scholarly journals Meat juice serology for Toxoplasma gondii infection in chickens

2016 ◽  
Vol 5 (1) ◽  
Author(s):  
Alice Vismarra ◽  
Carlo Mangia ◽  
Elena Barilli ◽  
Franco Brindani ◽  
Cristina Bacci ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Serological Test ◽  
Epidemiological Studies ◽  
Heart Tissue ◽  
Sample Collection ◽  
Free Range ◽  
Meat Juice ◽  
Specific Pcr ◽  
Test Specificity ◽  
Toxoplasma Gondii Infection

<em>Toxoplasma gondii</em> is an important food-borne zoonoses. Free-range chickens are at particularly high risk of infection and are also excellent indicators of soil contamination by oocysts. In the present study, hearts of 77 free-range chickens were collected at slaughter. <em>T. gondii</em> meat juice ELISA was performed with a commercial kit, following validation with positive controls, from experimentally infected chickens, and negative ones. Out of 77 samples, only 66 gave sufficient meat juice for serology. Of these, 24 (36.4%) were positive for <em>T. gondii</em> considering the 5*SD values (calculated on the OD of negative controls) while all the samples were negative considering S/P% values. Parasite-specific PCR was carried out on all samples obtained from heart tissue and none were positive for the presence of T. gondii DNA. Results would suggest that further study on the use of meat juice with a validated serological test to detect <em>T. gondii</em> in chickens could lead to widespread epidemiological studies in this important intermediate host. However, sample collection and test specificity require further evaluation.

scholarly journals Detection of Viable Toxoplasma gondii in Free-Range Pigs from the Special Nature Reserve of Zasavica

2016 ◽  
Vol 65 (3-4) ◽  
pp. 1-6 ◽  
Author(s):  
Ljiljana Kuruca ◽  
Ivana Klun ◽  
Aleksandra Uzelac ◽  
Aleksandra Nikolić ◽  
Branko Bobić ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Nature Reserve ◽  
Heart Tissue ◽  
Human Infection ◽  
Serological Screening ◽  
Free Range ◽  
Modified Agglutination Test ◽  
Successful Isolation ◽  
Polymerase Chain ◽  
Great Appreciation

SummaryToxoplasma gondii is considered one of the most successful parasites of humans and animals. The ingestion of viable cysts through the consumption of undercooked pork is recognized as a significant route of human infection with T. gondii. The aim of this study was to investigate the presence of viable parasite in tissues of free-range pigs from the Zasavica Special Nature Reserve. All pigs were of the Mangulica breed, raised in a traditional way. The serological screening was performed using a modified agglutination test (MAT). The isolation of viable T. gondii was attempted by a bioassay of pig heart tissue in mice, while the real-time polymerase chain reaction (qPCR) targeted at the 529 bp repetitive element of T. gondii was used to detect parasitic DNA in digested hearts. Specific antibodies were detected in 12 out of 18 pigs examined. The bioassay was performed for five MAT-positive and one MAT-negative pig, and a total of three isolates were obtained. qPCR was performed for all samples, including one MAT-negative sample that was not bioassayed. The presence of T. gondii DNA was confirmed in all hearts with a positive bioassay as well as in one originating from seropositive and one from seronegative pig whose hearts were not bioassayed. The successful isolation of viable cysts, presence of risk factors (such as older age at the time of slaughter) and increased contact with the environment, along with the great appreciation of Serbian consumers towards home-cured Mangulica’s meat, make this breed worthy of consideration as a potentially important reservoir of human infection.

A sero-epidemiological survey of Toxoplasma gondii infection in free-range and caged chickens in northeast China

2008 ◽  
Vol 158 (4) ◽  
pp. 360-363 ◽  
Author(s):  
Jibao Zhu ◽  
Jigang Yin ◽  
Yue Xiao ◽  
Ning Jiang ◽  
Johan Ankarlev ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Northeast China ◽  
Epidemiological Survey ◽  
Free Range ◽  
Toxoplasma Gondii Infection

scholarly journals Toxoplasma gondii infection in meat-producing small ruminants: Meat juice serology and genotyping

2020 ◽  
Vol 76 ◽  
pp. 102060 ◽  
Author(s):  
Alessia Libera Gazzonis ◽  
Sergio Aurelio Zanzani ◽  
Luca Villa ◽  
Maria Teresa Manfredi
Keyword(s):  
Toxoplasma Gondii ◽  
Small Ruminants ◽  
Meat Juice ◽  
Toxoplasma Gondii Infection

scholarly journals Prevalence and genotyping of Toxoplasma gondii in stray cats in Mashhad area, Iran.

