Common bunt resistance gene Bt10 located on wheat chromosome 6D

2006 ◽  
Vol 86 (Special Issue) ◽  
pp. 1409-1412 ◽  
Author(s):  
J. G. Menzies ◽  
R. E. Knox ◽  
Z. Popovic ◽  
J. D. Procunier
Keyword(s):  
Microsatellite Markers ◽  
Resistance Gene ◽  
Doubled Haploid ◽  
Chromosomal Location ◽  
Wheat Chromosome ◽  
Common Bunt ◽  
Tilletia Tritici ◽  
Doubled Haploid Lines ◽  
The Individual ◽  
Bunt Resistance

Knowledge of the chromosomal location of disease resistance genes assists in their identification and classification. The determination of the chromosomal location in wheat of the common bunt (Tilletia tritici and T. laevis) resistance gene Bt10 was the goal of this study. Doubled haploid lines were developed from a cross between bunt susceptible Glenlea and bunt resistant AC Taber carrying Bt10. The doubled haploid lines were inoculated with T. tritici race T19, grown in a growth room and rated for bunt near maturity. A series of 50 wheat microsatellite markers were tested on DNA of the individual lines. The population segregated 1:1 for bunt reaction with clear separation between resistant and susceptible classes. A trait related DNA polymorphism generated by gwm469 located in chromosome 6D fit a 1:1 segregation. Combined segregation of bunt resistance and the gwm469 polymorphism differed significantly from a 1:1: 1:1 ratio with a preponderance of parental types confirming linkage of gwm469 with Bt10. The map distance between gwm469 and Bt10was estimated at 19.3 cM by MAPMAKER. The microsatellite markers wmc749, barc54 and cfd0033, located on chromosome 6D, also were significantly associated with the bunt resistance and gwm469. In total, six markers previously located to chromosome 6D were in the linkage group with the Bt10 common bunt resistance. The linkage of these markers with each other and Bt10 indicated that Bt10 is located on the short arm of chromosome 6D. Key words: Tilletia tritici, Tilletia laevis, Triticum aestivum, wheat, microsatellite, doubled haploid

A DNA marker for the Bt-10 common bunt resistance gene in wheat

Genome ◽  
1996 ◽  
Vol 39 (1) ◽  
pp. 51-55 ◽  
Author(s):  
T. Demeke ◽  
A. Laroche ◽  
D. A. Gaudet
Keyword(s):  
Resistance Gene ◽  
Isogenic Line ◽  
Near Isogenic Line ◽  
Common Bunt ◽  
Resistant Parent ◽  
Rapd Primer ◽  
Susceptible Allele ◽  
The Common ◽  
Dna Fragment ◽  
Bunt Resistance

The Bt-10 bunt gene confers resistance to most races of the common bunt fungi, Tilletia tritici and T. laevis. The RAPD technique, employing a total of 965 decamer primers, was used to identify polymorphic markers between resistant (BW553) and susceptible ('Neepawa') near-isogenic lines. Primer 196 (5′ CTC CTC CCC C 3′) produced a 590 base pair (bp) reproducible fragment only in the resistant near-isogenic line. The 590-bp DNA fragment was present in all the 22 wheat cultivars known to carry the Bt-10 resistance gene and also in 15 resistant F2 lines obtained from a cross between the resistant parent, BW553, and the susceptible parent, 'Neepawa'. The 590-bp fragment was absent in 16 susceptible cultivars tested and in 15 susceptible F2 lines obtained from the cross described above. These results suggest a close linkage between the presence of the 590-bp fragment and the Bt-10 resistance gene. Primer 372 (5′ CCC ACT GAC G 3′) amplified a 1.0-kilobase (kb) fragment that was present only in the susceptible near-isogenic line. This 1.0-kb fragment was present in 13 of the 16 susceptible cultivars and in 13 of the 15 susceptible F2 lines. However, the primer also amplified the 1.0-kb fragment in some resistant cultivars and resistant F2 lines, suggesting a looser linkage between the occurrence of the fragment and the susceptible allele. Key words : RAPD, primer, Bt-10 bunt resistance gene, wheat, marker.

