scholarly journals Diffuse cutaneous mastocytosis masquerading as linear IgA bullous dermatosis of childhood

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Tuntas Rayinda ◽  
Dyah Ayu Mira Oktarina ◽  
Retno Danarti
Keyword(s):  
Clinical Symptoms ◽  
Corticosteroid Treatment ◽  
Blue Staining ◽  
Toluidine Blue Staining ◽  
Bullous Dermatosis ◽  
Clinical Presentations ◽  
Linear Iga Bullous Dermatosis ◽  
Cutaneous Mastocytosis ◽  
Erythematous Plaques ◽  
Hematoxylin Eosin

Diffuse cutaneous mastocytosis is a rare form of cutaneous mastocytosis that can appear in heterogeneous clinical presentations, including eruption of papules, erythematous plaques, blisters, and erythroderma. We report a 1.5- year-old boy who presented with itchy wheals and blisters spreading on his body. The patient was initially managed as a linear IgA bullous dermatosis of childhood (LABD) because of the similarity of clinical symptoms and the presenting of linear IgA deposits at the basement membrane. Due to the development of urticarial plaque after the resolution of the blisters, the diagnosis of diffuse cutaneous mastocytosis was made based on clinical, histopathological (hematoxylin-eosin, Giemsa, and toluidine blue staining), and direct immunofluorescent examinations (IgA, IgG, IgM, C3). The symptoms were improved following antihistamines and oral corticosteroid treatment.

Non-Infectious Diseases of the Oral Soft Tissue: a New Approach

1993 ◽  
Vol 7 (2) ◽  
pp. 213-219 ◽  
Author(s):  
D.M. Williams
Keyword(s):  
Infectious Diseases ◽  
Oral Mucosa ◽  
Significant Proportion ◽  
Symptomatic Treatment ◽  
Mucous Membrane Pemphigoid ◽  
New Approach ◽  
Bullous Dermatosis ◽  
Clinical Presentations ◽  
Linear Iga Bullous Dermatosis ◽  
Oral Soft Tissue

A significant proportion of the non-infectious diseases of oral mucosa are either auto-immune in nature or have lesions which are the result of immunologically-mediated events. These include pemphigus, benign mucous membrane pemphigoid, linear IgA bullous dermatosis, dermatitis herpetiformis, epidermolysis bullosa acquisita, erythema multiforme, and lichen planus. Although each of these has certain specific characteristics, all may produce bullae, erosions, and ulcers on the oral mucosa, resulting in confusingly similar clinical presentations. With careful clinical, histological, and immunofluorescence examination, it is possible to establish a definitive diagnosis in a high proportion of cases. However, one of the most exciting developments which has emerged from recent research into these diseases is their precise molecular characterization. This raises the prospect of accurate, highly specific diagnostic tests which would provide the basis for sound clinical management, with original approaches replacing the somewhat unsatisfactory symptomatic treatment which is often all that is available.

scholarly journals Acupuncture Alleviates Menstrual Pain in Rat Model via Suppressing Eotaxin/CCR3 Axis to Weak EOS-MC Activation

2022 ◽  
Vol 2022 ◽  
pp. 1-13
Author(s):  
Wen-Yan Yu ◽  
Liang-Xiao Ma ◽  
Yuan Tian ◽  
Jie-Dan Mu ◽  
Zhou Zhang ◽  
...  
Keyword(s):  
Rat Model ◽  
Eosinophil Cationic Protein ◽  
Electrophysiological Recording ◽  
Blue Staining ◽  
Uterine Contractility ◽  
Menstrual Pain ◽  
Cationic Protein ◽  
Toluidine Blue Staining ◽  
Electrophysiological Method ◽  
Hematoxylin Eosin

