Evaluation of cell viability of human dental pulp stem cells in two dimensional and three dimensional fibrin glue scaffold

This study aimed to investigate the cytotoxicity and bioactivity of a novel nanocomposite containing nanoparticles of bioactive glass (nBGs) on human dental pulp stem cells (hDPSCs). nBGs were synthesized by the sol–gel method. Biodentine (BD) nanocomposites (nBG/BD) were prepared with 2 and 5% wt of nBG content; unmodified BD and glass ionomer cement were used as references. Cell viability and attachment were evaluated after 3, 7 and 14 days. Odontogenic differentiation was assessed with alkaline phosphatase (ALP) activity after 7 and 14 days of exposure. Cells successfully adhered and proliferated on nBG/BD nanocomposites, cell viability of nanocomposites was comparable with unmodified BD and higher than GIC. nBG/BD nanocomposites were, particularly, more active to promote odontogenic differentiation, expressed as higher ALP activity of hDPSCs after 7 days of exposure, than neat BD or GIC. This novel nanocomposite biomaterial, nBG/BD, allowed hDPSC attachment and proliferation and increased the expression of ALP, upregulated in mineral-producing cells. These findings open opportunities to use nBG/BD in vital pulp therapies.

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Osteogenic differentiation of human dental pulp stem cells on β-tricalcium phosphate/poly (l-lactic acid/caprolactone) three-dimensional scaffolds

Journal of Tissue Engineering ◽

10.1177/2041731412467998 ◽

2012 ◽

Vol 3 (1) ◽

pp. 204173141246799 ◽

Cited By ~ 33

Author(s):

Rashi Khanna-Jain ◽

Bettina Mannerström ◽

Annukka Vuorinen ◽

George KB Sándor ◽

Riitta Suuronen ◽

...

Keyword(s):

Stem Cells ◽

Lactic Acid ◽

Osteogenic Differentiation ◽

Tricalcium Phosphate ◽

Dental Pulp ◽

Three Dimensional ◽

Dental Pulp Stem Cells ◽

Human Dental Pulp

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Bioprinting of three-dimensional dentin–pulp complex with local differentiation of human dental pulp stem cells

Journal of Tissue Engineering ◽

10.1177/2041731419845849 ◽

2019 ◽

Vol 10 ◽

pp. 204173141984584 ◽

Cited By ~ 14

Author(s):

Jonghyeuk Han ◽

Da Sol Kim ◽

Ho Jang ◽

Hyung-Ryong Kim ◽

Hyun-Wook Kang

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Dental Pulp ◽

Three Dimensional ◽

Dental Pulp Stem Cells ◽

Patient Specific ◽

Fibrinogen Concentration ◽

Dental Tissues ◽

Human Dental Pulp ◽

Dental Pulp Stem Cell

Numerous approaches have been introduced to regenerate artificial dental tissues. However, conventional approaches are limited when producing a construct with three-dimensional patient-specific shapes and compositions of heterogeneous dental tissue. In this research, bioprinting technology was applied to produce a three-dimensional dentin–pulp complex with patient-specific shapes by inducing localized differentiation of human dental pulp stem cells within a single structure. A fibrin-based bio-ink was designed for bioprinting with the human dental pulp stem cells. The effects of fibrinogen concentration within the bio-ink were investigated in terms of printability, human dental pulp stem cell compatibility, and differentiation. The results show that micro-patterns with human dental pulp stem cells could be achieved with more than 88% viability. Its odontogenic differentiation was also regulated according to the fibrinogen concentration. Based on these results, a dentin–pulp complex having patient-specific shape was produced by co-printing the human dental pulp stem cell–laden bio-inks with polycaprolactone, which is a bio-thermoplastic used for producing the overall shape. After culturing with differentiation medium for 15 days, localized differentiation of human dental pulp stem cells in the outer region of the three-dimensional cellular construct was successfully achieved with localized mineralization. This result demonstrates the possibility to produce patient-specific composite tissues for tooth tissue engineering using three-dimensional bioprinting technology.

