Sequencing and phylogenetic characterization of a novel RNA virus in Arma chinensis

2021 ◽  
Vol 65 (03) ◽  
pp. 320-323
Author(s):  
Yonghao Dong ◽  
Pengjun Xu ◽  
Guangwei Ren ◽  
Changchun Feng ◽  
Dongyang Liu ◽  
...  
2020 ◽  
Vol 47 (5) ◽  
pp. 4015-4019
Author(s):  
Yingjie Liu ◽  
Guangwei Ren ◽  
Lianqiang Jiang ◽  
Changchun Feng ◽  
Dongyang Liu ◽  
...  

2012 ◽  
Vol 43 (3) ◽  
pp. 640-644 ◽  
Author(s):  
Anuwat Wiratsudakul ◽  
Ladawan Sariya ◽  
Phirom Prompiram ◽  
Siriporn Tantawet ◽  
Duangkhamol Suraruangchai ◽  
...  

2016 ◽  
Vol 219 ◽  
pp. 92-99 ◽  
Author(s):  
Kunfei Li ◽  
Dan Zheng ◽  
Jiasen Cheng ◽  
Tao Chen ◽  
Yanping Fu ◽  
...  

2021 ◽  
Author(s):  
Yang Sun ◽  
Yan qiong Li ◽  
Wen han Dong ◽  
Ai li Sun ◽  
Ning wei Chen ◽  
...  

Abstract The complete genome of the dsRNA virus isolated from Rhizoctonia solani AG-1 IA 9–11 (designated as Rhizoctonia solani dsRNA virus 11, RsRV11 ) were determined. The RsRV11 genome was 9,555 bp in length, contained three conserved domains, SMC, PRK and RT-like super family, and encoded two non-overlapping open reading frames (ORFs). ORF1 potentially coded for a 204.12 kDa predicted protein, which shared low but significant amino acid sequence identities with the putative protein encoded by Rhizoctonia solani RNA virus HN008 (RsRV-HN008) ORF1. ORF2 potentially coded for a 132.41 kDa protein which contained the conserved motifs of the RNA-dependent RNA polymerase (RdRp). Phylogenetic analysis indicated that RsRV11 was clustered with RsRV-HN008 in a separate clade independent of other virus families. It implies that RsRV11, along with RsRV-HN008 possibly a new fungal virus taxa closed to the family Megabirnaviridae, and RsRV11 is a new member of mycoviruses.


PLoS ONE ◽  
2018 ◽  
Vol 13 (10) ◽  
pp. e0206382 ◽  
Author(s):  
Sun-Jung Kwon ◽  
Gug-Seoun Choi ◽  
Boram Choi ◽  
Jang-Kyun Seo

2013 ◽  
Vol 30 (3) ◽  
pp. 521-529 ◽  
Author(s):  
L. L. Oliveira ◽  
R. B. Costa ◽  
I. K. Sakamoto ◽  
I. C. S. Duarte ◽  
E. L. Silva ◽  
...  

Author(s):  
Mehrdad Halaji ◽  
Shahrzad Shahidi ◽  
Behrooz Ataei ◽  
Abdolamir Atapour ◽  
Awat Feizi ◽  
...  

Abstract Background This study aimed to investigate the phylogenetic characterization and virulence traits of uropathogenic Escherichia coli (UPEC) isolated from kidney transplant patients (KTPs) as well as non-KTPs and analyze the clonal distribution of Extended spectrum β-lactamases (ESBLs)-producing UPEC containing blaCTX-M gene. Methods To this end, we determined virulence marker and the phylogenetic characterization of UPEC in non-KTPs (n = 65) and KTPs (n = 46). The non-KTPs were considered the control group of the study. Also, according to the Achtman scheme, we performed multilocus sequence typing to assess the relationship between twenty-nine of ESBL-producing isolates containing blaCTX-M gene. Results According to the results of PCR assay, the prevalence of virulence factor genes ranged from 0% (cnf and papG III) to 93.7% (fimH). Also, KTP isolates significantly differed from non-KTP isolates only in terms of the prevalence of pap GI elements. Moreover, the most frequent UPEC isolates were in phylogenetic group B2, followed by group D (18.9%), and group A (13.5%). Furthermore, except for phylogenetic group C, there was no significant correlation between phylogenetic distribution in KTPs and non-KTPs. Additionally, MLST analysis of blaCTX-M carrying isolates identified 18 unique sequence types (ST) the most common of which was ST131 (24.1%), followed by ST1193 (10.3%), while fourteen STs were detected only once. Conclusions The results further revealed significant differences between the UPEC isolates from KTPs and non-KTPs regarding the phylogroups C and PAI gene. Based on MLST analysis, we also observed a relatively high diversity in UPEC isolates obtained from KTPs and non-KTPs. Moreover, clonal complex (CC) 131 and ST131 were found to be the most prevalent clones and ST types, respectively. Besides, for the first time, ST8503 were reported in KTPs. These results suggested regular studies on characterization of UPEC isolates among KTPs.


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