Inheritance of Common Bunt Resistance in Androgenetically Derived Doubled Haploid and Random Inbred Populations of Wheat

Crop Science ◽  
1998 ◽  
Vol 38 (5) ◽  
pp. 1119-1124 ◽  
Author(s):  
R. E. Knox ◽  
M. R. Fernandez ◽  
A. L. Brûlé ◽  
R. M. DePauw
Keyword(s):  
Doubled Haploid ◽  
Common Bunt ◽  
Inbred Populations ◽  
Bunt Resistance

scholarly journals Mapping of common bunt resistance gene Bt9 in wheat

2017 ◽  
Vol 130 (5) ◽  
pp. 1031-1040 ◽  
Author(s):  
Philipp Matthias Steffan ◽  
Anna Maria Torp ◽  
Anders Borgen ◽  
Gunter Backes ◽  
Søren K. Rasmussen
Keyword(s):  
Resistance Gene ◽  
Common Bunt ◽  
Bunt Resistance

Construction of three linkage maps in bread wheat, Triticum aestivum

2001 ◽  
Vol 52 (12) ◽  
pp. 1089 ◽  
Author(s):  
K. J. Chalmers ◽  
A. W. Campbell ◽  
J. Kretschmer ◽  
A. Karakousis ◽  
P. H. Henschke ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Doubled Haploid ◽  
Wheat Breeding ◽  
Genetic Maps ◽  
Aflp Markers ◽  
Linkage Maps ◽  
Linkage Groups ◽  
D Genome ◽  
Doubled Haploid Lines ◽  
Wide Range

Genetic maps were compiled from the analysis of 160–180 doubled haploid lines derived from 3 crosses: Cranbrook Halberd, CD87 Katepwa, and Sunco Tasman. The parental wheat lines covered a wide range of the germplasm used in Australian wheat breeding. The linkage maps were constructed with RFLP, AFLP, microsatellite markers, known genes, and proteins. The numbers of markers placed on each map were 902 for Cranbrook Halberd, 505 for CD87 Katepwa, and 355 for Sunco Tasman. Most of the expected linkage groups could be determined, but 10–20% of markers could not be assigned to a specific linkage group. Homologous chromosomes could be aligned between the populations described here and linkage groups reported in the literature, based around the RFLP, protein, and microsatellite markers. For most chromosomes, colinearity of markers was found for the maps reported here and those recorded on published physical maps of wheat. AFLP markers proved to be effective in filling gaps in the maps. In addition, it was found that many AFLP markers defined specific genetic loci in wheat across all 3 populations. The quality of the maps and the density of markers differs for each population. Some chromosomes, particularly D genome chromosomes, are poorly covered. There was also evidence of segregation distortion in some regions, and the distribution of recombination events was uneven, with substantial numbers of doubled haploid lines in each population displaying one or more parental chromosomes. These features will affect the reliability of the maps in localising loci controlling some traits, particularly complex quantitative traits and traits of low heritability. The parents used to develop the mapping populations were selected based on their quality characteristics and the maps provide a basis for the analysis of the genetic control of components of processing quality. However, the parents also differ in resistance to several important diseases, in a range of physiological traits, and in tolerance to some abiotic stresses.

Resistance to an imidazolinone herbicide is conferred by a gene on chromosome 6DL in the wheat line cv. 9804

Weed Science ◽  
2004 ◽  
Vol 52 (1) ◽  
pp. 83-90 ◽  
Author(s):  
James A. Anderson ◽  
Leanne Matthiesen ◽  
Justin Hegstad
Keyword(s):  
Microsatellite Markers ◽  
Resistance Gene ◽  
Common Wheat ◽  
Herbicide Resistance ◽  
Chromosomal Location ◽  
Natural Hybridization ◽  
Recurrent Parent ◽  
D Genome ◽  
Jointed Goatgrass ◽  
The Common

