scholarly journals Dentin comparison in primary and permanent molars under transmitted and polarised light microscopy: An in vitro study

Author(s):  
N Chowdhary ◽  
SubbaV.V Reddy
2017 ◽  
Vol 5 (5) ◽  
pp. 664-666 ◽  
Author(s):  
Rajnish K. Singhal ◽  
Balwant Rai

BACKGROUND: Different formulations of dentifrices exist in the market. Usually, single toothpaste is used by all family members including children. There is a big concern of fluoride ingestion with the toothpaste containing high fluoride content in children. Recently, new toothpaste (including toothpaste) with remineralization potential without fluoride content has been formulated.AIM: There is an urgent need to compare remineralization potential of this new formulation with the exiting dentifrices. Therefore, the present study has been undertaken to assess and compare the remineralization potential of three dentifrices with different compositions on artificially induced carious lesions in vitro by using scanning electron microscopy and polarised light microscopy.MATERIALS AND METHODS: The present in vitro study was conducted on 21 healthy extracted primary central incisor teeth surfaces, which were divided into three groups and were treated by three different dentifrices. Artificial demineralization was followed by remineralization using dentifrice slurry as per the group distribution. All the samples were studied for remineralization by using scanning electron microscopy and polarised light microscopy. Data were analysed using SPSS version 11 software.RESULTS: A significant difference was found between the remineralization potential of  incudent toothpaste and other toothpaste groups based on the analysis of polarised light microscopy and stereomicroscope. The remineralizing ability of  incudent toothpaste for artificial enamel lesions was found to be significantly higher than that of Colgate® and Crest toothpaste.CONCLUSIONS: The limitations of this study include, being a short term study, low sample size and in vitro experiment.  incudent toothpaste has exhibited a higher remineralizing potential as compared to fluoride based toothpaste in our study.


Author(s):  
Meriem Fejjeri-Mezghanni

This in vitro study aimed to evaluate the micro-leakage at the occlusal and cervical margins in class II cavities in permanent molars restored with a low shrink silorane resin composite (Filtek P90) applied with or without etching and two methacrylate-based composite resins (valux plus and Amelogen® plus). Two standardised class II box cavities were prepared in proximal surfaces of 60 sound non-fluorotic permanent molars which were divided into three groups of 20 each according to the type of composite. Group I: silorane-based composite (Filtek P90) in the mesial cavity and methacrylate-based microhybrid composite (Adper single bond 2/valux plus) in the distal cavity, Group II: silorane-based composite in the mesial cavity and silorane-based composite with previous etching in the distal cavity, and Group III: silorane-based composite in the mesial cavity and methacrylate-based micro-hybrid composite (PQ1 /Amelogen plus) in the distal cavity. The samples underwent an artificial aging with thermocycling. Root apices were sealed with transparent orthodontic resin and coated with transparent varnish except 1 mm around the restoration. The specimens were immersed in 1% methylene blue solution at room temperature for 48 hour. The samples were sectioned longitudinally in the mesiodistal direction and evaluated under a stereomicroscope at 20X. Scoring was done according to the depth of methylene blue penetration into the cavities. Statistical analysis of the data was performed using SPSS 17.0 software. Silorane-based composite resin showed less micro-leakage than the methacrylate-based composites. Etching improved the sealing ability of silorane restorations. Silorane-based composite preceded by etching had less micro-leakage among the other materials used in this in vitro study. Keywords: Silorane, methacrylate based composite, sealing, micro-leakage, class II cavity


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