scholarly journals Evaluation of lymphangiogenesis in acellular dermal matrix

2014 ◽  
Vol 47 (03) ◽  
pp. 318-324 ◽  
Author(s):  
Mario Cherubino ◽  
Igor Pellegatta ◽  
Federico Tamborini ◽  
Michele Cerati ◽  
Fausto Sessa ◽  
...  
Keyword(s):  
Wound Healing ◽  
Endothelial Cells ◽  
Cell Invasion ◽  
Acellular Dermal Matrix ◽  
Wound Contraction ◽  
Control Group ◽  
Dermal Substitute ◽  
Dermal Matrix ◽  
Microscopic Evaluation ◽  
Acellular Dermal

ABSTRACT Introduction: Much attention has been directed towards understanding the phenomena of angiogenesis and lymphangiogenesis in wound healing. Thanks to the manifold dermal substitute available nowadays, wound treatment has improved greatly. Many studies have been published about angiogenesis and cell invasion in INTEGRA®. On the other hand, the development of the lymphatic network in acellular dermal matrix (ADM) is a more obscure matter. In this article, we aim to characterize the different phases of host cell invasion in ADM. Special attention was given to lymphangiogenic aspects. Materials and Methods: Among 57 rats selected to analyse the role of ADM in lymphangiogenesis, we created four groups. We performed an excision procedure on both thighs of these rats: On the left one we did not perform any action except repairing the borders of the wound; while on the right one we used INTEGRA® implant. The excision biopsy was performed at four different times: First group after 7 days, second after 14 days, third after 21 days and fourth after 28 days. For our microscopic evaluation, we used the classical staining technique of haematoxylin and eosin and a semi-quantitative method in order to evaluate cellularity counts. To assess angiogenesis and lymphangiogenesis development we employed PROX-1 Ab and CD31/PECAM for immunohistochemical analysis. Results: We found remarkable wound contraction in defects that healed by secondary intention while minor wound contraction was observed in defects treated with ADM. At day 7, optical microscopy revealed a more plentiful cellularity in the granulation tissue compared with the dermal regeneration matrix. The immunohistochemical process highlighted vascular and lymphatic cells in both groups. After 14 days a high grade of fibrosis was noticeable in the non-treated group. At day 21, both lymphatic and vascular endothelial cells were better developed in the group with a dermal matrix application. At day 28, lymphatic endothelial cells had organized themselves, engineering the pseudocylindrical structure better disposed in the ADM group than in the control group, and the lymphatic cells were detectable inside the vessels’ lumen in this group. Conclusion: This study has made it possible to demonstrate the absolute importance of an ADM in proper wound healing and has shown better definition of both the qualitative and quantitative aspects of lymphangiogenesis compared to the second intention healing. A major grade of organization of the extracellular matrix and a minor grade of fibrosclerosis in ADM allowed a well-structured morphologic and functional development of the endothelial and lymphatic vascular structures. This study hopes to represent a clinical basis for a wider use of ADM in lesions where lymphatic complications are common.

scholarly journals The Utility of Novel Fish-Skin Derived Acellular Dermal Matrix (Kerecis) as a Wound Dressing Material

2021 ◽  
Vol 17 (1) ◽  
pp. 39-47
Author(s):  
Tae Hyung Kim ◽  
Jun Ho Park ◽  
Hyun Gyo Jeong ◽  
Syeo Young Wee
Keyword(s):  
Wound Healing ◽  
Healing Rate ◽  
Computer Software ◽  
Acellular Dermal Matrix ◽  
Control Group ◽  
Omega 3 ◽  
Dermal Matrix ◽  
Burn Wounds ◽  
Novel Material ◽  
Acellular Dermal

