Clinico-microbiological profile of sepsis with carbapenem-resistant Gram-negative isolates among patients presenting to a large tertiary care hospital in South Kerala

The emergence of bacterial antibiotic resistance is a cardinal concern in the health care system. The spread of resistance in Enterobacteriaceae and non-fermenters to the currently available drugs make the treatment of serious nosocomial infections troublesome. The purpose of the study is to find out the carbapenem resistance among Gram-negative bacilli in a tertiary care hospital. Antibiotic susceptibility pattern of 1913 aerobic Gram-negative bacilli isolated from clinical samples was made for a period of 6 months. All the isolates were tested for susceptibility to antibiotics by the Kirby-Bauer disc diffusion technique according to CLSI guidelines. Carbapenemase production was confirmed by the Modified Hodge Test (MHT). Minimum Inhibitory Concentration (MIC) by Epsilometer (E) test was performed (for Imipenem and Meropenem) for carbapenem-resistant strains. A total of 1731 clinical samples, 1913 Gram-negative bacilli were isolated. 1476 (77.1%) were Enterobacteriaceae and 433 (22.6%) were non-fermenters. 54 were carbapenemase-producing Gram-negative bacilli. Meropenem E test was done for carbapenemase-producing Gram-negative bacilli. The minimum inhibitory concentration for Meropenem ranged from 0.002μg/ml to 32μg/ml. To overcome the problem of emerging resistance, combined interaction and cooperation of microbiologists, clinicians and the infection control team is needed.

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Prevalence of Carbapenem Resistant Gram Negative Bacilli Harboring blaNDM-1 Gene Isolated in a Tertiary Care Hospital

Current Research in Bacteriology ◽

10.3923/crb.2016.9.16 ◽

2015 ◽

Vol 9 (1) ◽

pp. 9-16

Author(s):

Manisa Sahu ◽

Priyadharshini Sekar ◽

Revathy Ramalingam ◽

Pallavi Bhalekar ◽

E. Suguna ◽

...

Keyword(s):

Tertiary Care Hospital ◽

Tertiary Care ◽

Care Hospital ◽

Gram Negative ◽

Carbapenem Resistant

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1397Characterization of Carbapenem Resistant Gram Negative Bacilli Isolated in a Tertiary Care Hospital: Epidemiology and Treatment Outcome

Open Forum Infectious Diseases ◽

10.1093/ofid/ofu052.943 ◽

2014 ◽

Vol 1 (suppl_1) ◽

pp. S368-S368

Author(s):

Sweta Shah ◽

Pooja Shah

Keyword(s):

Treatment Outcome ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Care Hospital ◽

Hospital Epidemiology ◽

Gram Negative ◽

Carbapenem Resistant

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Plethora of Resistance Genes in Carbapenem-Resistant Gram-Negative Bacteria in Greece: No End to a Continuous Genetic Evolution

Microorganisms ◽

10.3390/microorganisms10010159 ◽

2022 ◽

Vol 10 (1) ◽

pp. 159

Author(s):

Katerina Tsilipounidaki ◽

Zoi Athanasakopoulou ◽

Elke Müller ◽

Sindy Burgold-Voigt ◽

Zoi Florou ◽

...

Keyword(s):

Multidrug Resistance ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Routine Practice ◽

Gram Negative Bacteria ◽

Care Hospital ◽

Genetic Evolution ◽

Gram Negative ◽

Central Greece ◽

Carbapenem Resistant

Carbapenem-resistant Gram-negative bacteria are a public health threat that requires urgent action. The fact that these pathogens commonly also harbor resistance mechanisms for several other antimicrobial classes further reduces patient treatment options. The present study aimed to provide information regarding the multidrug resistance genetic background of carbapenem-resistant Gram-negative bacteria in Central Greece. Strains from a tertiary care hospital, collected during routine practice, were characterized using a DNA microarray-based assay. Various different resistance determinants for carbapenems, other beta-lactams, aminoglycosides, quinolones, trimethoprim, sulfonamides and macrolides were detected among isolates of the same sequence type. Eighteen different multidrug resistance genomic profiles were identified among the twenty-four K. pneumoniae ST258, seven different profiles among the eight K. pneumoniae ST11, four profiles among the six A. baumannii ST409 and two among the three K. oxytoca. This report describes the multidrug resistance genomic background of carbapenem-resistant Gram-negative bacteria from a tertiary care hospital in Central Greece, providing evidence of their continuous genetic evolution.

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