scholarly journals Protective role of curcumin against the toxic effects of cyclophosphamide in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ)Bg9

2012 ◽  
Vol 2 (1) ◽  
pp. 2 ◽  
Author(s):  
Yasir Hasan Siddique
Keyword(s):  
Drosophila Melanogaster ◽  
Tissue Damage ◽  
Protective Role ◽  
Toxic Effects ◽  
Hsp70 Expression ◽  
The Third ◽  
Dose Dependent ◽  
Transgenic Drosophila ◽  
Third Instar Larvae

In the present study the effects of curcumin was studied against the toxic effects induced by 0.025 and 0.050 ml/ml of cyclophosphamide (CP) in the third instar larvae of transgenic <em>Drosophila melanogaster (hsp70-lacZ)Bg9</em> using hsp70 expression and dye exclusion test as a parameter. The exposure of the third instar larvae to 0.025ml/ml of CP along with 1, 5 and 10 mg/ml of curcumin results in the dose dependent significant decrease in the <em>hsp70</em> expression and tissue damage for 12, 24 and 48hr of duration. Similar results were obtained with the exposure of third instar larvae to 0.050 ml/ml of CP along with 1, 5 and 10 mg/ml of curcumin. The selected doses of curcumin i.e. 1, 5 and 10 mg/ml were not toxic but reduced significantly the expression of <em>hsp70</em> and tissue damage induced by CP. The results of the present study suggest that the curcumin has a protective role against the toxic effects of CP in the third instar larvae of transgenic <em>Drosophila melanogaster (hsp70-lacZ)Bg9</em>.

scholarly journals Protective role of Plumbago zeylanica extract against the toxic effects of ethinylestradiol in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ)Bg9 and cultured human peripheral blood lymphocytes

2011 ◽  
Vol 1 (1) ◽  
pp. 7 ◽  
Author(s):  
Yasir Hasan Siddique ◽  
Gulshan Ara ◽  
Mohammad Faisal ◽  
Mohammad Afzal
Keyword(s):  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Toxic Effects ◽  
Hsp70 Expression ◽  
Blood Lymphocytes ◽  
The Third ◽  
Human Peripheral Blood Lymphocytes ◽  
Transgenic Drosophila ◽  
Third Instar Larvae

In the present study the effects of Plumbago zeylanica extract were studied against the toxic effects induced by 10 μM of ethinylestradiol in cultured human peripheral blood lymphocytes using chromosomal aberrations as a parameter and in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ)Bg9 (by 0.50 and 1.0 μl/mL of ethinylestradiol) using hsp70 expression as a parameter. The selected doses of P. zeylanica extract i.e. 1.075x10–4, 2.125x10–4, 3.15x10–4 and 4.17x10–4 g/mL were not toxic but were potent enough to reduce significantly the toxic effects induced by ethinylestradiol in cultured human peripheral blood lymphocytes. The selected doses for the estimation of hsp70 expression in the third instar larvae of transgenic D. melanogaster (hsp70-lacZ) Bg9. i.e. 1, 2, 4 and 6 µl/mL were not toxic though reduced significantly the expression of hsp70 induced by 0.5 and1.0 µl/mL of ethinylestradiol.

Anti-inflammatory properties of probiotic bacteria on Salmonella-induced IL-8 synthesis in enterocyte-like Caco-2 cells

2010 ◽  
Vol 1 (2) ◽  
pp. 121-130 ◽  
Author(s):  
J. Malago ◽  
P. Tooten ◽  
J.F. Koninkx
Keyword(s):  
Tissue Damage ◽  
Protective Role ◽  
Bifidobacterium Bifidum ◽  
Probiotic Bacterium ◽  
Hsp70 Expression ◽  
Bacterial Cells ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Inflammatory Reactions ◽  
Mediated Reduction

Invasion of the gut by pathogenic Salmonella leads to production of IL-8 that initiates inflammatory reactions to combat the bacterium. However, its persistent production causes tissue damage and interventions that suppress IL-8 production prevent tissue damage. We hypothesised that probiotics could mediate their benefits via inhibition of IL-8 synthesis. Caco-2 cells were infected with probiotic Bifidobacterium infantis W52, Lactobacillus casei W56, Lactococcus lactis W58, Lactobacillus acidophilus W70, Bifidobacterium bifidum W23, or Lactobacillus salivarius W24 or pathogenic Salmonella enterica serovar Enteritidis 857 at 0, 0.2, 1, 2, 10, 20, 100 or 200 bacterial cells/Caco-2 cell for 1 hour. Cells were also exposed to a combination of one probiotic bacterium (200 bacterial cells/Caco-2 cell) and the graded numbers of Salmonella as either co-incubation (1 hour) or pre-incubation of the probiotic bacterium (1 hour) followed by Salmonella (1 hour). The cells recovered for 2 or 24 hours. IL-8 and Hsp70 were determined by ELISA and Western blot respectively. Both probiotics and Salmonella induced a dose- and time-dependent synthesis of IL-8 but probiotics induced far lower IL-8 levels than Salmonella. The Salmonella-induced IL-8 was significantly suppressed by B. infantis W52, L. casei W56 and L. lactis W58 at low numbers of Salmonella (0.2 to 20 bacterial cells/Caco-2 cell) and within 2 hours of recovery. The observed probiotic-mediated reduction in IL-8 secretion was transient, and lost after a few hours. In addition, these three probiotics induced a significant increase in Hsp70 expression while L. acidophilus W70, B. bifidum W23 and L. salivarius W24 induced a weak Hsp70 expression and could not suppress the Salmonella-induced IL-8 synthesis. We conclude that suppression of Salmonella-induced IL-8 synthesis by Caco-2 cells is exhibited by probiotics that induce expression of Hsp70, suggesting that the protective role of probiotics could be mediated, at least in part, via Hsp70 expression. This suppression is limited to a low number of infecting pathogenic Salmonella.

scholarly journals Evaluation of the toxic potential of arecoline toward the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg9

2018 ◽  
Vol 7 (3) ◽  
pp. 432-443 ◽  
Author(s):  
Barkha Shakya ◽  
Yasir Hasan Siddique
Keyword(s):  
Drosophila Melanogaster ◽  
Areca Nut ◽  
Carcinogenic Agent ◽  
The Third ◽  
Toxic Potential ◽  
Transgenic Drosophila ◽  
Third Instar Larvae

Arecoline is the key component of areca nut and has been suggested as a carcinogenic agent.

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