Background: The newly-approved Kerecis is a piscine acellular dermal xenograft. This piscine acellular dermal matrix (ADM) has specific bioactive lipid mediators, omega-3 polyunsaturated fatty acids, and has a positive effect on the process of wound healing. This study aimed to explore the utility of this novel material by comparing healing rates, and suggest the proper timing for applying Kerecis.<br/>Methods: Patients who visited the hospital with acute or chronic deep dermal wounds from June 2019 to May 2020 were enrolled in the study. A total of 48 patients were assessed. All wounds in the experimental group (n=16) were treated only once with Kerecis and a non-adherent absorptive foam material (Therasorb) to cover the ADM. In the control group, daily conventional dressings were provided. All wounds sizes were measured with mass-market computer software in a method suggested by the authors for the first time.<br/>Results: The mean healing rate proved to be faster in the Kerecis group (P<0.05) versus the control group, and no complications were observed. It was statistically proved that treating burn wounds with the ADM showed better healing rates than the conventional method (P<0.05).<br/>Conclusion: This study establishes that managing wounds with the ADM is likely to heal wounds faster than traditional dressings. In addition, for burn wounds, a prolonged application (10 days vs. 5 days after the onset) showed a better wound healing rate (98.8%±2.5% vs. 67.0%±14.3%, respectively, P=0.029).

scholarly journals Effects of allogeneic mouse adipose-derived mesenchymal stem cell-microporous sheep acellular dermal matrix on healing of wound with full-thickness skin defect in mouse and the related mechanism

2019 ◽  
Vol 6 (2) ◽  
pp. 26
Author(s):  
Shengjun C ◽  
Lingfeng W ◽  
Te B ◽  
Xue F ◽  
Fang L ◽  
...  
Keyword(s):  
Wound Healing ◽  
Granulation Tissue ◽  
Acellular Dermal Matrix ◽  
Control Group ◽  
Skin Defect ◽  
Full Thickness ◽  
Dermal Matrix ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Acellular Dermal

Objective: To explore the effects of allogeneic mouse adipose-derived mesenchymal stem cell (ADSC)-microporous sheep acellular dermal matrix (ADM) on wound healing of full-thickness skin defect in mice and the related mechanism.Methods: One Kunming mouse was sacrificed by cervical dislocation to collect adipose tissue from the inguinal region. Mouse ADSCs were isolated from the adipose tissue and cultured in vitro. Cells in the third passage were identified by cell adipogenic and osteogenic differentiation. The expressions of CD34, CD73, CD90, and CD105 were analyzed by flow cytometer. After one sheep was sacrificed with the skin of its back cut off, microporous sheep ADM was prepared by using acellular processing and freeze-thaw method. A round and full-thickness skin defect wound, with a diameter of 12 mm, was made on the back of each of 36 Kunming mice. The wounds were covered by microporous sheep ADM. The mice were divided into ADSC group and control group with 18 mice in each group according to the random number table method after surgery. A volume of 0.2 ml of DMEM/F12 culture medium containing 1 × 106 ADSCs was injected between microporous sheep ADM and the wound of each mouse in ADSC group, while 0.2 ml of DMEM/F12 culture medium was injected between microporous sheep ADM and the wound of each mouse in control group. At post-surgery day (PSD) 12 and 17, the wound healing rate in each group was calculated respectively; wound vascularization in 2 groups of mice was observed under the reverse irradiation of back light; and the granulation tissue in the wound in ADSC group was observed by means of hematoxylin-eosin staining. At PSD 7, the thickness of the granulation tissue in the wound was measured in each group of mice. At PSD 12 and 17, the immunohistochemical method was used to detect the expression of VEGF in each group of mice. The number of samples was 6 in each group at each time point in the above experiments. The data obtained were processed with t-test and factorial design ANOVA.Results: (1) After 7 days of adipogenic induction, red lipid droplets were observed in the cytoplasm with oil red O staining. After 21 days of osteogenic induction, black calcium deposition was observed in the medium stained with silver nitrate. The expression levels of CD73, CD90, CD 105 and CD34 in cells were 97.82%, 99.32%, 97.35% and 5.88% respectively. The cells were identified as ADSCs. (2) The wound healing rates of ADSC group at PSD 12 and 17 [(78 ± 6)%, (98 ± 3)%] were significantly higher than those of control group at PSD 12 and 17 [(60 ± 9)%, (90 ± 4)%, t = 4.26, 4.46, p< .01]. (3) At PSD 7, no vessels obviously grew into the center of the wound in both groups of mice, while the granulation tissue already covered the wound in ADSC group. At PSD 12, the wound in ADSC group was more well-perfused than control group. At PSD 17, it was observed that large vessels were crossing through the whole wound in ADSC group, while large vessels were observed without crossing through the whole wound in control group. (4) In ADSC group, at PSD 7, the wound was covered with thin granulation tissue, and the granulation tissue was obviously thickened at PSD 12. At PSD 17, the granulation tissue was covered by epidermis. At PSD 7, the thickness of the granulation tissue in the wound in ADSC group [(0.62 ± 0.05) mm] was significantly greater than that in control group [(0.31 ± 0.04) mm, t = 12.27, p < .01]. (5) At PSD 12 and 17, the expression levels of VEGF in the wound in ADSC group [(80.7 ± 2.2), (102.8 ± 2.6)/mm2] were significantly than those in control group [(59.5 ± 2.4), (81.5 ± 2.6)/mm2, t = 15.95, 14.14, p < .01].Conclusions: Allogeneic mouse ADSC-microporous sheep ADM can promote angiogenesis and the growth of granulation tissue in the wound with full-thickness skin defect in mice, thus accelerating wound healing. The mechanism is probably related with the increase in the expression of VEGF.

