scholarly journals Do B chromosomes affect morphometric characters in yellow-necked mice Apodemus flavicollis (Rodentia, Mammalia)?

2000 ◽  
Vol 45 ◽  
pp. 129-135 ◽  
Author(s):  
Jelena Blagojević ◽  
Mladen Vujošević
Keyword(s):  
B Chromosomes ◽  
Apodemus Flavicollis ◽  
Morphometric Characters

scholarly journals New Data on Organization and Spatial Localization of B-Chromosomes in Cell Nuclei of the Yellow-Necked Mouse Apodemus flavicollis

Cells ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1819
Author(s):  
Tatyana Karamysheva ◽  
Svetlana Romanenko ◽  
Alexey Makunin ◽  
Marija Rajičić ◽  
Alexey Bogdanov ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Sex Chromosomes ◽  
Interphase Nucleus ◽  
Spatial Organization ◽  
Three Dimensional ◽  
Dna Probes ◽  
B Chromosomes ◽  
Apodemus Flavicollis ◽  
Yellow Necked Mouse

The gene composition, function and evolution of B-chromosomes (Bs) have been actively discussed in recent years. However, the additional genomic elements are still enigmatic. One of Bs mysteries is their spatial organization in the interphase nucleus. It is known that heterochromatic compartments are not randomly localized in a nucleus. The purpose of this work was to study the organization and three-dimensional spatial arrangement of Bs in the interphase nucleus. Using microdissection of Bs and autosome centromeric heterochromatic regions of the yellow-necked mouse (Apodemus flavicollis) we obtained DNA probes for further two-dimensional (2D)- and three-dimensional (3D)- fluorescence in situ hybridization (FISH) studies. Simultaneous in situ hybridization of obtained here B-specific DNA probes and autosomal C-positive pericentromeric region-specific probes further corroborated the previously stated hypothesis about the pseudoautosomal origin of the additional chromosomes of this species. Analysis of the spatial organization of the Bs demonstrated the peripheral location of B-specific chromatin within the interphase nucleus and feasible contact with the nuclear envelope (similarly to pericentromeric regions of autosomes and sex chromosomes). It is assumed that such interaction is essential for the regulation of nuclear architecture. It also points out that Bs may follow the same mechanism as sex chromosomes to avoid a meiotic checkpoint.

scholarly journals Exploring Supernumeraries - A New Marker for Screening of B-Chromosomes Presence in the Yellow Necked Mouse Apodemus flavicollis

PLoS ONE ◽  
2016 ◽  
Vol 11 (8) ◽  
pp. e0160946 ◽  
Author(s):  
Vanja Bugarski-Stanojević ◽  
Gorana Stamenković ◽  
Jelena Blagojević ◽  
Thomas Liehr ◽  
Nadezda Kosyakova ◽  
...  
Keyword(s):  
B Chromosomes ◽  
Apodemus Flavicollis ◽  
Yellow Necked Mouse

Differential gene expression in yellow-necked mice Apodemus flavicollis (Rodentia, Mammalia) with and without B chromosomes

Chromosoma ◽  
2005 ◽  
Vol 113 (8) ◽  
pp. 418-427 ◽  
Author(s):  
Nikola Tanić ◽  
Mladen Vujošević ◽  
Nasta Dedović-Tanić ◽  
Bogomir Dimitrijević
Keyword(s):  
Gene Expression ◽  
Differential Gene Expression ◽  
B Chromosomes ◽  
Apodemus Flavicollis ◽  
Differential Gene

scholarly journals Do B chromosomes affect fecundity in yellow-necked mice Apodemus flavicollis (Rodentia, Mammalia)?

2006 ◽  
Vol 58 (4) ◽  
pp. 221-223 ◽  
Author(s):  
Jelena Blagojevic ◽  
Vida Jojic ◽  
Vanja Bugarski-Stanojevic ◽  
Tanja Adnadjevic ◽  
M. Vujosevic
Keyword(s):  
B Chromosomes ◽  
Apodemus Flavicollis ◽  
Significant Difference ◽  
The Mean ◽  
Yellow Necked Mouse

The effects of the presence of B chromosomes on fecundity of the yellow-necked mouse Apodemus flavicollis were studied in 46 females without and 28 with Bs from four localities in Serbia. Uterine inspection showed that there was no significant difference in the mean number of scars and embryos between females with and those without Bs. Thus, B chromosomes do not appear to affect the fecundity of females carrying them, indicating that the presence of Bs does not affect fitness characteristics.

