scholarly journals Studies on some paraclinical indices on intoxication in horses from freshly cut Jimson weed (Datura stramonium)-contaminated maize intended for ensiling : clinical communication

Author(s):  
R. Binev ◽  
I. Valchev ◽  
J. Nikolov

Monitoring of changes in some blood laboratory parameters in 34 horses after ingesting freshly harvested maize that was to be used for ensiling, heavily contaminated with young Datura stramonium plants, is described. For a 7-day period the following parameters were monitored: haemoglobin content (HGB), red blood cell counts (RBC), white blood cell counts (WBC), haematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), differential white cell counts (DWC), erythrocyte sedimentation rate (ESR), protein fractions, aspartate aminotransferase (AST), lactate dehydrogenase (LDH), total bilirubin (TB), direct bilirubin (DB), blood glucose (Glu), total protein (TP), globulin (Glob) and albumin (Alb). The intoxication was accompanied by erythrocytosis, leukocytosis, regenerative left shift neutrophilia, lymphopaenia, eosinopaenia, increased haematocrit values, low erythrocyte sedimentation rate, hyperglycaemia, bilirubinaemia, hypoproteinaemia and increased activity of AST and LDH. No changes occurred in the mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), albumin, globulin and globulin fractions (a1, a2, b1, b2 and g). The blood parameters returned to normal between post-intoxication days 2 and 5. The observed changes in clinical chemistry indices could be used in the diagnosis, differential diagnosis and prognosis of Jimson weed intoxication.

Perfusion ◽  
1991 ◽  
Vol 6 (1) ◽  
pp. 41-50 ◽  
Author(s):  
SK Naik ◽  
A. Knight ◽  
MJ Elliott

Total body water (TBW) is increased after cardiopulmonary bypass (CPB) resulting in tissue oedema and organ dysfunction. Ultrafiltration has been used to reduce this accumulation of water, although conventional ultrafiltration seemed ineffective in reducing the rise in TBW after CPB in our clinical experience. We describe a modified technique in which ultrafiltration is performed in the first 10 minutes after the patient is weaned from bypass, returning nearly all the blood in the circuit to the patient and elevating the haematocrit (Hct) to any predetermined level. We carried out a pilot study on 21 children aged 4-144 months undergoing open-heart surgery and CPB for congenital heart defects. They were divided into three comparable groups: (1) controls (n = 6); (2) conventional ultrafiltration (n = 7); and (3) modified ultrafiltration (n = 8). TBW (bio-impedance), Hct, osmolality, mean corpuscular volume and mean corpuscular haemoglobin concentration were recorded at frequent intervals. Control patients showed elevation of TBW by 18.2% median (range 14.5-20.3), conventional ultrafiltration by 12.4% (7.9-15.0), modified ultrafiltration by 5.7% (4.5-7.1) (p < 0.0001 compared to controls, p < 0.005 compared to conventional ultrafiltration, Mann-Whitney U test). Hct could be elevated to preoperative levels only by the modified method. Mean corpuscular volume, and mean corpuscular haemoglobin concentration osmolality were unaltered. Ultrafiltration by the modified method was more effective than conventional ultrafiltration in reducing the rise in TBW and elevating Hct after CPB.


2010 ◽  
Vol 79 (9) ◽  
pp. S3-S8 ◽  
Author(s):  
Eva Straková ◽  
Pavel Suchý ◽  
Romana Kábelová ◽  
František Vitula ◽  
Ivan Herzig