An induced mutation of the common wheat (2n = 6x = 42, AABBDD genomes) cultivar ‘Fidel’ has been shown to provide resistance to the imidazolinone class of herbicides. This class of herbicide gives broad-spectrum weed control including the weedy relative of wheat, jointed goatgrass (2n = 4x = 28, CCDD genomes). Because wheat and jointed goatgrass share a common genome, genes present on the D genome may transfer between the two species as a result of natural hybridization and selective pressures. Our objectives were to determine which genome of common wheat contained the herbicide resistance gene in the mutated Fidel and to genetically map its position. We investigated the chromosomal location of this gene using both durum (2n = 4x = 28, AABB genomes) and common wheat (6x) backgrounds. From crosses of durum wheat genotypes as the recurrent parent with mutated Fidel (cv. 9804, resistant), only BC1plants containing chromosome 6D (inherited from cv. 9804) were resistant to applications of labeled rates of imazamox, an imidazolinone herbicide. No other D-genome chromosome was absolutely associated with herbicide resistance. To confirm this chromosomal location and genetically map the position of this gene, two populations of F3families from the cross of cv. 9804 to the common wheat cultivars ‘Cashup’ and ‘Madsen’ were screened for reaction to imazamox, followed by genetic mapping with microsatellite markers. Two linked microsatellite markers were associated with the resistance trait, and one of them,Xgdm127, was located to chromosome 6D using aneuploid stocks, confirming the location of this gene on 6D. These results indicate that this resistance gene is in the genome that common wheat shares with jointed goatgrass. Therefore, imidazolinone-resistant wheat will need to be carefully managed to minimize the occurrence and spread of resistant jointed goatgrass, whether such plants arise because of hybridization with resistant common wheat or by independent mutation, a frequent occurrence with this herbicide class.

Markers to a common bunt resistance gene derived from ‘Blizzard’ wheat (Triticum aestivum L.) and mapped to chromosome arm 1BS

2009 ◽  
Vol 119 (3) ◽  
pp. 541-553 ◽  
Author(s):  
Shu Wang ◽  
Ronald E. Knox ◽  
Ronald M. DePauw ◽  
Fran R. Clarke ◽  
John M. Clarke ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Resistance Gene ◽  
Triticum Aestivum L ◽  
Common Bunt ◽  
Chromosome Arm ◽  
Bunt Resistance

scholarly journals Analysis of the intralinear polymorphism and the homozygosity rate of triticale doubled haploid lines by microsatellite markers

2020 ◽  
Vol 64 (2) ◽  
pp. 199-208
Author(s):  
A. V. Lagunovskaya ◽  
A. A. Buracova ◽  
V. N. Bushtevich ◽  
V. I. Sakovich ◽  
V. A. Lemesh ◽  
...  
Keyword(s):  
Cluster Analysis ◽  
Microsatellite Markers ◽  
Somaclonal Variation ◽  
Ssr Markers ◽  
Doubled Haploid ◽  
Microsatellite Loci ◽  
Doubled Haploids ◽  
Hexaploid Triticale ◽  
Doubled Haploid Lines

We evaluated the rate of polymorphism of doubled haploid lines of hexaploid triticale obtained by the method of anther culture based on hybrids of spring and winter types. Using 7 SSR markers for the loci on the chromosomes A- (Xgwm186, Xgwm291, Xgwm595) and B- (Xgwm371, Xgwm540, Xgwm554, Xgwm234), polymorphism of 38 doubled haploid lines of hexaploid triticale was studied. Interlinear polymorphism along six microsatellite loci except the Xgwm554 locus, which is not polymorphic in the studied doubled haploid lines, was revealed. The highest polymorphism was observed for the Xgwm186, Xgwm291 and Xgwm595 loci. The cluster analysis showed that all studied lines were divided into three main groups. The origin of the lines did not affect the distribution in groups. This confirms the influence of in vitro culture somaclonal variation. Eight lines of doubled haploids, which are heterozygous for one of the studied microsatellite loci, were identified. We showed the possibility of using SSR markers to assess interlinear polymorphism and the homozygosity rate in the triticale doubled haploid lines obtained by the method of induced androgenesis in vitro.

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