Introduction. Emerging data show that chemokine-mediated inflammation is involved in the occurrence and maintenance of pain. Recent evidence suggests that eotaxin levels rise when dysmenorrhea happens. The purpose of this study is to investigate whether eotaxin/CC chemokine receptor 3 (CCR3) axis, a key regulatory pathway for eosinophils (EOS) recruitment, is involved in acupuncture analgesia for dysmenorrhea. Methods. After the cold congealing dysmenorrhea (CCD) rat model prepared, animals received perpendicular needling (PN) and transverse needling (TN) at SP6, respectively, for 20 min. The CCR3 agonist CCL11 was administered 30 min prior to acupuncture. Pain behavior was assessed via a writhing response. The uterine contraction test was detected by an electrophysiological method. Eotaxin, histamine (HIS), and interleukin-6 (IL-6) levels were evaluated by ELISA. The expression of CCR3 and histamine H1 receptor (H1R) was analyzed by RT-qPCR and Western blot. The expression of EOS, mast cells (MCs), eosinophil peroxidase (EPO), and eosinophil cationic protein (ECP) was assessed by hematoxylin-eosin staining (HE), Toluidine Blue staining (TB), and immunohistochemistry, respectively. Results. Acupuncture prominently attenuated the menstrual pain in CCD rats, particularly TN technique. Electrophysiological recording data showed that the increased uterine contractility was ameliorated by acupuncture. In addition, TN decreased the release of eotaxin, HIS, IL-6, and the expression of CCR3 and H1R. HE, TB staining, and immunohistochemistry experiments showed that the increased expression of EOS, MCs, EPO, and ECP in uterine tissues was reversed by TN. Furthermore, we found that the effects of TN against CCD-induced menstrual pain, increased ECP expression, and HIS level were abolished by CCL11. Conclusion. TN alleviated menstrual pain by improving the uterine inflammatory environment via suppressing eotaxin/CCR3 axis to weak EOS-MC activation in CCD rats. The study findings support the acupuncture as a promising approach for dysmenorrhea, meanwhile, indicating the importance of performing appropriate needling technique.

scholarly journals Cutaneous mastocytosis in an infant: Case report and clinicopathological correlation

2021 ◽  
pp. 108-111
Author(s):  
Solanki Prithviraj Kishoresingh ◽  
Bhide Vijayshri ◽  
Gadage Vijaya ◽  
Kulkarni Vinay ◽  
Patki Anil
Keyword(s):  
Pediatric Population ◽  
Blue Staining ◽  
Serum Tryptase ◽  
Toluidine Blue Staining ◽  
Clonal Proliferation ◽  
Normal Limits ◽  
Organ Systems ◽  
Uncommon Condition ◽  
Metachromatic Granules ◽  
Cutaneous Mastocytosis

Mastocytosis is a disorder of clonal proliferation of the mast cells, which can be cutaneous or systemic. Abnormal mast cell infiltration comprising multifocal compact clusters or cohesive aggregates can affect one or more organ systems. Cutaneous mastocytosis is a relatively uncommon condition in the pediatric population. We report a case of 9 month infant presented with multiple papular and vesicular skin rashes since 6 months of age. On clinical examination Darier’s sign was negative. The serum tryptase levels were within normal limits. Clinical differential diagnoses were benign cephalic histiocytosis vs cutaneous mastocytosis. Skin biopsy revealed a mononuclear cell infiltrate in the papillary dermis reaching up to the dermo-epidermal junction. Toluidine blue staining highlighted the metachromatic granules. CD117, CD30 IHC stains were positive which confirmed the diagnosis of cutaneous mastocytosis. This case is presented to highlight the histomorphology and the special stains in cases of mastocytosis.

