammatory responses in the lung. IL-8 is an important proinflammatory cytokine in the human lung and is induced in human airway epithelial cells exposed to zinc. In this study, we examined the cellular mechanisms responsible for Zn2+-induced IL-8 expression. Zn2+ stimulation resulted in pronounced increases in both IL-8 mRNA and protein expression in the human airway epithelial cell line (BEAS-2B). IL-8 promoter activity was significantly increased by Zn2+ exposure in BEAS-2B cells, indicating that Zn2+-induced IL-8 expression is transcriptionally mediated. Mutation of the activating protein (AP)-1 response element in an IL-8 promoter-enhanced green fluorescent protein construct reduced Zn2+-induced IL-8 promoter activity. Moreover, Zn2+ exposure of BEAS-2B cells induced the phosphorylation of the AP-1 proteins c-Fos and c-Jun. We observed that Zn2+ exposure induced the phosphorylation of ERK, JNK, and p38 MAPKs, whereas inhibition of ERK or JNK activity blocked IL-8 mRNA and protein expression in BEAS-2B cells treated with Zn2+. In addition, we investigated the role of protein tyrosine phosphatases in the activation of signaling by Zn2+. Zn2+ treatment inhibited ERK- and JNK-directed phosphatase activities in BEAS-2B cells. These results suggested that Zn2+-induced inhibition of phosphatase activity is an initiating event in MAPK and AP-1 activation that leads to enhanced IL-8 expression by human airway epithelial cells.

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Differential regulation of the transcriptomic and secretomic landscape of sensor and effector funtions of human airway epithelial cells

Pneumologie ◽

10.1055/s-0037-1619390 ◽

2018 ◽

Vol 72 (S 01) ◽

pp. S100-S101

Author(s):

R Lehmann ◽

M Müller ◽

TE Klassert ◽

D Driesch ◽

M Stock ◽

...

Keyword(s):

Epithelial Cells ◽

Airway Epithelial Cells ◽

Differential Regulation ◽

Airway Epithelial ◽

Human Airway ◽

Human Airway Epithelial Cells

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Faculty Opinions recommendation of Cystic fibrosis transmembrane conductance regulator is expressed in mucin granules from Calu-3 and primary human airway epithelial cells.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718017856.793479176 ◽

2013 ◽

Author(s):

Steven M Rowe ◽

Sammeta Vamsee Raju

Keyword(s):

Cystic Fibrosis ◽

Epithelial Cells ◽

Airway Epithelial Cells ◽

Transmembrane Conductance Regulator ◽

Airway Epithelial ◽

Transmembrane Conductance ◽

Human Airway ◽

Cystic Fibrosis Transmembrane ◽

Human Airway Epithelial Cells

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Faculty Opinions recommendation of SARS-CoV-2 receptor ACE2 is an interferon-stimulated gene in human airway epithelial cells and is detected in specific cell subsets across tissues.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.737831436.793574366 ◽

2020 ◽

Cited By ~ 1

Author(s):

Mitchell Grayson

Keyword(s):

Epithelial Cells ◽

Airway Epithelial Cells ◽

Specific Cell ◽

Airway Epithelial ◽

Human Airway ◽

Human Airway Epithelial Cells

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Polyhexamethylene guanidine phosphate, chloromethylisothiazolinone, and particulate matter are dispensable for stress granule formation in human airway epithelial cells

Animal Cells and Systems ◽

10.1080/19768354.2021.1931442 ◽

2021 ◽

pp. 1-6

Author(s):

Arnoldo Cambronero-Urena ◽

Sunkyung Choi ◽

Seri Choi ◽

Kee K. Kim ◽

Eun-Mi Kim

Keyword(s):

Particulate Matter ◽

Epithelial Cells ◽

Airway Epithelial Cells ◽

Stress Granule ◽

Airway Epithelial ◽

Granule Formation ◽

Human Airway ◽

Polyhexamethylene Guanidine ◽

Guanidine Phosphate ◽

Human Airway Epithelial Cells

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ICAM-1-independent adhesion of neutrophils to phorbol ester-stimulated human airway epithelial cells

AJP Lung Cellular and Molecular Physiology ◽

10.1152/ajplung.1999.277.3.l465 ◽

1999 ◽

Vol 277 (3) ◽

pp. L465-L471 ◽

Cited By ~ 3

Author(s):

Alessandro Celi ◽

Silvana Cianchetti ◽

Stefano Petruzzelli ◽

Stefano Carnevali ◽

Filomena Baliva ◽

...

