Compare and evaluate the antibacterial efficacy of sodium hypochlorite and Calendula officinalis against Streptococcus mutans as a root canal irrigating solution: An in vivo study

2020 ◽  
Vol 12 (1) ◽  
pp. 74
Author(s):  
VirajS Yalgi ◽  
KishorG Bhat
Author(s):  
FaizalC Peedikayi ◽  
Vimal Remy ◽  
Seena John ◽  
TP Chandru ◽  
Prathima Sreenivasan ◽  
...  

Materials ◽  
2020 ◽  
Vol 13 (2) ◽  
pp. 260
Author(s):  
Sung-Chih Hsieh ◽  
Nai-Chia Teng ◽  
Chia Chun Chu ◽  
You-Tai Chu ◽  
Chung-He Chen ◽  
...  

The objective of this study was to evaluate the antibacterial efficacy against Enterococcus faecalis and Streptococcus mutans and in vivo toxicity using embryonic zebrafish assays of sodium hypochlorite (NaOCl) and electrolyzed oxidizing (EO) water (containing hypochlorous acid (HOCl))-based root canal irrigating solutions. Methodology: Using 100 μL microbial count of 1 × 108 cfu/mL Enterococcus faecalis to mix with each 10 mL specimen of NaOCl or HOCl for designed time periods. The above protocol was also repeated for Streptococcus mutans. The concentration of viable microorganisms was estimated based on each standardized inoculum using a plate-count method. Zebrafish embryo assays were used to evaluate acute toxicity. Results: All the HOCl or NaOCl treatment groups showed > 99.9% antibacterial efficacy against Enterococcus faecalis and Streptococcus mutans. Zebrafish embryos showed almost complete dissolution in 1.5% NaOCl within 5 min. Both survival rates after being treated with 0.0125% and 0.0250% HOCl for 0.5 min or 1.0 min were similar to that of E3 medium. Conclusions: Both NaOCl and HOCl revealed similar antibacterial efficacy (> 99.9%) against Enterococcus faecalis and Streptococcus mutans. While 1.5% NaOCl fully dissolved the Zebrafish embryos, both 0.0125% and 0.0250% HOCl showed little in vivo toxicity, affirming its potential as an alternative irrigation solution for vital pulp therapy.


1998 ◽  
Vol 16 (5) ◽  
pp. 263-267 ◽  
Author(s):  
GAVRIEL KESLER ◽  
RUMELIA KOREN ◽  
ANAT KESLER ◽  
NISSIM HAY ◽  
RIVKA GAL

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