Application of paste-type acellular dermal matrix in hard-to-heal wounds

2021 ◽  
Vol 30 (5) ◽  
pp. 414-418
Author(s):  
Sang Wha Kim ◽  
Hyung Sup Shim ◽  
Jihye Lee ◽  
Youn Hwan Kim
Keyword(s):  
Wound Healing ◽  
Adverse Events ◽  
Acellular Dermal Matrix ◽  
Complete Healing ◽  
Dermal Matrix ◽  
Wound Area ◽  
Number Of Patients ◽  
Complete Wound Healing ◽  
Zero Baseline ◽  
Acellular Dermal

Objective: The extracellular matrix (ECM) is one of the most important elements in wound healing. Absence or dysfunction of the ECM may impair wound healing. The application of acellular dermal matrix (ADM) as a substitute for ECM has been suggested. This study investigated the clinical application and wound healing effects of a paste-type ADM in patients presenting with hard-to-heal wounds due to various causes. Method: Patients with a hard-to-heal wound for >1 month, from September 2017 to February 2019, were included in this study. After debridement, the paste-type ADM was applied, at zero (baseline), two and four weeks. After application of the paste-type ADM, a conventional dressing was applied using polyurethane foam. Wound size, the formation of granulation tissue, re-epithelialisation, complete healing and adverse events were recorded at zero (baseline), one, two, four, eight and 12 weeks after the initial treatment. Results: A total of 18 patients took part (eight male, 10 female, mean age of 56±16.16 years). The mean wound area decreased from 17.42±10.04cm2 to 12.73±7.60cm2 by week one (p<0.05), to 10.16±7.00 by week two (p<0.0005), to 5.56±5.25 by week four (p<0.0001), to 2.77±5.15 by week eight (p<0.0001) and to 2.07±4.78 by week 12 (p<0.0001). The number of patients with >75% re-epithelialisation increased from two (11.1%) at two weeks to five (27.8%) at four weeks, to 11 (61.1%) at eight weeks and to 13 (72.2%) at 12 weeks. The number of patients showing complete wound healing was two (11.1%) at four weeks, nine (50.0%) at eight weeks and 12 (66.7%) at 12 weeks. No adverse events were reported during treatment. Conclusion: The paste-type ADM used in this study is a viable option for facilitating wound healing; it can shorten hospitalisation, and promote a faster recovery and return to normal life activities.

