Fe catalysed alkene oxidation: Effect of steric and electronic properties on non-heme oxygenase activity

The synthetic metalloporphyrin, Cr-protoporphyrin, as a potent competitive inhibitor of heme oxygenase activity in rat spleen, liver, and kidney. When administered to neonatal animals in a single dose immediately after birth, Cr-protoporphyrin suppresses postnatal hyperbilirubinemia and produces a marked and sustained lowering of heme oxidation activity in liver, spleen, and kidney. The metalloporphyrin also potently inhibited the rate of heme degradation to bile pigment in human spleen.

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Heme oxygenase activity causes transient hypersensitivity to oxidative ultraviolet a radiation that depends on release of iron from heme

Free Radical Biology and Medicine ◽

10.1016/s0891-5849(00)00205-7 ◽

2000 ◽

Vol 28 (8) ◽

pp. 1191-1196 ◽

Cited By ~ 33

Author(s):

Egil Kvam ◽

Vidya Hejmadi ◽

Stefan Ryter ◽

Charareh Pourzand ◽

Rex M Tyrrell

Keyword(s):

Heme Oxygenase ◽

Ultraviolet A ◽

Heme Oxygenase Activity ◽

Oxygenase Activity

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Heme oxygenase activity and hemoglobin neurotoxicity are attenuated by inhibitors of the MEK/ERK pathway

Neuropharmacology ◽

10.1016/j.neuropharm.2009.01.022 ◽

2009 ◽

Vol 56 (5) ◽

pp. 922-928 ◽

Cited By ~ 19

Author(s):

Jing Chen-Roetling ◽

Zhi Li ◽

Mai Chen ◽

Olatilewa O. Awe ◽

Raymond F. Regan

Keyword(s):

Heme Oxygenase ◽

Erk Pathway ◽

Heme Oxygenase Activity ◽

Oxygenase Activity

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281 HEME OXYGENASE ACTIVITY IN THE MOUSE PLACENTA DURING FETAL DEVELOPMENT.:

Journal of Investigative Medicine ◽

10.2310/6650.2005.x0004.280 ◽

2006 ◽

Vol 54 (1) ◽

pp. S128.3-S128

Author(s):

H. Zhao ◽

R. J. Wong ◽

I. Morioka ◽

F. A. Kalish ◽

D. K. Stevenson

Keyword(s):

Heme Oxygenase ◽

Fetal Development ◽

Heme Oxygenase Activity ◽

Mouse Placenta ◽

Oxygenase Activity

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Modified endogenous carbon monoxide production through modulation of heme oxygenase activity alters some aspects of the cold restraint stress response in male albino rats

Endocrine Regulations ◽

10.4149/endo_2012_04_205 ◽

2012 ◽

Vol 46 (04) ◽

pp. 205-215 ◽

Cited By ~ 2

Author(s):

S. El-Sayed ◽

M. Hassan ◽

M. Ibrahim ◽

E. Elbassuoni ◽

N. Aziz

Keyword(s):

Carbon Monoxide ◽

Stress Response ◽

Heme Oxygenase ◽

Restraint Stress ◽

Albino Rats ◽

Heme Oxygenase Activity ◽

Oxygenase Activity ◽

Cold Restraint Stress ◽

Cold Restraint

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Gas-chromatographic assay for heme oxygenase activity.

Clinical Chemistry ◽

10.1093/clinchem/28.10.2026 ◽

1982 ◽

Vol 28 (10) ◽

pp. 2026-2032 ◽

Cited By ~ 23

Author(s):

F W Sunderman ◽

J R Downs ◽

M C Reid ◽

L M Bibeau

Keyword(s):

Gas Chromatography ◽

Detection Limit ◽

Heme Oxygenase ◽

Liver Microsomes ◽

Microsomal Protein ◽

Spectrophotometric Assay ◽

Rat Tissues ◽

Heme Oxygenase Activity ◽

Oxygenase Activity ◽

Kidney Microsomes

Abstract We have developed an improved assay for microsomal heme oxygenase activity, based on the enzymic release of CO from the alpha-methene bridge of hemin and the quantitation of CO by gas chromatography. The within-run coefficient of variation (CV) of heme oxygenase assays in microsomes from rat tissues (liver, kidney) averaged 8%; the between-run CV averaged 15%. The detection limit for heme oxygenase activity was approximately 1 nmol/h per milligram of microsomal protein. Gas-chromatographic assays of heme oxygenase activities in rat tissues correlated well (r = 0.94) with results by a spectrophotometric assay based on bilirubin production. In untreated rats, heme oxygenase activity averaged 7 +/- 3 nmol/h per milligram of protein (n = 36) in kidney microsomes and 14 +/- 5 nmol/h per milligram of protein (n = 17) in liver microsomes. Heme oxygenase activity was increased 10-fold in kidney microsomes and threefold in liver microsomes from rats killed 17 h after subcutaneous injection of NiCl2 (0.5 mmol/kg body wt). These findings illustrate the efficacy of the gas-chromatographic assay for measuring xenobiotic effects on heme oxygenase activity.

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