Linear IgA bullous dermatosis limited to the seborrhoeic regions

1996 ◽  
Vol 135 (6) ◽  
pp. 1006-1007 ◽  
Author(s):  
S.-I. Ansai ◽  
Y. Mitsuhashi
Keyword(s):  
Bullous Dermatosis ◽  
Linear Iga Bullous Dermatosis

scholarly journals Non-Celiac Gluten Sensitivity: A Challenging Diagnosis in Children with Abdominal Pain

2018 ◽  
Vol 73 (Suppl. 4) ◽  
pp. 39-46 ◽  
Author(s):  
Frank M. Ruemmele
Keyword(s):  
Abdominal Pain ◽  
Celiac Disease ◽  
Clinical Symptoms ◽  
Gluten Sensitivity ◽  
Gluten Free ◽  
Clear Cut ◽  
Clinical Presentations ◽  
New Findings ◽  
Three Phase ◽  
Gi Symptoms

Several disorders related to the ingestion of gluten are well recognized despite overlapping clinical presentations: celiac disease, an autoimmune enteropathy triggered by gluten ingestions in susceptible individuals, allergy to wheat, and more recently non-celiac gluten sensitivity (NCGS). While celiac disease and wheat allergy are well-known disorders with a clear-cut diagnosis based on clinical tests and biological parameters, NCGS is a more difficult diagnosis, especially in children with functional gastrointestinal (GI) complaints. NCGS is considered a syndrome of intestinal but also extraintestinal symptoms occurring within hours, but sometimes even after several days of gluten ingestion. In children, the leading symptoms of NCGS are abdominal pain and diarrhea, while extraintestinal symptoms are rare, in contrast to adult patients. No precise diagnostic test nor specific biomarkers exist, except a rather cumbersome three-phase gluten-exposure, gluten-free diet, followed by a blinded placebo-controlled gluten challenge with crossover to provoke symptoms elicited by gluten in a reproducible manner that disappear on gluten-free alimentation. Recent data indicate that the peptide part of wheat proteins is not necessarily the sole trigger of clinical symptoms. Mono- or oligosaccharides, such as fructan and other constituents of wheat, were able to provoke GI symptoms in clinical trials. These new findings indicate that the term gluten sensitivity is probably too restrictive. The incidence of NCGS was reported in the range of 1–10% in the general population and to increase steadily; however, most data are based on patients’ self-reported gluten intolerance or avoidance without a medically confirmed diagnosis. Treatment consists of gluten avoidance for at least several weeks or months. Patients with NCGS require regular reassessment for gluten tolerance allowing with time the reintroduction of increasing amounts of gluten.

Ultrafast Toluidine Blue Staining for Rapid On-Site Evaluation of Cytological Smears

2020 ◽  
Vol 64 (4) ◽  
pp. 375-377
Author(s):  
Ekkehard Hewer ◽  
Anja M. Schmitt
Keyword(s):  
Organic Solvents ◽  
Toluidine Blue ◽  
Critical Factor ◽  
Blue Staining ◽  
Nuclear Morphology ◽  
Hazardous Chemicals ◽  
Toluidine Blue Staining ◽  
Site Evaluation ◽  
The Rose

Rapid on-site evaluation (ROSE) is one of cytopathology’s “unique selling propositions.” The quality, speed, and ease of handling of the staining used is a critical factor for the efficacy of the ROSE procedure. Here, we describe a modification of rapid toluidine blue staining that can be performed within 25 s, provides excellent nuclear morphology, and is compatible with subsequent Papanicolaou staining of the slides. Furthermore, exposure to hazardous chemicals is minimized, as no organic solvents other than the alcohol-based fixative and glycerin for temporary mounting and coverslipping are required. We have used this protocol successfully in our ROSE practice and have not observed any discrepancies between toluidine blue- and permanent Papanicolaou-stained slides.

scholarly journals Vancomycin‐induced linear IgA bullous dermatosis ( LABD )—an atypical presentation

2019 ◽  
Vol 7 (5) ◽  
pp. 1091-1093 ◽  
Author(s):  
Lorena Visentainer ◽  
Juliana Yumi Massuda ◽  
Maria Letícia Cintra ◽  
Renata Ferreira Magalhães
Keyword(s):  
Atypical Presentation ◽  
Bullous Dermatosis ◽  
Linear Iga Bullous Dermatosis
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