scholarly journals Screening of B chromosomes for presence of two genes in yellow-necked mice, Apodemus flavicollis (Mammalia, Rodentia)

Genetika ◽  
2015 ◽  
Vol 47 (1) ◽  
pp. 311-321 ◽  
Author(s):  
Marija Rajicic ◽  
Tanja Adnadjevic ◽  
Gorana Stamenkovic ◽  
Jelena Blagojevic ◽  
Mladen Vujosevic
Keyword(s):  
18S Rrna ◽  
Target Genes ◽  
Primordial Germ Cells ◽  
18S Rrna Gene ◽  
B Chromosomes ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Apodemus Flavicollis ◽  
Protein Coding ◽  
Exon 1

B chromosomes (Bs) are a very heterogeneous group of extra chromosomes. In various species Bs occur with different nucleotide sequences ranging from repetitive to protein coding. In yellow-necked field mice, Apodemus flavicollis Bs are small euchromatic chromosomes and untill now, only few molecular analyses have been conducted. In this study we examined A. flavicollis individuals with different number of Bs for presence of two genes, C-KIT and 18S rRNA. The C-KIT proto-oncogene was found on Bs in three Canidae species and one Cervidae species. This gene is a coding receptor critical for proliferation and cell differentiation of hematopoietic, melanoblast and primordial germ cells, and is highly conserved within mammals. While using semiquantitative PCR, we did not notice any difference in the C-KIT band intensity among animals with different number of Bs (0-3). The presence of only one copy of C- KIT gene was confirmed using real time-PCR on genomic DNA of A. flavicollis specimens with different number of Bs. rRNA genes in eukaryotes? genome are organized like units of tandem repeated sequences. The units form distinct clusters on one to several chromosome pairs. rRNA genes were found on Bs in different species including two species of genus Apodemus. One particular sample with 2 Bs showed the number of 18S rRNA gene about three times that of the calibrator 0 B sample. This result can indicate the presence of 18S rRNA gene on Bs, but its confirmation requires the implementation of other methods. Still, we can neither confirm nor deny the existence of pseudogen of tested target genes, or lose of exon 1 of C-KIT protooncogen in Bs of A. flavicollis. Our findings are further discussed.

The role of B chromosomes in the population dynamics of yellow-necked wood mice Apodemus flavicollis (Rodentia, Mammalia)

Genome ◽  
1995 ◽  
Vol 38 (3) ◽  
pp. 472-478 ◽  
Author(s):  
J. Blagojević ◽  
M. Vujošević
Keyword(s):  
Population Dynamics ◽  
Seasonal Changes ◽  
Population Decline ◽  
B Chromosomes ◽  
Stress Conditions ◽  
Apodemus Flavicollis ◽  
Wood Mice

Seasonal changes in the frequency of animals with B chromosomes were studied during 1989 in the population of yellow-necked wood mice, Apodemus flavicollis, on the mountain Jastrebac in Yugoslavia. During the study the estimated density of animals reached a very large value (378.8 individuals/ha for the April sample), which produced stress conditions leading to population decline. Six samples with a total of 247 specimens were karyotypically analyzed. Animals with Bs were present in all samples in frequencies that differed significantly at the 5% level. This difference was produced by the reduction in frequencies of specimens with Bs between the April and May samples (0.53 versus 0.29; [Formula: see text]; p < 0.002) and appeared mostly as a result of increased elimination of B-carrying animals from the prereproductive group. Young animals with Bs were inferior in stress conditions produced by overabundance of animals. From the first to the last sample, the frequency of animals with one B among B-containing animals constantly decreased, while the frequency of animals with more than one B increased. The possible role of Bs in regulating population dynamics is discussed.Key words: B chromosomes, Apodemus flavicollis, population dynamics, stress conditions.

scholarly journals B chromosomes and cranial variability in yellow-necked field mice (Apodemus flavicollis)

2011 ◽  
Vol 92 (2) ◽  
pp. 396-406 ◽  
Author(s):  
Vida Jojić ◽  
Jelena Blagojević ◽  
Mladen Vujošević
Keyword(s):  
B Chromosomes ◽  
Apodemus Flavicollis ◽  
Field Mice
Sign in / Sign up

Export Citation Format

Share Document