2019 ◽  
Author(s):  
Majid Khodaverdi ◽  
Gholamreza Razmi
Keyword(s):  
Toxoplasma Gondii ◽  
Low Frequency ◽  
Positive Sample ◽  
Rflp Markers ◽  
Type Ii ◽  
Toxoplasma Infection ◽  
Fecal Samples ◽  
Specific Pcr ◽  
Stray Cats ◽  
Toxoplasma Gondii Infection

Abstract Background: Cats as a definitive host have an important role in the epidemiology of toxoplasmosis in humans and animals. The aim of the study was to determine the frequency of Toxoplasma gondii infection and isolate and identify the genotypes of T. gondii in stray cats in the Mashhad suburb. Methods: From April 2016 to August 2017, 175 fecal samples from stray cats and 31 brain samples from cats killed in driving accidents were collected. The fecal samples were examined by fecal flotation technique and T. gondii-specific PCR. The brain samples were investigated by T. gondii-specific PCR and consequently examined by mice bioassay. The DNA of T. gondii isolated was genotyped using SAG1, SAG2, SAG3, BTUB and GRA6 as PCR-restriction fragment length polymorphism (PCR-RFLP) markers. Results: In the present study, Toxoplasma-like oocysts were microscopically observed in 2.2% (4/175) fecal samples. The presence of Toxoplasma oocysts was confirmed in one microscopy-positive sample by PCR. In addition, T. gondii DNA was detected in 4% (7/175) microscopy-negative samples using PCR. T. gondii was isolated from one brain PCR-positive sample by mice bioassay. The isolate was avirulent and many T. gondii cysts were observed in mice brain. The isolate was successfully genotyped by PCR-RLFP analysis .The isolated genotyped was type II. Besides, eight Toxoplasma-positive fecal samples contained insufficient DNA and only amplified at SAG-3 locus in PCR. These samples were also showed type II pattern at this locus. Conclusions: Parasitological and molecular results showed low frequency of Toxoplasma infection in the stray cats, and identified the genotype of T. gondii isolate as type II, for the first time in Mashhad area, Khorasan Razavi Province.

scholarly journals Prevalence and genotyping of Toxoplasma gondii in stray cats in Mashhad area, Iran.

2019 ◽  
Author(s):  
Majid Khodaverdi ◽  
Gholamreza Razmi
Keyword(s):  
Toxoplasma Gondii ◽  
Low Frequency ◽  
Positive Sample ◽  
Rflp Markers ◽  
Type Ii ◽  
Toxoplasma Infection ◽  
Fecal Samples ◽  
Specific Pcr ◽  
Stray Cats ◽  
Toxoplasma Gondii Infection

Abstract Background: Cats as a definitive host have an important role in the epidemiology of toxoplasmosis in humans and animals. The aim of the study was to determine the frequency of Toxoplasma gondii infection and isolate and identify the genotypes of T. gondii in stray cats in Mashhad suburb. Methods: From April 2016 to August 2017, 175 fecal samples from stray cats and 31 brain samples from cats killed in driving accidents were collected. The fecal samples were examined by fecal flotation technique and T. gondii -specific PCR. The brain samples were investigated by T. gondii -specific PCR and consequently examined by mice bioassay. The DNA of T. gondii isolated was genotyped using SAG1, SAG2, SAG3, BTUB and GRA6 as PCR-restriction fragment length polymorphism (PCR-RFLP) markers. Results: In the present study, Toxoplasma -like oocysts were microscopically observed in 2.2% (4/175) fecal samples. The presence of Toxoplasma oocysts was confirmed in one microscopy-positive sample by PCR. In addition, T . gondii DNA was detected in 4% (7/175) microscopy-negative samples using PCR. T. gondii was isolated from one brain PCR-positive sample by mice bioassay. The isolate was avirulent and many T. gondii cysts were observed in mice brain. The isolate was successfully genotyped by PCR-RLFP analysis .The isolated genotyped was type II. Beside, eight Toxoplasma- positive fecal samples contained insufficient DNA and only amplified at SAG-3 locus in PCR. These samples were also showed type II pattern at this locus. Conclusions: Parasitological and molecular results showed low frequency of Toxoplasma infection in the stray cats, and identified the genotype of T. gondii isolate as type II, for the first time in Mashhad area, Khorasan Razavi Province.

