Autologous platelet-rich fibrin matrix in non-healing trophic ulcers in patients with Hansen's disease

Patients with Hansen's disease are liable to develop non-healing trophic ulcers. The aim of this study was to determine the efficacy of autologous platelet rich fibrin (PRF) applied at weekly intervals in the management of trophic ulcers. The mean age of the patients, duration and size of ulcer were 44.3 years, 7.4 months and 6.25cm2 respectively. After the third sessions of weekly dressing, there was a significant reduction in the ulcer area (p value = 0.015). All ulcers healed by a maximum of six weeks. No adverse events were noted. PRF thus seems a feasible, safe, simple and cost-effective treatment method.

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Obsidian ASG® Autologous Platelet-Rich Fibrin Matrix and Colorectal Anastomotic Healing: A Preliminary Study

Surgical Technology Online ◽

10.52198/21.sti.39.cr11508 ◽

2021 ◽

Vol 39 ◽

Author(s):

Andreas Shamiyeh ◽

◽

Bettina Klugsberger ◽

Carina Aigner ◽

Wolfgang Schimetta ◽

...

Keyword(s):

Anastomotic Leakage ◽

Anastomotic Leak ◽

Colorectal Resection ◽

Rectal Resection ◽

Leak Rate ◽

Anastomotic Healing ◽

Platelet Rich Fibrin ◽

Anastomotic Leak Rate ◽

Fibrin Matrix ◽

Autologous Platelet

Introduction: Anastomotic leakage (AL) following colorectal resection is a devastating complication affecting morbidity, mortality, and quality of life of patients in the long term. Different tissue sealants and biologic glues were tested showing conflicting results regarding their influence on anastomotic healing and leak prevention. Application of autologous platelet-rich fibrin (Vivostat A/S, Alleroed, Denmark), which acts as a source of angiogenic growth factors and cytokines, showed promising results in an in-vivo porcine model. Herein, we present the first human study of stapled colorectal anastomoses supplemented with an autologous-derived platelet-rich fibrin matrix (Obsidian ASG®, Rivolution GmbH, Rosenheim, Germany and Vivostat A/S, Alleroed, Denmark). Materials and Methods: A retrospective analysis of prospectively accumulated data was performed in two colorectal centers (Linz, Vienna) on patients undergoing left-sided colorectal or coloanal stapled anastomosis between October 2018 and December 2019. The Obsidian ASG® Matrix was applied to the rectal stump, and after closure with the circular stapling device, at the circumference of anastomosis in every single case. Anastomoses were supplemented with intra- and extra-anastomotic application (IAA—intra-anastomotic application developed by Rivolution GmbH, Rosenheim, Germany) of Obsidian ASG® Matrix. The primary endpoints were incidence of perioperative complications and anastomotic leak rate. Results: Two-hundred-sixty-one (138 female) patients underwent left-sided colonic (n=177) or rectal resection (n=84). In 253 (96.9%) cases, a laparoscopic or robotic-assisted approach was used. There were no complications attributable to the intraoperative application of the Obsidian ASG® Matrix. All intraoperative leak tests were negative. Overall, anastomotic leak rate accounted for 2.3% (6/261). AL following colonic and rectal resection was seen in 2.3% (4/177) and 2.4% (2/84), respectively. Complication and leak rate was similar in the two participating centers. Postoperative fever and elevated CRP levels were significantly correlated to AL. There was no significant risk factor for AL on multivariate analysis. Conclusion: Application of an autologous-derived platelet-rich fibrin matrix (Obsidian ASG®) at anastomotic site following colorectal resection is safe and associated with a low rate of anastomotic leakage.

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To Evaluate the Efficacy and Safety of Autologous Platelet Rich Fibrin Matrix in the Treatment of Non-Healing Ulcer of Various Aetiology

Journal of Clinical and Cosmetic Dermatology ◽

10.16966/2576-2826.147 ◽

2020 ◽

Vol 4 (2) ◽

Author(s):

Shreyansh B ◽

Mehta M ◽

Dilip K

Keyword(s):

Efficacy And Safety ◽

Healing Ulcer ◽

Platelet Rich Fibrin ◽

Fibrin Matrix ◽

Autologous Platelet

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A comparative biomechanical study of repaired tendons wrapped with two biological matrices in Bucks

The Iraqi Journal of Veterinary Medicine ◽

10.30539/iraqijvm.v40i1.141 ◽

2016 ◽

Vol 40 (1) ◽

pp. 73-78

Author(s):

N. H. AL-Falahi

Keyword(s):

