scholarly journals Pfmdr1 gene polymorphism of Plasmodium falciparum isolates from asymptomatic individuals of Dienga, southeastern Gabon

2018 ◽  
Vol 03 (01) ◽  
Author(s):  
Irene Pegha-Moukandja ◽  
Lady Charlene Kouna ◽  
Sydney Maghendji-Nzdondo ◽  
Jean Claude Biteghe-Bi-Essone ◽  
Dominique Fatima Voumbo-Matoumona ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Gene Polymorphism ◽  
Pfmdr1 Gene ◽  
Asymptomatic Individuals

scholarly journals Efficacy of Artemisinin Base Combination Therapy and Genetic Diversity of Plasmodium falciparum from Uncomplicated Ma-laria Falciparum Patient in District of Pesawaran, Province of Lampung, Indonesia

2019 ◽  
Author(s):  
Jhons Fatriyadi SUWANDI ◽  
Widya ASMARA ◽  
Hari KUSNANTO ◽  
Din SYAFRUDDIN ◽  
Supargiyono SUPARGIYONO
Keyword(s):  
Plasmodium Falciparum ◽  
Genetic Variation ◽  
Falciparum Malaria ◽  
Health Centre ◽  
Sequencing Data ◽  
Single Nucleotide ◽  
Pfmdr1 Gene ◽  
Pcr Product ◽  
Nucleotide Mutation ◽  
Reverse Primer

Background: Malaria is an infectious disease caused by Plasmodium sp., that still prevalence in some part of Indonesia. District of Pesawaran is one of malaria endemic area in the Province of Lampung. The purpose of this study was to evaluate the efficacy of the ACT treatment in the District of Pesawaran Province of Lampung, Indonesia from Dec 2012 to Jul 2013 and the genetic variation of the Plasmodium falciparum also studied. Methods: This study was observational analytic study of falciparum malaria patients treated with ACT and primaquine (DHP-PQ and AAQ-PQ) at Hanura Primary Health Centre (Puskesmas). DNA isolation was done with QIAmp DNA Mini Kit. Amplification of PfMDR1, MSP1, and MSP2 genes was done with appropriate forward and reverse primer and procedures optimized first. PCR Product of PfMDR1 gene was prepared for sequencing. Data analysis was done with MEGA 6 software. Results: The results of this research are DHP-PQ effectiveness was still wellness among falciparum malaria patients in District of Pesawaran, Province of Lampung, Indonesia. There is Single-nucleotide mutation of N86Y of PfMDR1 gene. The dominant alleles found are MAD20 and 3D7 alleles with Multiplicity of Infection (MOI) are low. Conclusion: Therapy of DHP-PQ as an antimalarial falciparum in Pesawaran District, Lampung, Indonesia is still good. The genetic variation found was the SNP on the N86Y PfMDR1 gene, with dominant allele MAD20 and 3D7.

scholarly journals Analysis of Plasmodium falciparum Pfcrt and Pfmdr1 genes in parasite isolates from asymptomatic individuals in Southeast Nigeria 11 years after withdrawal of chloroquine

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Moses N. Ikegbunam ◽  
Charles N. Nkonganyi ◽  
Bolaji N. Thomas ◽  
Charles O. Esimone ◽  
Thirumalaisamy P. Velavan ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Pregnant Women ◽  
Direct Sequencing ◽  
Artemisinin Combination Therapy ◽  
First Line ◽  
Mutant Genotype ◽  
Current Prevalence ◽  
New Strategy ◽  
Asymptomatic Individuals ◽  
High Level

Abstract Background A reversal of chloroquine (CQ) resistance following a period of withdrawal has raised the possibility of its re-introduction. This study evaluated the current prevalence of Pfcrt and Pfmdr1 alleles in Plasmodium falciparum isolates, 11 years after CQ withdrawal in Southeast Nigeria. Methods Filter-paper blood samples were collected from 725 non-febrile individuals, comprising 250 children (≤ 12 years), 250 pregnant women and 225 other adults, between October 2014 and February 2015 in Nnewi town, Southeast Nigeria. Nested PCR followed by direct sequencing was employed for the genotyping of Pfcrt and Pfmdr1 genes. Results A total of 103 parasites-positive samples were recovered, comprising of 48 (19.20%) among children, 20 (20.00%) among pregnant women and 35 (15.50%) among other adults cohort. The frequency of the mutant genotype of Pfcrt 76T, 75E and 74I was 94.50% each. Parasite isolates from children had a frequency of 100% for mutant alleles in all Pfcrt codons while isolates from pregnant women and other adults had a frequency of 91% each in all codons. Haplotype distribution of pfcrt gene were 5.45, 0.00 and 76.37% for CVMNK, SVMNT and CVIET, respectively. For Pfmdr1 gene, the frequency of 86Y, 184F and 1246Y mutant alleles were 8.54, 29.27 and 3.66%, respectively. Amongst the Pfmdr1 haplotypes analysed, NFD had the highest frequency of 24.4%, followed by YFD at 6.10%. NYF and NYY occurred the least (1.20%). Conclusion The high level of Pfcrt mutations is suggestive of a sustained CQ pressure on P. falciparum isolates in the study area, despite the change of first line treatment from CQ to artemisinin combination therapy for 11 years. A new strategy to ensure the complete withdrawal of CQ from the country is recommended.