The present paper extends the knowledge of selected blood indicators in six species of feathered game such as helmeted guineafowl (Numida meleagris), common pheasant (Phasianus colchicus), Japanese quail (Coturnix coturnix japonica), chukar (Alectoris chucar), grey partridge (Perdix perdix) and wild turkey (Meleagris gallopavo), reared in the Czech Republic and Europe. The red blood cell (RBC) values in individual species of feathered game ranged as follows: the mean RBC counts varied in a range of 2.10 - 2.58 T/l; haematocrit values were in a range of 0.33 - 0.39 1/1; haemoglobin concentration varied in a range of 101.20 - 129.13 g/l; mean corpuscular haemoglobin concentration (MCHC) ranged from 28.63 to 35.44%; mean corpuscular haemoglobin (MCH) varied in a range of 46.15 - 62.07 pg and the mean corpuscular volume (MCV) was 147.66 - 178.20 fl. Except for the total concentration of haemoglobin and the mean corpuscular haemoglobin concentration, no significant differences were found between the mean values of RBC values in individual species of feathered game. However, some differences between species were found in white blood cell (WBC) values, ranging as follows: the total WBC count ranged from 13.55 to 26.90 G/l, basophil granulocytes from 0.011 to 0.110 G/l, eosinophil granulocytes from 0.011 to 0.593 G/l, neutrophil granulocytes from 1.868 to 6.661 G/l, lymphocytes from 10.937 to 20.922 G/l and monocytes from 0.034 to 0.199 G/l. Most values showed significant (P ⪬ 0.05) to highly significant (P ⪬ 0.01) interspecies differences. The main goal of the study was to investigate selected haematological indicators in six major species of feathered game.


1967 ◽  
Vol 18 (3) ◽  
pp. 523 ◽  
Author(s):  
C Dobson

Variations in the numbers of erythrocytes and white cells within the blood of sheep given various doses of Oesophagostomum columbianum larvae were studied over a period of 10 weeks. The sheep showed losses of red cells and haemoglobin, and the packed cell volume was diminished after infestation with O. columbianum. These effects increased in severity as the infesting dose was increased; the resistant state of the host also affected the blood picture. In all infestations the calculated absolute values for the blood, i.e. mean corpuscular haemoglobin (MCH), mean corpuscular volume (MCV), and mean corpuscular haemoglobin concentration (MCHC), did not significantly change except in the heavier infestations, where MCV increased for a time. The type of anaemia which predominated throughout these experiments was normocytic-normochromic. Where the MCV increased for a period, the anaemia was of the macrocytic-normochromic type. During the first infestation with 2000 larvae the anaemias tended to become microcytiohyperchromic, but these changes were not significant. Gross observations on the gut indicated that one of the factors influencing the development of anaemias was the extensive tissue damage caused by the larvae, which resulted in chronic bleeding from the mucosa. This suggestion was supported by the fact that anaemias also developed during second infestations when there was no adult worm population. The white cell counts from a number of these sheep were also recorded. The most significant changes occurred after second infestation, although a general increase was noted after all first infestations as well; prominent increases occurred in the agranulocytes and the eosinophil leucocytes. The neutrophil leucocyte counts fell throughout all the infestation. All the normal values obtained for the haemoglobin concentration, packed cell volume, red cell counts, MCV, MCH, and MCHC and white cell counts compared favourably with the values cited by other workers.


2017 ◽  
Vol 62 (No. 7) ◽  
pp. 401-405 ◽  
Author(s):  
F. Fazio ◽  
V. Ferrantelli ◽  
C. Saoca ◽  
G. Giangrosso ◽  
G. Piccione

The aim of this study was to investigate the influence of storage time at +4 °C on haematological indicators in rainbow trout Oncorhynchus mykiss (Walbaum, 1792). For this purpose, 60 Italian trout were evaluated and red blood cells, white blood cells, thrombocyte count, haematocrit, haemoglobin concentration, mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration were determined. After blood collection (T<sub>0</sub>), all samples were immediately analysed using an automatic method to assess the haematological parameters and then divided into two different aliquots and stored at +4 °C. The first aliquot was refrigerated for 168 h (T<sub>1</sub>), the second one for 336 h (T<sub>2</sub>). Statistical analysis (one-way repeated-measures ANOVA) showed a significant effect of storage time (P &lt; 0.05) on red blood cells, thrombocyte count, haematocrit, haemoglobin concentration, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration. The obtained data expand our knowledge of haematological evaluation techniques and could contribute to the development of an appropriate method for haematological analysis that is suitable for the evaluation of the health status of wild and farmed fish.


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