Extracellular Matrix ◽

Tensile Strength ◽

Matrix Group ◽

Biological Matrices ◽

Control Groups ◽

Platelet Rich Fibrin ◽

Fibrin Matrix ◽

Superficial Digital Flexor Tendon ◽

And Control ◽

Autologous Platelet

This study is planned to evaluate the efficacy of two biological matrices represented by autologous platelet rich fibrin matrix, as well as a cross linked decellularized caprine pericardial extracellular matrix on enhancing healing of the experimentally severed superficial digital flexor tendon in a goat model. It was carried out on 48 adult apparently healthy bucks, which were divided randomly into three equal groups. Under the effect of sedative and local ring block anesthesia, superficial digital flexor tendon was severed at the mid metacarpal region of the right forelimb. In the first control group, tenorrhaphy was performed and left without additives. While in the second group the tenorrhaphy site was wrapped with a previously prepared autologous platelet rich fibrin strips, as well as in the third group the tenorrhaphy site was wrapped with a cross linked decellularized pericardial extracellular matrix strip which was prepared from the whole fresh caprine pericardium obtained from the slaughter house. Both matrices were fixed in their position at the tenorrhaphy site by few interrupted stitches. The biomechanical evaluation of the operated tendon indicated an increase in tensile strength with time in all groups, but the comparisons among groups showed a significant (P≤0.05) increase at day 15 in both treated as compared to control animals. On day 45 the pericardial extracellular matrix group showed a significant increase in tensile strength as compared to platelet rich fibrin matrix and control groups, but at day 75 there were no differences among groups, at day 180 the pericardial extracellular matrix group showed a significant increase in the tensile strength as compared to platelet rich fibrin matrix and control groups. In conclusion, both biological matrices led to improvement in the biomechanical properties of the operated tendons with time.

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Autologous Platelet-Rich Fibrin Matrix–Augmented Repair for Parameniscal Cysts: Surgical Technique

Arthroscopy Techniques ◽

10.1016/j.eats.2021.07.005 ◽

2021 ◽

Author(s):

Daniele Screpis ◽

Simone Natali ◽

Gianluca Piovan ◽

Venanzio Iacono ◽

Stefano Magnanelli ◽

...

Keyword(s):

Surgical Technique ◽

Platelet Rich Fibrin ◽

Fibrin Matrix ◽

Autologous Platelet

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Histologic changes in early colonic anastomotic healing using autologous platelet-rich fibrin matrix

European Surgery ◽

10.1007/s10353-019-0578-9 ◽

2019 ◽

Vol 52 (4) ◽

pp. 155-164

Author(s):

Bernhard Dauser ◽

Wolf Heitland ◽

Franz G. Bader ◽

Walter Brunner ◽

Yael Nir ◽

...

Keyword(s):

Anastomotic Healing ◽

Platelet Rich Fibrin ◽

Fibrin Matrix ◽

Histologic Changes ◽

Colonic Anastomotic Healing ◽

Autologous Platelet

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The use of autologous platelet-rich fibrin matrix combined with meniscal repair in the treatment of parameniscal cyst: clinical results and cyst recurrence after 2-year of follow up

Journal of Experimental Orthopaedics ◽

10.1186/s40634-021-00423-1 ◽

2021 ◽

Vol 8 (1) ◽

Author(s):

Daniele Screpis ◽

Gianluca Piovan ◽

Simone Natali ◽

Stefano Pasqualotto ◽

Stefano Magnanelli ◽

...

Keyword(s):

Meniscal Repair ◽

Clinical Results ◽

Open Approach ◽

Knee Score ◽

Platelet Rich Fibrin ◽

Fibrin Matrix ◽

Lysholm Knee Score ◽

Autologous Platelet ◽

Parameniscal Cyst

Abstract Purpose Parameniscal cysts are associate with horizontal meniscal tears. Arthroscopic meniscal repair and the excision of the cyst by mini-open approach represent a valid treatment. However, the recurrence of cyst is still a current issue. Therefore, biological factors may be considered to promote the biological repair and avoid recurrence. The aim of the present study was to report the clinical results and the rate of recurrence of the cyst after minimum 2-year of follow up in a cohort of patients treated by meniscal repair and autologous platelet-rich fibrin matrix augment. Methods Patients with lateral parameniscal cyst undergoing arthroscopic meniscal repair and autologous platelet-rich fibrin matrix augment between 2016 and 2019 were retrospectively reviewed in March 2021. Inclusion criteria were absence of prior surgery on the affected knee with minimum 2-year of follow-up. Exclusion criteria were concomitant ligament lesions, rheumatic diseases and knee osteoarthritis. After reviewing the database, each selected patient was contacted and asked to participate in the study; at the follow-up evaluation all patient signed an informed consent. Tegner-Lysholm knee score, IKDC and NRS were collected before surgery and at follow-up. Results This study included 15 patients (8 male) with mean age of 32.8 years old. No recurrence of the cysts was observed. The Tegner-Lysholm knee score and IKDC subjective scores increased respectively from 41.3 ± 5.4 and 37.6 ± 5.1 at baseline to 92.3 ± 4.6 and 89.4 ± 2.6 at the final follow up. Concerning pain relief, the Numeric Pain Rating Scale (NRS) displayed a significant improvement reaching at the follow up a score of 1,3 ± 1.1 in comparison to 6.8 ± 0.9 at the baseline. Conclusion Surgical management of symptomatic lateral parameniscal cyst with cyst excision, autologous PRP membrane application and meniscus repair demonstrated excellent subjective clinical outcome with any cyst reoccurrence. Level of evidence III, retrospective cohort study.

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