Prevalence of high-risk human papillomavirus types (HPV-16, HPV-18) and their physical status in primary laryngeal squamous cell carcinoma

Background: This study investigated prospectively the role of human papillomavirus (HPV) in paranasal inverted papilloma (IP). Methods: HPV presence and viral load and physical status of HPV-16 were examined by polymerase chain reaction-based methods using fresh frozen samples obtained from 13 patients with IP (IP group), 11 with squamous cell carcinoma in the maxillary sinus (SCC group) and 39 with chronic inflammatory lesions (inflammatory group). Results: The presence of the HPV genome was detected in 46.1%, 27.3% and 7.6% of patients in the IP, SCC and inflammatory groups, respectively. The IP group showed significantly higher HPV-positive rates than the inflammatory group. All types of HPV detected were high-risk HPV, especially HPV-16. The relative HPV-16 copy numbers varied from 2.5 to 1524.1 per 50 ng genomic DNA. The viral load was higher in the IP and SCC groups than in the inflammatory group. In the IP group, no significant relationship was found between HPV-16 viral load and clinical characteristics, or between physical status and clinical characteristics. One patient with IP and concomitant squamous cell carcinoma, however, showed high viral load and integration. Conclusions: HPV infection is involved in the pathogenesis of IP, and high viral load and integration of HPV have an important role in malignant lesion in association with IP.

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Frequency and genotype distribution of high risk human papillomavirus in esophageal squamous cell carcinoma

The Journal of Infection in Developing Countries ◽

10.3855/jidc.12487 ◽

2020 ◽

Vol 14 (11) ◽

pp. 1320-1326

Author(s):

Nuzhat Sultana ◽

Walayat Shah ◽

Waheed Iqbal ◽

Shahid Pervez ◽

Ikramullah Khan ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Human Papillomavirus ◽

High Risk ◽

Esophageal Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Tertiary Care ◽

Genotype Distribution ◽

Specific Primers ◽

Hpv 16

Introduction: Esophageal squamous cell carcinoma (ESCC) is a dismal disease exhibiting striking geographical differences in its incidence. It is multifactorial in origin. Among infectious agents, human papillomavirus (HPV) was introduced as a possible causative agent in the development of ESCC in 1982. Subsequent studies using various methods have confirmed the presence of HPV in ESCC. We aimed to determine the frequency of HPV in ESCC in northwest Pakistan which is part of high risk belt for this disease. Methodology: This study was conducted on two hundred and forty-three (243) diagnosed cases of ESSC at two tertiary care hospitals of Peshawar, Pakistan, from 2011 to 2016. DNA was extracted from all specimens. Polymerase chain reaction (PCR) was used to check the quality of DNA using β-globin primers and frequency and genotypes of HPV using HPV general primers and type-specific primers respectively. HPV and its genotypes were confirmed through the sequencing of a few selected cases. Results: Two hundred and three (203) tissue specimens had adequate DNA and were further analyzed. HPV positivity with general primers alone was 15.7% (32/203). Using HPV general primers and type-specific primers (HPV 16 or HPV 18), the overall positivity of HPV was 31% (63/203). For type-specific primers, frequency of HPV types 16 and 18 was 20.19% (41) and 7.8% (16) respectively where 6 cases were positive for both HPV 16 and 18. Conclusions: The overall high prevalence of HPV indicates it as a possible risk factor for ESSC.

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The role of high-risk human papillomavirus infections in laryngeal squamous cell carcinoma

European Archives of Oto-Rhino-Laryngology ◽

10.1007/s00405-017-4718-1 ◽

2017 ◽

Vol 274 (11) ◽

pp. 3837-3842 ◽

Cited By ~ 8

Author(s):

Claus Wittekindt ◽

Nora Wuerdemann ◽

Stefan Gattenlöhner ◽

Alexander Brobeil ◽

Malgorzata Wierzbicka ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Human Papillomavirus ◽

High Risk ◽

Cell Carcinoma ◽

Squamous Cell ◽

Laryngeal Squamous Cell Carcinoma ◽

Papillomavirus Infections

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Human papillomavirus load and physical status in sinonasal inverted papilloma and squamous cell carcinoma

Rhinology Journal ◽

10.4193/rhino11.106 ◽

2012 ◽

Vol 50 (1) ◽

pp. 87-94

Author(s):

Masahiro Hasegawa ◽

Zeyi Deng ◽

Hiroyuki Maeda ◽

Yukashi Yamashita ◽

Sen Matayoshi ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Human Papillomavirus ◽