Xenogeneic Acellular Dermal Matrix as a Dermal Substitute in Rats

1999 ◽  
Vol 20 (5) ◽  
pp. 382-390 ◽  
Author(s):  
Anil Srivastava ◽  
Lawrence J. Jennings ◽  
Marella Hanumadass ◽  
Stephen Sethi ◽  
Evangeline DeSagun ◽  
...  
Keyword(s):  
Acellular Dermal Matrix ◽  
Dermal Substitute ◽  
Dermal Matrix ◽  
Acellular Dermal

scholarly journals MSCs on an acellular dermal matrix (ADM) sourced from neonatal mouse skin regulate collagen reconstruction of granulation tissue during adult cutaneous wound healing

RSC Advances ◽  
2017 ◽  
Vol 7 (37) ◽  
pp. 22998-23010 ◽  
Author(s):  
Maosheng Chen ◽  
Ying Jin ◽  
Xue Han ◽  
Ning Wang ◽  
Xiaoyuan Deng ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mouse Skin ◽  
Acellular Dermal Matrix ◽  
Neonatal Mouse ◽  
The Novel ◽  
Cutaneous Wound Healing ◽  
Dermal Matrix ◽  
Cutaneous Wound ◽  
Novel Strategy ◽  
Acellular Dermal

The novel strategy of MSCs seeded on ADM sourced from neonatal mouse skin promotes full-thickness cutaneous wound healing.

scholarly journals Use of Porcine Acellular Dermal Matrix as a Dermal Substitute in Rats

2001 ◽  
Vol 233 (3) ◽  
pp. 400-408 ◽  
Author(s):  
Anil Srivastava ◽  
Evangeline Z. DeSagun ◽  
Lawrence J. Jennings ◽  
Stephen Sethi ◽  
Anan Phuangsab ◽  
...  
Keyword(s):  
Acellular Dermal Matrix ◽  
Dermal Substitute ◽  
Dermal Matrix ◽  
Porcine Acellular Dermal Matrix ◽  
Acellular Dermal

scholarly journals A barrier against reactive oxygen species: chitosan/acellular dermal matrix scaffold enhances stem cell retention and improves cutaneous wound healing

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Wei Lin ◽  
Xiaoyang Qi ◽  
Wenjing Guo ◽  
Danyang Liang ◽  
Heting Chen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Delivery System ◽  
Acellular Dermal Matrix ◽  
Cell Delivery ◽  
Stem Cell Therapies ◽  
Dermal Matrix ◽  
Cell Therapies ◽  
Stem Cell Delivery ◽  
Acellular Dermal

Abstract Background Stem cell therapies have gained great attention for providing novel solutions for treatment of various injuries and diseases due to stem cells’ self-renewal, ability to differentiate into various cell types, and favorite paracrine function. Nevertheless, the low retention of transplanted stem cell still limits their clinical applications such as in wound healing in view of an induced harsh microenvironment rich in reactive oxygen species (ROS) during inflammatory reactions. Methods Herein, a novel chitosan/acellular dermal matrix (CHS/ADM) stem cell delivery system is developed, which is of great ROS scavenging activity and significantly attenuates inflammatory response. Result Under ROS microenvironment, this stem cell delivery system acts as a barrier, effectively scavenging an amount of ROS and protecting mesenchymal stem cells (MSCs) from the oxidative stress. It notably regulates intracellular ROS level in MSCs and reduces ROS-induced cellular death. Most importantly, such MSCs delivery system significantly enhances in vivo transplanted stem cell retention, promotes the vessel growth, and accelerates wound healing. Conclusions This novel delivery system, which overcomes the limitations of conventional plain collagen-based delivery system in lacking of ROS-environmental responsive mechanisms, demonstrates a great potential use in stem cell therapies in wound healing.

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