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Nano-hydroxy apatite/chitosan/gelatin scaffolds enriched by a combination of platelet-rich plasma and fibrin glue enhance proliferation and differentiation of seeded human dental pulp stem cells

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2018.11.072 ◽

2019 ◽

Vol 109 ◽

pp. 1924-1931 ◽

Cited By ~ 5

Author(s):

Ali Sadeghinia ◽

Soodabeh Davaran ◽

Roya Salehi ◽

Zahra Jamalpoor

Keyword(s):

Stem Cells ◽

Fibrin Glue ◽

Dental Pulp ◽

Platelet Rich Plasma ◽

Dental Pulp Stem Cells ◽

Hydroxy Apatite ◽

Proliferation And Differentiation ◽

Human Dental Pulp

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Effects of two fast-setting pulp-capping materials on cell viability and osteogenic differentiation in human dental pulp stem cells: An in vitro study

Archives of Oral Biology ◽

10.1016/j.archoralbio.2019.02.014 ◽

2019 ◽

Vol 100 ◽

pp. 100-105 ◽

Cited By ~ 1

Author(s):

Yan Sun ◽

Jun Liu ◽

Tao Luo ◽

Ya Shen ◽

Ling Zou

Keyword(s):

Stem Cells ◽

Cell Viability ◽

Osteogenic Differentiation ◽

Dental Pulp ◽

In Vitro Study ◽

Dental Pulp Stem Cells ◽

Vitro Study ◽

Pulp Capping ◽

Human Dental Pulp

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Simvastatin increases cell viability and suppresses the expression of cytokines and vascular endothelial growth factor in inflamed human dental pulp stem cells in vitro

Advances in Clinical and Experimental Medicine ◽

10.17219/acem/75776 ◽

2018 ◽

Vol 27 (12) ◽

pp. 1615-1623 ◽

Cited By ~ 3

Author(s):

Dai Xue ◽

Zhongjian Gong ◽

Fangyong Zhu ◽

Yanjing Qiu ◽

Xiaodan Li

Keyword(s):

Stem Cells ◽

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Cell Viability ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Vascular Endothelial ◽

Human Dental Pulp ◽

Endothelial Growth Factor

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Effects of Different Calcium Silicate Cements on the Inflammatory Response and Odontogenic Differentiation of Lipopolysaccharide-Stimulated Human Dental Pulp Stem Cells

Materials ◽

10.3390/ma12081259 ◽

2019 ◽

Vol 12 (8) ◽

pp. 1259 ◽

Cited By ~ 2

Author(s):

Minsun Chung ◽

Sukjoon Lee ◽

Dongzi Chen ◽

Ukseong Kim ◽

Yaelim Kim ◽

...

Keyword(s):

Stem Cells ◽

Inflammatory Response ◽

Cell Viability ◽

Calcium Silicate ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Cell Counting ◽

Odontogenic Differentiation ◽

Human Dental Pulp ◽

Calcium Silicate Cements

This study aimed to analyze the effects of different calcium silicate cements (CSCs) on the inflammatory response and odontogenic differentiation of lipopolysaccharide-stimulated human dental pulp stem cells. Human dental pulp stem cells (hDPSCs) were stimulated with lipopolysaccharide (LPS) to induce inflammation. These LPS-induced dental pulp stem cells (LDPSCs) were cultured with ProRoot MTA, Biodentine, Retro MTA, and Dycal. Cell viability was evaluated using the Cell Counting Kit-8 assay. Interleukin (IL)-6, IL-8, and transforming growth factor (TGF)-β1 cytokine levels were assessed using the enzyme-linked immunosorbent assay. The expressions of alkaline phosphatase (ALP), osteocalcin, and runt-related transcription factor 2 (RUNX2) were analyzed through real-time polymerase chain reaction. ProRoot MTA, Biodentine, and Retro MTA did not significantly decrease the cell viability of LDPSCs for up to 48 h (p < 0.05). Retro MTA significantly decreased the expression of IL-6 and IL-8 by LDPSCs. ProRoot MTA and Biodentine significantly reduced TGF-β expression by LDPSCs (p < 0.05). Regarding odontogenic differentiation, all CSCs had no effect on ALP expression but increased the production of RUNX2 at 12 h.

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