Viral Load ◽

Cell Carcinoma ◽

Squamous Cell ◽

Clinical Characteristics ◽

High Viral Load ◽

Inverted Papilloma ◽

Physical Status ◽

Hpv 16

Background: This study investigated prospectively the role of human papillomavirus (HPV) in paranasal inverted papilloma (IP). Methods: HPV presence and viral load and physical status of HPV-16 were examined by polymerase chain reaction-based methods using fresh frozen samples obtained from 13 patients with IP (IP group), 11 with squamous cell carcinoma in the maxillary sinus (SCC group) and 39 with chronic inflammatory lesions (inflammatory group). Results: The presence of the HPV genome was detected in 46.1%, 27.3% and 7.6% of patients in the IP, SCC and inflammatory groups, respectively. The IP group showed significantly higher HPV-positive rates than the inflammatory group. All types of HPV detected were high-risk HPV, especially HPV-16. The relative HPV-16 copy numbers varied from 2.5 to 1524.1 per 50 ng genomic DNA. The viral load was higher in the IP and SCC groups than in the inflammatory group. In the IP group, no significant relationship was found between HPV-16 viral load and clinical characteristics, or between physical status and clinical characteristics. One patient with IP and concomitant squamous cell carcinoma, however, showed high viral load and integration. Conclusions: HPV infection is involved in the pathogenesis of IP, and high viral load and integration of HPV have an important role in malignant lesion in association with IP.

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Epidermodysplasia verruciformis with keratoacanthoma, Bowen's disease and squamous cell carcinoma: isolation of high-risk types of HPV 5 and unknown type of human papillomavirus

Journal of the European Academy of Dermatology and Venereology ◽

10.1111/j.1468-3083.2007.02526.x ◽

2008 ◽

Vol 22 (9) ◽

pp. 1126-1127 ◽

Cited By ~ 11

Author(s):

T Mitsuishi ◽

K Ohara ◽

T Suzuki ◽

T Mochizuki ◽

T Kaneko ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Human Papillomavirus ◽

High Risk ◽

Cell Carcinoma ◽

Squamous Cell ◽

Epidermodysplasia Verruciformis ◽

Bowen’S Disease ◽

Bowen's Disease ◽

Unknown Type

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Profiling high‐risk human papillomavirus (HPV) subtypes in oral squamous cell carcinoma (OSCC)

Histopathology ◽

10.1111/his.14504 ◽

2021 ◽

Vol 79 (S1) ◽

pp. 23-24

Keyword(s):

Squamous Cell Carcinoma ◽

Human Papillomavirus ◽

High Risk ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell

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Digital squamous cell carcinoma and association with diverse high-risk human papillomavirus types

Journal of the American Academy of Dermatology ◽

10.1016/j.jaad.2009.10.044 ◽

2011 ◽

Vol 64 (5) ◽

pp. 981-985 ◽

Cited By ~ 26

Author(s):

Rachel H. Gormley ◽

Caroline M. Groft ◽

Christopher J. Miller ◽

Carrie L. Kovarik

Keyword(s):

Squamous Cell Carcinoma ◽

Human Papillomavirus ◽

High Risk ◽

Cell Carcinoma ◽

Squamous Cell

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Association of Cystic Neck Metastases and Human Papillomavirus–Positive Oropharyngeal Squamous Cell Carcinoma

Archives of Pathology & Laboratory Medicine ◽

10.5858/133.11.1798 ◽

2009 ◽

Vol 133 (11) ◽

pp. 1798-1803

Author(s):

Jonathan B. McHugh

Keyword(s):

Squamous Cell Carcinoma ◽

Human Papillomavirus ◽

High Risk ◽

Cell Carcinoma ◽

Head And Neck ◽

Squamous Cell ◽

Sexual Behaviors ◽

Oropharyngeal Squamous Cell Carcinoma ◽

Treatment Modalities ◽

Neck Metastases

Abstract Human papillomavirus is an established cause of oropharyngeal squamous cell carcinoma. Similar to cervical cancer, these cancers are usually caused by high-risk human papillomavirus types 16 and 18 and are associated with high-risk sexual behaviors. Human papillomavirus–associated oropharyngeal squamous cell carcinoma typically affects the palatine and lingual tonsils and frequently results in cystic neck metastases. The histopathology of this subset of head and neck squamous cell carcinoma is unique and typically characterized by poorly differentiated, nonkeratinizing morphology with a basaloid appearance. These tumors occur in younger patients and are more often seen in nonsmokers compared with conventional oral cavity and oropharyngeal squamous cell carcinomas. The incidence of human papillomavirus–associated squamous cell carcinoma is increasing. Recognition of this unique clinicopathologic subset of head and neck carcinoma is important because these patients typically respond more favorably to organ-sparing treatment modalities and have an improved prognosis.

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