Toxoplasma gondii infection in sentinel and free-range chickens from Argentina

2012 ◽  
Vol 184 (2-4) ◽  
pp. 116-121 ◽  
Author(s):  
G. Moré ◽  
P. Maksimov ◽  
L. Pardini ◽  
D.C. Herrmann ◽  
D. Bacigalupe ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Free Range ◽  
Toxoplasma Gondii Infection

Toxoplasma gondii Infection in Hunted Wild Boars (Sus scrofa): Heart Meat Juice as an Alternative Sample to Serum for the Detection of Antibodies

EcoHealth ◽  
2015 ◽  
Vol 12 (4) ◽  
pp. 685-688 ◽  
Author(s):  
Catarina Coelho ◽  
Ana Patrícia Lopes ◽  
João Rodrigo Mesquita ◽  
Luís Cardoso ◽  
Madalena Vieira-Pinto
Keyword(s):  
Toxoplasma Gondii ◽  
Sus Scrofa ◽  
Wild Boars ◽  
Meat Juice ◽  
Toxoplasma Gondii Infection

Seroprevalence and Risk Factors of Toxoplasma gondii Infection in Free-Range Chickens in Senegal, West Africa

2020 ◽  
Vol 20 (1) ◽  
pp. 15-21 ◽  
Author(s):  
Amedine Sarr ◽  
Lokman Galal ◽  
Farid Boumediene ◽  
Azra Hamidović ◽  
Marie-Laure Dardé ◽  
...  
Keyword(s):  
Risk Factors ◽  
Toxoplasma Gondii ◽  
West Africa ◽  
Free Range ◽  
Toxoplasma Gondii Infection

scholarly journals Prevalence and genotyping of Toxoplasma gondii in stray cats in Mashhad area, Iran.

2019 ◽  
Author(s):  
Majid Khodaverdi ◽  
Gholamreza Razmi
Keyword(s):  
Toxoplasma Gondii ◽  
Low Frequency ◽  
Positive Sample ◽  
Rflp Markers ◽  
Type Ii ◽  
Toxoplasma Infection ◽  
Fecal Samples ◽  
Specific Pcr ◽  
Stray Cats ◽  
Toxoplasma Gondii Infection

Abstract Background: Cats as a definitive host have an important role in the epidemiology of toxoplasmosis in humans and animals. The aim of the study was to determine the frequency of Toxoplasma gondii infection and isolate and identify the genotypes of T. gondii in stray cats in Mashhad suburb. Methods: From April 2016 to August 2017, 175 fecal samples from stray cats and 31 brain samples from cats killed in driving accidents were collected. The fecal samples were examined by fecal flotation technique and T. gondii-specific PCR. The brain samples were investigated by T. gondii-specific PCR and consequently examined by mice bioassay. The DNA of T. gondii isolated was genotyped using SAG1, SAG2, SAG3, BTUB and GRA6 as PCR-restriction fragment length polymorphism (PCR-RFLP) markers. Results: In the present study, Toxoplasma-like oocysts were microscopically observed in 2.2% (4/175) fecal samples. The presence of Toxoplasma oocysts was confirmed in one microscopy-positive sample by PCR. In addition, T. gondii DNA was detected in 4% (7/175) microscopy-negative samples using PCR. T. gondii was isolated from one brain PCR-positive sample by mice bioassay. The isolate was avirulent and many T. gondii cysts were observed in mice brain. The isolate was successfully genotyped by PCR-RLFP analysis .The isolated genotyped was type II. Beside, eight Toxoplasma-positive fecal samples contained insufficient DNA and only amplified at SAG-3 locus in PCR. These samples were also showed type II pattern at this locus. Conclusions: Parasitological and molecular results showed low frequency of Toxoplasma infection in the stray cats, and identified the genotype of T. gondii isolate as type II, for the first time in Mashhad area, Khorasan Razavi Province.

Seroprevalence and factors associated with Toxoplasma gondii infection in wild boar (Sus scrofa) in a Mediterranean island

2010 ◽  
Vol 138 (9) ◽  
pp. 1257-1266 ◽  
Author(s):  
C. RICHOMME ◽  
E. AFONSO ◽  
V. TOLON ◽  
C. DUCROT ◽  
L. HALOS ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Wild Boar ◽  
Sus Scrofa ◽  
First Year ◽  
Factors Affecting ◽  
Landscape Characteristics ◽  
Meat Juice ◽  
Modified Agglutination Test ◽  
Mediterranean Island ◽  
Toxoplasma Gondii Infection

SUMMARYKnowledge of the factors affecting the presence of Toxoplasma gondii in wildlife is limited. Here we analyse which local landscape characteristics are associated with the presence of toxoplasmosis in wild boar, Sus scrofa, on the island of Corsica, France. Meat juice samples from 1399 wild boars collected during two hunting seasons were tested for T. gondii antibodies using the modified agglutination test (titre 1:4). The overall seroprevalence was 0·55 (95% CI 0·50–0·59) for the first year and 0·33 (95% CI 0·29–0·35) for the second year. Seroprevalence varied according to age and county. At the county level, seropositivity in adults was related to farm density during year 1, and to habitat fragmentation, farm density and altitude during year 2. The exposure of wild boar to T. gondii is thus variable according to landscape characteristics and probably results in a variable risk of transmission of toxoplasmosis to humans.

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