scholarly journals IL4 gene polymorphism and previous malaria experiences manipulate anti-Plasmodium falciparum antibody isotype profiles in complicated and uncomplicated malaria

2009 ◽  
Vol 8 (1) ◽  
pp. 286 ◽  
Author(s):  
Piyatida Tangteerawatana ◽  
Hedvig Perlmann ◽  
Masashi Hayano ◽  
Thareerat Kalambaheti ◽  
Marita Troye-Blomberg ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Gene Polymorphism ◽  
Uncomplicated Malaria ◽  
Antibody Isotype

scholarly journals Mefloquine resistance in Plasmodium falciparum and increased pfmdr1 gene copy number

The Lancet ◽  
2004 ◽  
Vol 364 (9432) ◽  
pp. 438-447 ◽  
Author(s):  
Ric N Price ◽  
Anne-Catrin Uhlemann ◽  
Alan Brockman ◽  
Rose McGready ◽  
Elizabeth Ashley ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Pfmdr1 Gene

Rapid Turnover of Plasmodium falciparum Populations in Asymptomatic Individuals Living in a High Transmission Area

1996 ◽  
Vol 54 (1) ◽  
pp. 18-26 ◽  
Author(s):  
Pierre Daubersies ◽  
Sebastien Magne ◽  
Thierry Fandeur ◽  
Odile Mercereau-Puijalon ◽  
Selma Sallenave-Sales ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Rapid Turnover ◽  
High Transmission ◽  
Transmission Area ◽  
Asymptomatic Individuals ◽  
High Transmission Area

scholarly journals Mutation in pfmdr1 gene in chloroquine-resistant Plasmodium falciparum isolates, Southeast Iran

2008 ◽  
Vol 12 (6) ◽  
pp. 630-634 ◽  
Author(s):  
Fatemeh Jalousian ◽  
Abdolhossein Dalimi ◽  
Siamak Mirab Samiee ◽  
Fatemeh Ghaffarifar ◽  
Faramarz Soleymanloo ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Pfmdr1 Gene

scholarly journals A P-glycoprotein homologue of Plasmodium falciparum is localized on the digestive vacuole.

1991 ◽  
Vol 113 (5) ◽  
pp. 1033-1042 ◽  
Author(s):  
A F Cowman ◽  
S Karcz ◽  
D Galatis ◽  
J G Culvenor
Keyword(s):  
Plasmodium Falciparum ◽  
Gene Copy Number ◽  
Chloroquine Resistance ◽  
Gene Copy ◽  
Striking Similarity ◽  
Multi Drug Resistance ◽  
Digestive Vacuole ◽  
Pfmdr1 Gene ◽  
P Glycoprotein ◽  
Quantitative Immunoblotting

Resistance to chloroquine in Plasmodium falciparum bears a striking similarity to the multi-drug resistance (MDR) phenotype of mammalian tumor cells which is mediated by overexpression of P-glycoprotein. We show here that the P. falciparum homologue of the P-glycoprotein (Pgh1) is a 160,000-D protein that is expressed throughout the asexual erythrocytic life cycle of the parasite. Quantitative immunoblotting analysis has shown that the protein is expressed at approximately equal levels in chloroquine resistant and sensitive isolates suggesting that overexpression of Pgh1 is not essential for chloroquine resistance. The chloroquine-resistant cloned line FAC8 however, does express approximately threefold more Pgh1 protein than other isolates which is most likely because of the increased pfmdr1 gene copy number present in this isolate. Immunofluorescence and immunoelectron microscopy has demonstrated that Pgh1 is localized on the membrane of the digestive vacuole of mature parasites. This subcellular localization suggests that Pgh1 may modulate intracellular chloroquine concentrations and has important implications for the normal physiological function of this protein.

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