Keyword(s):

Epithelial Cells ◽

Airway Epithelial Cells ◽

Bronchial Epithelial Cells ◽

Neutrophil Adhesion ◽

Late Time ◽

Airway Epithelial ◽

Bronchial Epithelial ◽

Human Airway ◽

Stimulation Of ◽

Human Airway Epithelial Cells

Intercellular adhesion molecule-1 (ICAM-1) is the only inducible adhesion receptor for neutrophils identified in bronchial epithelial cells. We stimulated human airway epithelial cells with various agonists to evaluate whether ICAM-1-independent adhesion mechanisms could be elicited. Phorbol 12-myristate 13-acetate (PMA) stimulation of cells of the alveolar cell line A549 caused a rapid, significant increase in neutrophil adhesion from 11 ± 3 to 49 ± 7% (SE). A significant increase from 17 ± 4 to 39 ± 6% was also observed for neutrophil adhesion to PMA-stimulated human bronchial epithelial cells in primary culture. Although ICAM-1 expression was upregulated by PMA at late time points, it was not affected at 10 min when neutrophil adhesion was already clearly enhanced. Antibodies to ICAM-1 had no effect on neutrophil adhesion. In contrast, antibodies to the leukocyte integrin β-chain CD18 totally inhibited the adhesion of neutrophils to PMA-stimulated epithelial cells. These results demonstrate that PMA stimulation of human airway epithelial cells causes an increase in neutrophil adhesion that is not dependent on ICAM-1 upregulation.

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Somatic cell hemoglobin modulates nitrogen oxide metabolism in the human airway epithelium

Scientific Reports ◽

10.1038/s41598-021-94782-5 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Nadzeya Marozkina ◽

Laura Smith ◽

Yi Zhao ◽

Joe Zein ◽

James F. Chmiel ◽

...

Keyword(s):

Gene Expression ◽

Epithelial Cells ◽

Nitrogen Oxides ◽

Airway Epithelium ◽

Airflow Obstruction ◽

Airway Epithelial Cells ◽

Airway Epithelial ◽

Human Airway ◽

Human Airway Epithelium ◽

Human Airway Epithelial Cells

AbstractEndothelial hemoglobin (Hb)α regulates endothelial nitric oxide synthase (eNOS) biochemistry. We hypothesized that Hb could also be expressed and biochemically active in the ciliated human airway epithelium. Primary human airway epithelial cells, cultured at air–liquid interface (ALI), were obtained by clinical airway brushings or from explanted lungs. Human airway Hb mRNA data were from publically available databases; or from RT-PCR. Hb proteins were identified by immunoprecipitation, immunoblot, immunohistochemistry, immunofluorescence and liquid chromatography- mass spectrometry. Viral vectors were used to alter Hbβ expression. Heme and nitrogen oxides were measured colorimetrically. Hb mRNA was expressed in human ciliated epithelial cells. Heme proteins (Hbα, β, and δ) were detected in ALI cultures by several methods. Higher levels of airway epithelial Hbβ gene expression were associated with lower FEV1 in asthma. Both Hbβ knockdown and overexpression affected cell morphology. Hbβ and eNOS were apically colocalized. Binding heme with CO decreased extracellular accumulation of nitrogen oxides. Human airway epithelial cells express Hb. Higher levels of Hbβ gene expression were associated with airflow obstruction. Hbβ and eNOS were colocalized in ciliated cells, and heme affected oxidation of the NOS product. Epithelial Hb expression may be relevant to human airways diseases.

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